GEO DataSets

(Submitter supplied) Identification of the genetic events that contribute to host-pathogen interactions is important for understanding the natural history of infectious diseases and developing therapeutics. Transcriptome studies conducted on pathogens have been central to this goal in recent years. However, most of these investigations have focused on specific end points or disease phases, rather than analysis of the entire time course of infection. more...

Organism:

Coxiella burnetii; Streptococcus pyogenes; Borreliella burgdorferi; Chlamydia trachomatis; Staphylococcus aureus; Yersinia pestis; Macaca fascicularis

Type:

Expression profiling by array

Platform:

GPL2129

259 Samples

Download data: CEL, EXP, TXT

(Submitter supplied) Relatively little is understood about the dynamics of global host–pathogen transcriptome changes that occur during bacterial infection of mucosal surfaces. To test the hypothesis that group A Streptococcus (GAS) infection of the oropharynx provokes a host transcriptome response, we performed genome-wide transcriptome analysis using a nonhuman primate model of experimental pharyngitis. We also identified host and pathogen biological processes and individual host and pathogen gene pairs with correlated patterns of expression, suggesting interaction. more...

Organism:

Homo sapiens; Macaca fascicularis

Type:

Expression profiling by array

Platform:

GPL96

360 Samples

Download data: CEL, CHP

(Submitter supplied) Purpose: We used dual RNA-seq to analyze the transcriptomes of serotype M1 S. pyogenes and host skeletal muscle recovered contemporaneously from infected nonhuman primates (NHPs). We identified genes important for necrotizing myositis, made isogenic deletion mutants and performed RNA-seq comparing one of those deletion mutant strains (dahA) grown in vitro and compared to its parental wild-type strain. more...

Organism:

Streptococcus pyogenes; Macaca fascicularis

Type:

Expression profiling by high throughput sequencing

4 related Platforms

54 Samples

Download data: XLSX

(Submitter supplied) Streptococcus pyogenes (Group A Strep, GAS) is a serious human pathogen with the ability to colonize mucosal surfaces such as the nasopharynx and vaginal tract, often leading to infections such as pharyngitis and vulvovaginitis. We present genome-wide RNASeq data showing the transcriptomic changes GAS undergoes during vaginal colonization. These data reveal that the regulon controlled by MtsR, a master metal regulator, is activated during vaginal colonization. more...

Organism:

Streptococcus pyogenes

Type:

Expression profiling by high throughput sequencing

Platform:

GPL20656

6 Samples

Download data: GTF, TXT

(Submitter supplied) The molecular genetic mechanisms used by bacteria to persist in humans are poorly understood. Group A Streptococcus (GAS) causes the majority of bacterial pharyngitis cases in humans and is prone to persistently inhabit the upper respiratory tract. To gain information about how GAS survives in and infects the oropharynx, we analyzed the transcriptome of a serotype M1 strain grown in saliva. The dynamic pattern of changes in transcripts of genes [spy0874/0875, herein named sptR and sptS (sptR/S), for saliva persistence] encoding a two-component gene regulatory system of unknown function suggested that SptR/S contributed to persistence of GAS in saliva. more...

Organism:

Yersinia pestis; Streptococcus sp. 'group A'; Borreliella burgdorferi; Chlamydia trachomatis; Staphylococcus aureus; Coxiella burnetii; Streptococcus pyogenes

Type:

Expression profiling by array

Platform:

GPL2129

16 Samples

Download data: CEL

(Submitter supplied) This study represents the first in vivo genome wide analysis of gene epxression of Group A Streptococcus (GAS) in humans with pharyngitis. The micorarray used is a custom microarray that relies on an electrochemical reaction as the measured signal rather than flourescence. Distinct clusters of gene expression were discovered and analyzed. A functional analysis examining differences and similarities between the clusters was performed.

Organism:

Homo sapiens; Streptococcus pyogenes SSI-1; Streptococcus pyogenes MGAS5005; Streptococcus pyogenes MGAS10750; Streptococcus pyogenes M1 GAS; Streptococcus pyogenes MGAS6180; Streptococcus pyogenes MGAS2096; Streptococcus pyogenes MGAS9429; Streptococcus pyogenes MGAS315

Type:

Expression profiling by array

Platform:

GPL10562

14 Samples

Download data: TXT

(Submitter supplied) We investigated the covS-regulation of S. pyogenes in a hypervirulent M23 strain using RNA-sequencing. The differential gene expression comparison between the covS- mutant and isogenic wild type covS+ identified altered expression of 349 (18%) genes, including a broad spectrum of virulence genes and diverse metabolic genes. The data showed that the strain achieved hypervirulence by enhancing the expression of genes responsible for invasiveness and antiphagocytosis (i.e., hasABC), by abrogating the expression of toxic genes (i.e., speB), and by compromising gene products with dispensable functions (i.e., sfb1). more...

Organism:

Streptococcus pyogenes

Type:

Expression profiling by high throughput sequencing

Platform:

GPL19982

8 Samples

Download data: XLS

(Submitter supplied) Manuscript title: The endopeptidase PepO regulates the SpeB cysteine protease and is essential for the virulence of invasive M1T1 Streptococcus pyogenes. The study investigates the effect of pepO mutation on global gene expression in GAS M1T1 strain 5448.

Organism:

Streptococcus pyogenes

Type:

Expression profiling by array

Platform:

GPL19152

3 Samples

Download data: TXT, XLSX

(Submitter supplied) JRS4 is our wild-type M6 serotype strain and JRS948 is the isogenic covR strain. Keywords: 2-channel

Organism:

Streptococcus pyogenes

Type:

Expression profiling by array

Platform:

GPL1482

16 Samples

Download data

(Submitter supplied) Two covR mutant derivatives of parental strain MGAS2221 were recovered from mice experimentally infected with MGAS2221 and shown to differ in terms of the number and concentration of secreted proteins. One of the covR mutant strains had a secretion phenotype identical to a covS mutant strain, while the other had a secretion phenotype identical to a constructed covR mutant strain. To further investigate the potential differences between the two covR mutant strains we performed expression microarray analysis.

Organism:

Chlamydia trachomatis; Streptococcus pyogenes; Borreliella burgdorferi; Yersinia pestis; Staphylococcus aureus; Coxiella burnetii

Type:

Expression profiling by array

Platform:

GPL2129

4 Samples

Download data: CEL

(Submitter supplied) The aim of this study was to determine the extent of the CcpA regulon in the M1 serotype MGAS5005. Kinkel and McIver, 2008 Infection and Immunity Keywords: group A streptococcus, CcpA

Organism:

Streptococcus pyogenes

Type:

Expression profiling by array

Platform:

GPL1482

6 Samples

Download data: GPR

(Submitter supplied) S. pyogenes strains were compared with the intact covRS form of the globally disseminated M1T1 clone to track transcriptomic changes engendered during the emergence of the M1T1 clone. The mutant covRS form of the M1T1 clone was included as a transcriptomic outlier and to provide a context for the magnitude of transcriptional shifts detected within the isolate set examined.

Organism:

Streptococcus pyogenes

Type:

Expression profiling by array

Platform:

GPL10822

36 Samples

Download data: GPR

(Submitter supplied) RNA-seq experiments of a wild-type and isogenic rocA deletion mutant serotype M28 group A streptococcus strain at two time points

Organism:

Streptococcus pyogenes

Type:

Expression profiling by high throughput sequencing

Platform:

GPL24880

12 Samples

Download data: XLSX

(Submitter supplied) Streptococcus pyogenes growth-phase dependent and Rgg-dependent transcription was analyzed Keywords: transcriptional regulation

Organism:

Streptococcus pyogenes

Type:

Expression profiling by array

Platform:

GPL3312

9 Samples

Download data

(Submitter supplied) We compared the gene expression patterns of macrophages infected with S. oralis wild type and SpxB KO, a strain that does not produce H2O2.

Organism:

Mus musculus

Type:

Expression profiling by array

Platform:

GPL21810

8 Samples

Download data: TXT

(Submitter supplied) We developed spotted oligonucleotide arrays of the S. pyogenes SF370 (an M1 serotype) genome and compared the transcriptomes of streptococci that adhere to Detroit 562 human pharyngeal cells to non-adherent (“associated”) streptococci within the same experiment. We replicated experiments independently and used dye-swaps to incorporate biological and technical variation, biological replicate experiments. more...

Organism:

Streptococcus pyogenes

Type:

Expression profiling by array

Platform:

GPL5122

8 Samples

Download data: TXT

(Submitter supplied) The goal was to perform global transcriptome analysis on isogenic wild type 2221 and FabT deletion mutant M1 strains to investigate how inactivation of the fabT gene affects gene expression.

Organism:

Streptococcus pyogenes

Type:

Expression profiling by high throughput sequencing

Platform:

GPL20656

24 Samples

Download data: XLSX

(Submitter supplied) Infection of the human host by Streptococcus pneumoniae begins with colonization of the nasopharynx, which is mediated by adherence of bacteria to respiratory epithelium. Several studies have indicated an important role for the pneumococcal capsule in this process. Here, we used microarrays to characterize the in vitro transcriptional response of human nasopharyngeal epithelial Detroit 562 cells to adherence of serotype 2-encapsulated strain D39, serotype 19F-encapsulated strain G54, serotype 4-encapsulated strain TIGR4, and their nonencapsulated derivatives (Δcps). more...

Organism:

Homo sapiens

Type:

Expression profiling by array

Dataset:

GDS3041

Platform:

GPL570

32 Samples

Download data: CEL, EXP

Analysis of pharyngeal epithelial cells exposed to various encapsulated strains of Streptococcus pneumoniae. Nasopharynx colonization by S. pneumoniae is mediated by adherence to the respiratory epithelium. Results provide insight into the capsule-dependent host response to pneumococcal adherence.

Organism:

Homo sapiens

Type:

Expression profiling by array, count, 5 agent, 3 cell type, 3 dose, 2 infection sets

Platform:

GPL570

Series:

GSE8527

32 Samples

Download data: CEL, EXP

(Submitter supplied) The rivR gene encodes a putative transcriptional regulator, while the rivX gene encodes a putative small regulatory RNA. To determine the RivRX regulon during exponential phase growth in THY broth we compared parental strain MGAS2221 with isogenic rivRX mutant strain 2221ΔrivRX.

Organism:

Streptococcus pyogenes

Type:

Expression profiling by array

Platform:

GPL9080

4 Samples

Download data: CEL