GEO DataSets

(Submitter supplied) Dinoflagellates possess many physiological processes that appear to be under post-transcriptional control. However, the extent to which their genes are regulated post-transcriptionally remains unresolved. To gain insight into role of de novo transcription in dinoflagellates, we biosynthetically labeled RNA with 4-thiouracil to isolate newly transcribed RNA in Karenia brevis. These isolated fractions were then used for analysis of global de novo transcription by hybridization to a K. more...

Organism:

Karenia brevis

Type:

Expression profiling by array

Platform:

GPL13364

9 Samples

Download data: TXT

(Submitter supplied) Dinoflagellates possess many physiological processes that appear to be under post-transcriptional control. However, the extent to which their genes are regulated post-transcriptionally remains unresolved. To gain insight into the role of differential mRNA stability in dinoflagellates, we biosynthetically labeled RNA with 4-thiouracil to isolate newly transcribed and pre-existing RNA pools in Karenia brevis. more...

Organism:

Karenia brevis

Type:

Expression profiling by array

Platform:

GPL13364

18 Samples

Download data: TXT

(Submitter supplied) To gain an understanding of processes that underlie chronological aging in this dinoflagellate, a microarray study was carried out to identify changes in the global transcriptome that accompany the entry and maintenance of stationary phase up to the onset of cell death. The transcriptome of K. brevis was assayed using a custom 10,263 feature oligonucleotide microarray from mid-logarithmic growth to the onset of culture demise. more...

Organism:

Karenia brevis

Type:

Expression profiling by array

Platform:

GPL13364

15 Samples

Download data: TXT

(Submitter supplied) The role of coastal nutrient sources in the persistence of Karenia brevis red tides in coastal waters of Florida is currently a contentious issue that warrants investigation into the nutrient physiology of this dinoflagellate species. The molecular mechanisms by which dinoflagellates respond to nutrient availability are essentially unexplored. The current study sought to determine if the transcriptome of K. more...

Organism:

Karenia brevis

Type:

Expression profiling by array

Platform:

GPL13364

18 Samples

Download data: TXT

(Submitter supplied) The role of coastal nutrient sources in the persistence of Karenia brevis red tides in coastal waters of Florida is currently a contentious issue that warrants investigation into the nutrient physiology of this dinoflagellate species. The molecular mechanisms by which dinoflagellates respond to nutrient availability are essentially unexplored. The current study sought to determine if the transcriptome of K. more...

Organism:

Karenia brevis

Type:

Expression profiling by array

Platform:

GPL13364

18 Samples

Download data: TXT

(Submitter supplied) Dinoflagellates have evolved a nuclear organization unlike that of any other eukaryotic group. Recent studies find a predominance of post-transcriptional control of dinoflagellate gene expression. This study investigated regulation of the environmental stress response in the red tide dinoflagellate, Karenia brevis using an Agilent custom oligonucleotide microarray. K. brevis cultures were exposed to 5°C or 10°C heat shock, or three different sources of oxidative stress: 60 µM H2O2, 10 mM NaNO2, or 12 µM PbCl2 over acute time courses. more...

Organism:

Karenia brevis

Type:

Expression profiling by array

Platform:

GPL7733

30 Samples

Download data: TXT

(Submitter supplied) 60-mer oligonucleotide probes were designed from EST contigs derived from Karenia brevis cDNA libraries using Array Designer v 4.0 following Agilent's recommendations for Tm, GC content, and position. Probes were submitted to Agilent's eArray website for randomization and addition of control probes. For details on printing see Agilent's website www.agilent.com. 60-mer oligonucleotide probes were designed from EST contigs derived from Karenia brevis cDNA libraries using Array Designer v 4.0 following Agilent's recommendations for Tm, GC content, and position. more...

Organism:

Karenia brevis

5 Series

78 Samples

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(Submitter supplied) Amadid# 013772 Agilent Design File 013772_D_F_20050913 Protocol: 60-mer oligonucleotide probes were designed from EST contigs derived from Karenia brevis cDNA libraries using Array Designer v 4.0 following Agilent's recommendations for Tm, GC content, and position. Probes were submitted to Agilent's eArray website for randomization and addition of control probes. For details on printing see Agilent's website www.agilent.com.

Organism:

Karenia brevis

1 Series

30 Samples

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