GEO DataSets

Analysis of NIH-3T3 embryonic fibroblasts induced to differentiate into adipocytes by overexpression of early B cell factor (EBF)-1 or peroxisome proliferator-activated receptor (PPAR)gamma2 transcription factors. Cells examined at various time points up to 10 days following overexpression.

Organism:

Mus musculus

Type:

Expression profiling by array, count, 3 agent, 4 time sets

Platform:

GPL81

Series:

GSE2192

53 Samples

Download data: CEL, EXP

(Submitter supplied) NIH-3T3 cells transduced with either EBF1-, PPARg2- or empty vector were stimulated with hormones to initiate adipocyte differentiation. RNA extraction was done using TriZol at d0, d2, d4 and d10 after stimulation. Samples were handled according to standard affymetrix protocols. Keywords = Adipogenesis Keywords = adipocyte Keywords = early B-cell factor 1 (EBF1) Keywords = commitment Keywords = differentiation Keywords = NIH-3T3 Keywords = pparg Keywords: time-course

Organism:

Mus musculus

Type:

Expression profiling by array

Dataset:

GDS1298

Platform:

GPL81

53 Samples

Download data: CEL, EXP

(Submitter supplied) Cellular differentiation is regulated through activation and repression of defined transcription factors. A hallmark of differentiation is a pronounced change in cell shape, which is determined by dynamics of the actin cytoskeleton. In de-differentiated fat (DFAT) cells and 3T3-L1 cells, we showed that treatment with the ROCK inhibitor Y-27632, by inducing remodeling of the actin cytoskelton, causes adipocyte differentiation. more...

Organism:

Mus musculus

Type:

Expression profiling by array

Platform:

GPL1261

4 Samples

Download data: CEL

(Submitter supplied) miRNA profiles of adipocyte-derived microvesicles (ADMs) on the Day 2-4 and Day 8-10 were compared.

Organism:

Mus musculus

Type:

Non-coding RNA profiling by array

Platform:

GPL7732

2 Samples

Download data: TXT

(Submitter supplied) mRNA profiles of adipocyte-derived microvesicles (ADMs) and their donor 3T3-L1 adipoyctes (Day 11) were compared. ADMs included RNA without typical 28S and 18S ribosomal RNA.

Organism:

Mus musculus

Type:

Expression profiling by array

Platform:

GPL4134

2 Samples

Download data: TXT

(Submitter supplied) The objective of this analysis was to identify the genes that are differentially expressed between preadipocytes with low or high adipogenic capability

Organism:

Mus musculus

Type:

Expression profiling by array

Platform:

GPL6246

10 Samples

Download data: CEL

(Submitter supplied) In order to identify the molecular mechanisms controlling preadipocyte commitment we derived new sublines of Swiss 3T3 fibroblasts with varying potential for adipogenesis. Swiss 2, Swiss 8, Swiss 9, and Swiss 19 differentiate into fat cells while Swiss 3, Swiss 5, and Swiss 22 have a lower propensity to differentiate into fat cells. The overall goal was to identify genes whose expression in the fibroblast state correlates with the ultimate potential of the cells to undergo adipogenesis

Organism:

Mus musculus

Type:

Expression profiling by array

Platform:

GPL1261

20 Samples

Download data: CEL

(Submitter supplied) Transcriptional profiling comparing the genes upregulated or downregulated by active hGR-Xebf3 by treating hormone DEX to those by non-active hGR-Xebf3 in Xenopus animal caps.

Organism:

Xenopus laevis

Type:

Expression profiling by array

Platform:

GPL10302

2 Samples

Download data: TXT

(Submitter supplied) Transcriptional profiling comparing the genes upregulated or downregulated by active hGR-Xebf3 by treating hormone DEX to those by non-active hGR-Xebf3 in Xenopus animal caps.

Organism:

Xenopus laevis

Type:

Expression profiling by array

Platforms:

GPL11258 GPL10302

8 Samples

Download data: TXT