GEO DataSets

(Submitter supplied) A novel porcine cDNA microarray, containing PCR products selected for embryonic expression, was used for transcriptional profiling during conceptus development. Total RNA from quadruplicate samples of small spherical (1-5mm), large spherical (6-11mm), tubular (13-45mm), and filamentous (>100mm) was extracted, amplified, and converted to 32P labeled target and hybridized to membranes. Porcine peri-implantation embryo development order is from small spherical to large spherical, then to Tubular, then to filamentous. more...

Organism:

Sus scrofa

Type:

Expression profiling by array

Dataset:

GDS844

Platform:

GPL1209

16 Samples

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Gene expression changes during peri-implantation conceptus development. Small spherical (1-5 mm), large spherical (6-11 mm), tubular (13-45 mm) and filamentous (>100 mm) embryos analyzed.

Organism:

Sus scrofa

Type:

Expression profiling by array, count, 4 development stage sets

Platform:

GPL1209

Series:

GSE1372

16 Samples

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(Submitter supplied) The objective of the present investigation was to utilize the GeneChip® Porcine Genome Array from Affymetrix possessing 20, 201 unique probe sets to identify differentially expressed genes during rapid trophoblastic elongation and attachment to the uterine surface in the pig. Identification and characterization of conceptus gene expression patterns during rapid trophoblastic elongation and attachment in the pig will provide a better understanding of the events required for successful implantation and embryonic survival.

Organism:

Sus scrofa

Type:

Expression profiling by array

Platform:

GPL3533

16 Samples

Download data: CEL

(Submitter supplied) Transcriptome profiling of in vivo-derived porcine conceptus at the tubular stage (12-80 mm) during the elongation period of peri-implantation development. Keywords: Embryo stage of development

Organism:

Sus scrofa

Type:

Expression profiling by SAGE

Platform:

GPL1270

1 Sample

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(Submitter supplied) To understand the host transcriptional response to S. enterica serovar Choleraesuis (S. Choleraesuis), the first generation Affymetrix porcine GeneChip® was used to identify differentially expressed genes in the mesenteric lymph nodes responding to infection at acute (8 hours (h), 24h, 48h post-inoculation (pi)) and chronic stages (21 days (d) pi) The objectives of this study were to identify and examine the stereotypical gene expression response within the host mesenteric lymph nodes to S. more...

Organism:

Sus scrofa

Type:

Expression profiling by array

Platform:

GPL3533

15 Samples

Download data: CEL, EXP

(Submitter supplied) Temporal changes in the embryo transcriptome between the blastocyst stage (Day 7) and initiation of elongation (Day 13) differ between in vivo- and in vitro-derived embryos and are reflective of subsequent developmental fate. The aim of this study was to examine the temporal changes in transcriptional profile as the embryo develops from a spherical blastocyst on Day 7 to an ovoid conceptus at the initiation of elongation on Day 13 and to highlight differences in these temporal gene expression dynamics between in vivo- and in vitro-derived blastocysts which may be associated with embryonic survival/mortality using the bovine Affymetrix microarray.

Organism:

Bos taurus

Type:

Expression profiling by array

Platform:

GPL2112

12 Samples

Download data: CEL

(Submitter supplied) Microarray technology permits high throughput comparisons of gene expression in different parasite stages or sexes. We now report the first use of this technology for analysis of gene expression in filarial worms. The slide array (comprised of 65 mer oligos representing 3569 EST clusters) was spotted with sequences selected from the extensive Brugia malayi EST database (http://nema.cap.ed.ac.uk/nematodeESTs/nembase.html#Annotation). more...

Organism:

Brugia malayi

Type:

Expression profiling by array

Platform:

GPL1483

8 Samples

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(Submitter supplied) A zebra mussel byssus cDNA microarray was used to identify the differentially expressed genes between attachment and detachment. Keywords: Gene differential expression

Organism:

Dreissena polymorpha

Type:

Expression profiling by array

Platform:

GPL6586

4 Samples

Download data: GPR

(Submitter supplied) 716 E.coli clones were selected from thirty-four 96-well plates and re-arranged into eight new 96-well plates. The overnight cultures from the eight 96-well plates were used to prepare the PCR templates. 10 μl of each bacterial culture was taken to mix with 90 μl sterile double distilled water. Then the mixture was incubated at 100 ℃ for 10 minutes followed by 15 minutes 1200g centrifuge. The supernatant was used as PCR templates. more...

Organism:

Dreissena polymorpha

3 Series

62 Samples

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(Submitter supplied) The anterior pituitary is comprised of five major hormone-secreting cell types which differentiate during embryonic development in a temporally distinct manner. Microarrays containing 5,128 unique cDNAs expressed in the neuroendocrine system were produced and used to identify genes with potential involvement in onset of thyroid-stimulating hormone beta-subunit, growth hormone, and prolactin gene transcription during chick embryonic development. more...

Organism:

Gallus gallus

Type:

Expression profiling by array

Dataset:

GDS1952

Platform:

GPL1744

16 Samples

Download data: TIFF

(Submitter supplied) A major research focus in our laboratory is the development of the chicken pituitary gland. As this tissue does not yield sufficient RNA to analyze using cDNA microarrays, mRNA must be amplified by reverse transcription with an oligo-dT primer containing the T7 RNA polymerase promoter site followed by in vitro transcription using T7 RNA polymerase. Therefore, this preliminary study investigating differential gene expression between embryonic day 17, when all the major cell types in the chick anterior pituitary gland have differentiated, and post-hatch day 3 was conducted to verify that amplified antisense RNA could be used in conjunction with our neuroendocrine microarrays containing 5,128 unique cDNAs to detect changes in gene expression. more...

Organism:

Gallus gallus

Type:

Expression profiling by array

Dataset:

GDS1951

Platform:

GPL1744

6 Samples

Download data: TIFF

(Submitter supplied) Spotted amplified cDNA array on glass. Produced with cDNA clones expressed in the chicken neuroendocrine system. Tissues used to produced the cDNA library included the hypothalamus, anterior pituitary gland, and pineal gland. 7200 spots Keywords = spotted Keywords = cDNA

Organism:

Gallus gallus

2 DataSets

2 Series

22 Samples

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Analysis of embryonic pituitary glands at various ages from day 10 to day 17 using a neuroendocrine system specific cDNA microarray. Results identify genes potentially involved in the onset of thyroid-stimulating hormone beta-subunit, growth hormone, and prolactin gene transcription.

Organism:

Gallus gallus

Type:

Expression profiling by array, log2 ratio, 4 age sets

Platform:

GPL1744

Series:

GSE3227

16 Samples

Download data: TIFF

Analysis of pituitary glands from day 17 embryos and post-hatch day 3 animals using a neuroendocrine system specific cDNA microarray. Major cell types in the anterior pituitary gland have differentiated at embryonic day 17. Results used to validate the method of amplifying RNA from pituitary glands.

Organism:

Gallus gallus

Type:

Expression profiling by array, log2 ratio, 2 age sets

Platform:

GPL1744

Series:

GSE3226

6 Samples

Download data: TIFF

(Submitter supplied) Prostaglandin F2 alpha (PGF2alpha) brings about regression of the bovine corpus luteum (CL). This luteolytic property of PGF2alpha is used in the beef and dairy cattle to synchronize estrus. A limitation of this protocol is an insensitivity of the early CL to luteolytic actions of PGF2alpha. The mechanisms underlying luteal sensitivity (LS) are poorly understood. The early CL has maximum number of PGF2alpha receptors; therefore differences in signaling events might be responsible for LS. more...

Organism:

Bos taurus

Type:

Expression profiling by array

Platform:

GPL3810

1 Sample

Download data: GPR

(Submitter supplied) Lung, liver, muscle (Semitendinosus), small intestine (duodenum; approximately 5 cm from stomach) were collected from six cross-bred white pigs (three 15 week old and three 17 week old). Total RNAs were isolated from these tissues to study gene expression by using the first generation of porcine long oligonucleotide microarray. In the experimental design, tissue samples from the six animals were assigned to six loops (three for younger animals and three for older animals). more...

Organism:

Sus scrofa

Type:

Expression profiling by array

Platform:

GPL1881

24 Samples

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(Submitter supplied) Zhao SH, Recknor J, Lunney JK, Nettleton D, Kuhar D, Orley S, Tuggle CK: Validation of a first-generation long-oligonucleotide microarray for transcriptional profiling in the pig.Zhao SH, Recknor J, Lunney JK, Nettleton D, Kuhar D, Orley S, Tuggle CK., Genomics. 2005 Nov;86(5):618-25. Protocol: Oligonucleotides were printed in duplicate on epoxide slides by operon. Operon probes are designed from The TIGR Gene Index Database SsGI Release 5.0 released 10/1/02. more...

Organism:

Sus scrofa

1 Series

18 Samples

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(Submitter supplied) Gene expression profile of Transgenic Adenocarcinoma of the Mouse Prostate (TRAMP) reveals murine targets for preclinical development of human prostate cancer therapy In this study, we have generated an open source TRAMP microarray dataset to identify differentially expressed genes from human prostate cancer that have concordant expression in TRAMP tumors, and thereby represent lead targets for preclinical therapy development. more...

Organism:

Mus musculus

Type:

Expression profiling by array

Platform:

GPL1261

18 Samples

Download data: CEL

(Submitter supplied) Microarrays have been widely used for the analysis of gene expression and several commercial platforms are available. The combined use of multiple platforms can overcome the inherent biases of each approach, and may represent an alternative that is complementary to RT-PCR for identification of the more robust changes in gene expression profiles. In this paper, we combined statistical and functional analysis for the cross platform validation of two oligonucleotide-based technologies, Affymetrix (AFFX) and Applied Biosystems (ABI), and for the identification of differentially expressed genes. more...

Organism:

Homo sapiens

Type:

Expression profiling by array

Platforms:

GPL570 GPL1426

12 Samples

Download data: CEL

(Submitter supplied) The maize spikelet contains two florets, each of which contains three anthers. Morphologically, the anthers in the upper and lower florets proceed through the same developmental program. To test for global differences in gene expression between these anthers and to identify genes that are coordinately regulated during development, RNA isolated from upper and lower floret anthers at six developmental stages was used to hybridize cDNA microarrays. more...

Organism:

Zea mays

Type:

Expression profiling by array

Datasets:

GDS2474 GDS2475 GDS2477

Platform:

GPL3021

144 Samples

Download data: PDF