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(Submitter supplied) The restoration of catalytic activity to mutant enzymes by small molecules is well-established for in vitro systems. Here we show that the protein tyrosine kinase Src R388A mutant can be rescued in live cells using the small molecule imidazole. Cellular rescue of a v-Src homolog was rapid and reversible and conferred predicted oncogenic properties. Using chemical rescue in combination with mass spectrometry, six known Src kinase substrates were confirmed, and several new protein targets identified. more...

Organism:

Mus musculus

Type:

Expression profiling by array

Dataset:

GDS1788

Platform:

GPL1261

6 Samples

Download data: CEL, XLS

Analysis of protein tyrosine kinase Src R388A mutant protein in living cells at 1 and 12 hours after chemical rescue with imidazole. Rapid and reversible activity stimulation is an advantage chemical rescue has over inducible transcriptional systems and temperature-sensitive mutants.

Organism:

Mus musculus

Type:

Expression profiling by array, count, 2 cell line, 3 time sets

Platform:

GPL1261

Series:

GSE4043

6 Samples

Download data: CEL

(Submitter supplied) Affymetrix submissions are typically submitted to GEO using the GEOarchive method described at http://www.ncbi.nlm.nih.gov/projects/geo/info/geo_affy.html June 03, 2009: annotation table updated with netaffx build 28 June 07, 2012: annotation table updated with netaffx build 32 June 23, 2016: annotation table updated with netaffx build 35 Protocol: see manufacturer's web site All probe sets represented on the GeneChip Mouse Expression Set 430 are included on the GeneChip Mouse Genome 430 2.0 Array. more...

Organism:

Mus musculus

601 DataSets

4547 Series

40 Related Platforms

58950 Samples

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