GEO DataSets

(Submitter supplied) Homologous meiotic recombination starts with DNA double-strand breaks (DSBs) generated by SPO11 protein. SPO11 is critical for meiosis in most species but the DSBs it makes are also dangerous because of their mutagenic and gametocidal potential, so cells must foster beneficial functions of SPO11 while minimizing its risks. SPO11 mechanism and regulation remain poorly understood. Here we report reconstitution of DNA cleavage in vitro with purified recombinant mouse SPO11 bound to its essential partner TOP6BL. more...

Organism:

Mus musculus; Cloning vector pUC19; Escherichia coli; Saccharomyces cerevisiae

Type:

Other

4 related Platforms

14 Samples

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