GEO DataSets

(Submitter supplied) DNA microarray analysis of genes regulated by the alternative sigma factor, sigma 32 (rpoH). Sigma 32-dependent genes were initially identified by comparing a wild type (wt) E. coli K-12 strain that has a low level of sigma 32, with a strain over-expressing sigma 32 (following induction of rpoH from an inducible promoter by IPTG). We monitored changes in gene expression in 4 separate time-courses. Because sigma 32 is negatively regulated, its activity decreases 10 min after overexpression. more...

Organism:

Escherichia coli

Type:

Expression profiling by array

Dataset:

GDS1804

Platform:

GPL3500

16 Samples

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Expression profiling of cells at various time points up to 10 minutes following induction by IPTG of alternative sigma factor, sigma32, from an inducible promoter. Sigma32 mediates the heat shock response. Results identify genes regulated by sigma32.

Organism:

Escherichia coli

Type:

Expression profiling by array, log2 ratio, 2 agent, 5 time sets

Platform:

GPL3500

Series:

GSE4321

16 Samples

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(Submitter supplied) A microarray with 6400 spot features. Tip configuration: Standard 16-tip steel pin Columns per Sector: 20 Rows per Sector: 20 Column spacing: 210 um Row spacing: 210 um Protocol: PCR products for 4110 ORFs representing 95.8% of the Escherichia coli K12 MG1655 ORFs were prepared according to Richmond et al (1999; Nucleic Acids Res. 27:3821-35). The products were spotted onto glass slides to make DNA arrays as described in MGUIDE at http://cmgm.stanford.edu/pbrown/mguide/index.html and protocols on http://www.microarrays.org/

Organism:

Escherichia coli

1 DataSet

2 Series

28 Samples

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