GEO DataSets

(Submitter supplied) Effect of hsf1-R206S/F256S mutation on heat-induced transcription of genes is analyzed. Total RNA was isolated from HSF1 wild type and hsf1-R206S/F256S cells grown at 39oC for 15 min. Probe cDNA was primed by oligo(dT) and was synthesized in the presence of 33P-dCTP. Hybridization was carried out using the same GF100 GeneFilter. Value higher than 30 in HSF1 wild type cells is confident. Values from duplicate experiments were averaged, and the average fold changes were calculated. more...

Organism:

Saccharomyces cerevisiae

Type:

Expression profiling by array

Dataset:

GDS1073

Platform:

GPL205

4 Samples

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Analysis of a heat shock transcription factor 1 (HSF1) temperature sensitive mutant strain subjected to heat stress at 33 degrees C. HSF1 mutant contains an arginine to serine and a phenylalanine to serine substitution at residues 206 and 256 respectively. Results identify novel targets of HSF1.

Organism:

Saccharomyces cerevisiae

Type:

Expression profiling by array, transformed count, 2 genotype/variation sets

Platform:

GPL205

Series:

GSE2103

4 Samples

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