DNA epigenome editing using CRISPR-Cas SunTag-directed DNMT3A [RNA-Seq] - - GEO DataSets

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DNA epigenome editing using CRISPR-Cas SunTag-directed DNMT3A [RNA-Seq]

(Submitter supplied) We demonstrate that dCas9-SunTag-DNMT3A dramatically increased CpG methylation at the HOXA5 locus in human embryonic kidney 293T cells (HEK293T). Furthermore, using a single sgRNA, dCas9-SunTag-DNMT3A was able to methylate a 4.5 kb genomic region and repress HOXA5 gene expression. Reduced representation bisulfite sequencing (RRBS) and RNA-seq showed that dCas9-SunTag-DNMT3A methylated regions of interest with minimal impact on the global DNA methylome and transcriptome.

Organism:: Homo sapiens

Type:: Expression profiling by high throughput sequencing

Platform:: GPL11154
5 Samples

Download data: TXT

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Accession:: GSE97815

ID:: 200097815

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