GEO DataSets

(Submitter supplied) In this study, we analyzed the transcriptome of a highly differentiated mouse clonal CCD principal cell line (mpkCCD(cl4)) and the changes in the transcriptome induced by aldosterone and vasopressin. Serial analysis of gene expression (SAGE) was performed on untreated cells and on cells treated with either aldosterone or vasopressin for 4 h. The transcriptomes in these three experimental conditions were determined by sequencing 169,721 transcript tags from the corresponding SAGE libraries. more...

Organism:

Mus musculus

Type:

Expression profiling by SAGE

Platform:

GPL275

3 Samples

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(Submitter supplied) In order to identify novel gene targets of vasopressin regulation in the renal medulla, we performed a cDNA microarray study on the inner medullary tissue of mice following a 48 hour water restriction protocol. Keywords: Stress Response

Organism:

Mus musculus

Type:

Expression profiling by array

Platform:

GPL3004

12 Samples

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(Submitter supplied) Vasopressin is the major hormone that regulates renal water excretion. It does so by binding to a receptor in renal collecting duct cells, triggering signaling pathways that ultimately regulate the abundance, location, and activity of the water channel protein aquaporin 2. We took an advantage of quantitative large scale proteomic technologies and oligonucleotide microarrays to quantify steady state changes in protein and transcript abundances in response to vasopressin in a collecting duct cell line, mpkCCD clone 11 (Yu et al. more...

Organism:

Mus musculus

Type:

Expression profiling by array

Platform:

GPL1261

6 Samples

Download data: CEL, CHP

(Submitter supplied) Libraries obtained from microdissected kidney outer medullary collecting duct from C57Bl6/J mice control or after 3 days potassium-depleted diet. Keywords: other

Organism:

Mus musculus

Type:

Expression profiling by SAGE

Platform:

GPL275

2 Samples

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(Submitter supplied) Aldosterone is arguably the single most important hormone implicated in the control of blood pressure and extracellular fluid volume in mammals. It acts primarily by increasing the rate of transepithelial sodium transport in the kidney tubules. Several monogenetic defects resulting in hypertension have now been attributed to abnormalities in sodium handling within the aldosterone-sensitive distal nephron, where the epithelial sodium channel, ENaC, constitutes the rate-limiting step of sodium transport. more...

Organism:

Mus musculus

Type:

Expression profiling by array

Dataset:

GDS1707

Platform:

GPL2623

8 Samples

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(Submitter supplied) The Qiagen/Operon version 2.0 mouse oligonucleotide library, comprised of oligonucleotides (70-mers) representing 16,443 unique mouse genes, was printed on poly-L-lysine-coated glass slides. The library also included sets of positive and negative controls that were used for quality control purposes. Oligonucleotides were prepared in sets of 384-well plates at a concentration of 40 <E6>M. Arrays were prepared using a custom-built array printer equipped with Majer MicroQuill-2000 split tip pins with a capacity of 255 slides per print run. more...

Organism:

Mus musculus

1 DataSet

1 Series

8 Samples

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Analysis of kidney epithelial cortical collecting duct cells treated with aldosterone for 1 or 6 hours. Aldosterone stimulates Na+ transport in tight epithelia. Results identify candidate genes that regulate Na+ transport via the epithelial Na+ channel and mediate the early response to aldosterone.

Organism:

Mus musculus

Type:

Expression profiling by array, log2 ratio, 2 time sets

Platform:

GPL2623

Series:

GSE2933

8 Samples

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(Submitter supplied) Murine inner medullary collecting duct cells were treated for 1 hour with vehicle (control) or aldosterone. Total RNA was isolated and used as template to generate the eventual cRNA target. The experiment was repeated a total of three times. Six cRNA samples, three control and three treated, were generated and used in a total of six hybridizations. The mineralocorticoid aldosterone is a major regulator of Na+ and acid-base balance and control of blood pressure. more...

Organism:

Mus musculus

Type:

Expression profiling by array

Dataset:

GDS345

Platform:

GPL81

6 Samples

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Identification of early aldosterone-responsive genes after 1 hour exposure in inner medullary collecting duct cell line, mIMCD-3. Aldosterone is major regulator of Na(+) and acid-base balance and control of blood pressure.

Organism:

Mus musculus

Type:

Expression profiling by array, count, 2 agent sets

Platform:

GPL81

Series:

GSE434

5 Samples

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(Submitter supplied) Analysis of collecting duct response to low NaCl or high NaCl diet at the gene expression level. Results provide insight into transcriptional changes in principal and intercalated cells that occur in response to changes in dietary NaCl.

Organism:

Mus musculus

Type:

Expression profiling by high throughput sequencing

Platform:

GPL17021

6 Samples

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