txid309857[Organism:noexp] - GEO DataSets

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Items: 2

Select item 2000271411.

Detection and mapping of species and strains from the diatom genus Pseudo-nitzchia (Bacillariophyceae) along the Oregon and Washington coast using microarray-based, field-deployable, rapid, high-resolution assay

(Submitter supplied) DNA oligonucleotide microarrays were designed with 307 probes for 96 internal transcribed spacer (ITS1, located between 18S and 26S rRNA genes) sequences of known species and strains from the genus Pseudo-nitzschia (Bacillariophyceae). In addition, microarrays also carried 1893 probes targeting ITS1 aequences of marine Crenarchaeota and Alphaproteobacteria of SAR11 clade. In order to assign microarray profiles to Pseudo-nitzschia ribotypes and species and to 'train' the data analysis system, we grew cultures of Pseudo-nitzschia in the laboratory with identities confirmed through rDNA sequence analysis. more...

Organism:: Skeletonema; uncultured Pelagibacterales bacterium; environmental samples; Bradyrhizobium; Rhizobium; uncultured marine crenarchaeote; environmental samples; environmental samples; Thalassiosira; Pseudo-nitzschia; Mesorhizobium; environmental samples; Roseovarius

Type:: Genome variation profiling by array

Platform:: GPL13137
44 Samples

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Accession:: GSE27141

ID:: 200027141

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Select item 1000131372.

CombiMatrix_custom design 7327

(Submitter supplied) The custom oligonucleotide microarray is synthesized on a semiconductor using an electrochemical synthesis process. Each oligonucleotide probe (spot) is synthesized on a platinum electrode that is independently controlled by the synthesizer’s computer. Synthesis is based on established phosphoramidite chemistry and occurs at thousands of sites simultaneously according to a computer algorithm that activates only specified electrodes. more...

Organism:: Bradyrhizobium; Rhizobium; Skeletonema; Thalassiosira; Pseudo-nitzschia; Mesorhizobium; Roseovarius; uncultured marine crenarchaeote; uncultured Pelagibacterales bacterium