GEO DataSets

(Submitter supplied) This is the first use of cDNA microarrays to study the influence of GH transgenesis on liver gene expression in a non-mammalian vertebrate. Three groups of salmon were examined: transgenic on full ration (T), transgenic on restricted ration (R), and control nontransgenic (C). Daily weight gains in T were approximately 8-fold higher than in C, and 4-fold higher than in R. Differential gene expression in T, R, and C samples was determined using ~ 16,000 gene microarrays. more...

Organism:

Salmo salar; Oncorhynchus kisutch

Type:

Expression profiling by array

Platform:

GPL2989

6 Samples

Download data

(Submitter supplied) The influence of GH transgenesis on liver gene expression in coho salmon was examined. Gene expression in livers of transgenic salmon on a restricted ration (R) was compared to that in livers of nontransgenic control salmon (C). Keywords: Transcript profile

Organism:

Salmo salar

Type:

Expression profiling by array

Platform:

GPL2844

8 Samples

Download data

(Submitter supplied) Coho Salmon GH Transgenic Study Keywords: other

Organism:

Salmo salar; Oncorhynchus kisutch; Oncorhynchus mykiss

Type:

Expression profiling by array

Platform:

GPL966

9 Samples

Download data: TIFF

(Submitter supplied) By directly comparing gene expression in wild type, domestic, and GH transgenic strains of salmon, we have found that domestication and GH transgenesis are modifying similar genetic pathways. Genes in many different physiological pathways show modified expression in domestic and GH transgenic strains relative to wild-type, but effects are strongly correlated. Genes specifically involved in growth regulation (IGF-I, GHR, IGF-II, THR) are also concordantly regulated in domestic and transgenic fish, and both strains show elevated levels of circulating IGF-I. more...

Organism:

Oncorhynchus kisutch; Oncorhynchus mykiss; Oncorhynchus tshawytscha; Osmerus mordax; Salmo salar; Coregonus clupeaformis

Type:

Expression profiling by array

Platform:

GPL2716

29 Samples

Download data: TXT

(Submitter supplied) Although studies have established that exogenous growth hormone (GH) treatment stimulates growth in fish, its effects on target tissue gene expression are not well characterized. We assessed the effects Posilac® (Monsanto Co., St. Louis, MO), a recombinant bovine somatotropin, on tissue transcript levels. Transcript abundance was measure in liver and muscle using the GRASP 16 K cDNA microarray. A selection of the genes identified as altered with the microarray, and also transcripts for insulin-like growth factors, growth hormone receptors (GHR) and myostatins were measured by realtime PCR in the liver, muscle, brain, kidney, intestine, stomach, gill and heart. more...

Organism:

Oncorhynchus mykiss; Salmo salar

Type:

Expression profiling by array

Platform:

GPL3976

16 Samples

Download data: TXT

(Submitter supplied) Growth rate can be genetically modified in many vertebrates by domestication and selection, and more recently by transgenesis overexpressing growth factor genes (e.g. growth hormone, GH). While the phenotypic end consequence is similar, it is currently not clear whether the same modifications to physiological pathways are occurring in both genetic processes, nor to what extent they may interact when combined. more...

Organism:

Salmo salar; Coregonus clupeaformis; Osmerus mordax; Oncorhynchus mykiss; Oncorhynchus tshawytscha

Type:

Expression profiling by array

Platform:

GPL2716

30 Samples

Download data: TXT

(Submitter supplied) Microarrays have been widely used for the analysis of gene expression and several commercial platforms are available. The combined use of multiple platforms can overcome the inherent biases of each approach, and may represent an alternative that is complementary to RT-PCR for identification of the more robust changes in gene expression profiles. In this paper, we combined statistical and functional analysis for the cross platform validation of two oligonucleotide-based technologies, Affymetrix (AFFX) and Applied Biosystems (ABI), and for the identification of differentially expressed genes. more...

Organism:

Homo sapiens

Type:

Expression profiling by array

Platforms:

GPL570 GPL1426

12 Samples

Download data: CEL

(Submitter supplied) To determine the transcriptional response to Salmonella enterica serovar Enteritidis (SE) infection, a newly developed chicken 44K Agilent array was used to analyze total RNA of heterophils from SE-resistant (line A) and SE–susceptible chickens (line B), treated with in vitro infection of SE (I) or non SE medium (N) for 1 hr. A dual-color balanced design was used to provide a direct comparison between SE-infected and non-infected groups (AI/AN, BI/BN) and between line A and line B (AN/BN, AI/BI). more...

Organism:

Gallus gallus

Type:

Expression profiling by array

Platform:

GPL4993

16 Samples

Download data: GPR

(Submitter supplied) d-serine is naturally present throughout the human body. It is also used as add-on therapy for treatment-refractory schizophrenia. d-Serine interacts with the strychnine-insensitive glycine binding site of NMDA receptor, and this interaction could lead to potentially toxic activity (i.e., excitotoxicity) in brain tissue. The transcriptomic changes that occur in the brain after d-serine exposure have not been fully explored. more...

Organism:

Rattus norvegicus

Type:

Expression profiling by array

Dataset:

GDS3643

Platform:

GPL1355

24 Samples

Download data: CEL

Analysis of forebrains of animals treated with up to 500 mg/kg D-serine for 96 hours. D-serine is involved in many physiological processes through its interaction with the glycine binding site of the NMDA receptor. Results provide insight into the impact of D-serine exposure on neuronal functions.

Organism:

Rattus norvegicus

Type:

Expression profiling by array, count, 2 agent, 6 dose sets

Platform:

GPL1355

Series:

GSE10748

24 Samples

Download data: CEL

(Submitter supplied) Aim of present study was to describe the changes induced deletion of the Wfs1 gene in the temporal lobe of mice. Mutant mice were backcrossed to two different genomic backgrounds in order to exclude confounding foreign genomic background influence. Samples from temporal lobes were analyzed by using Affymetrix Genechips, expression profiles were functionally annotated by using GSEA and Ingenuity Pathway Analysis. more...

Organism:

Mus musculus

Type:

Expression profiling by array

Dataset:

GDS3647

Platform:

GPL1261

37 Samples

Download data: CEL

Analysis of temporal lobes of C57B6 and 129S6 animals deficient for the Wfs1 protein. WFS1 gene mutations cause the Wolfram syndrome, an autosomal recessive disorder characterized by diabetes insipidus, diabetes mellitus, optic atrophy, and deafness. WFS1 is expressed in the temporal lobe.

Organism:

Mus musculus

Type:

Expression profiling by array, transformed count, 2 genotype/variation, 2 strain sets

Platform:

GPL1261

Series:

GSE15293

37 Samples

Download data: CEL

(Submitter supplied) Muscle atrophy is a physiological response to diverse physiological and pathological conditions that trigger muscle deterioration through distinct cellular stimuli. Despite different physiological signals, the resulting biochemical changes in atrophying muscle share many common cascades. Muscle deterioration as a physiological response to the energetic demands of fish spawning represents a unique model for studying the mechanisms of muscle degradation in non-mammalian animals. more...

Organism:

Salmo salar; Oncorhynchus mykiss

Type:

Expression profiling by array

Platform:

GPL2899

5 Samples

Download data

(Submitter supplied) The study was aimed at examining the effect of the model organic oxygen reactive species (ROS) tert-Butyl Hydroperoxide (tBHP) on hepatic multi-gene expression patterns of the fish Lithognathus mormyrus. Fish were exposed to injected tBHP followed by extraction of RNA from their livers. The corresponding labeled cDNAs of treated versus control fish were applied onto a cDNA microarray of ~1500 unique sequences and differentially expressed genes were identified. more...

Organism:

Lithognathus mormyrus

Type:

Expression profiling by array

Platform:

GPL5351

5 Samples

Download data: GPR, TXT

(Submitter supplied) A series of gene expression measurements of normal myometrium and uterine fibroids with mutated or wild-type fumarate hydratase (FH) gene. Keywords: other

Organism:

Homo sapiens

Type:

Expression profiling by array

Dataset:

GDS2246

Platform:

GPL96

10 Samples

Download data: CEL, EXP, RPT

(Submitter supplied) A series of gene expression measurements of uterine fibroids with mutated fumarate hydratase (FH) gene and normal myometrium. Keywords: other

Organism:

Homo sapiens

Type:

Expression profiling by array

Dataset:

GDS2245

Platform:

GPL96

18 Samples

Download data: CEL, EXP, RPT

(Submitter supplied) A series of gene expression measurements of uterine fibroids with mutated or wild-type fumarate hydratase (FH) gene. Keywords: other

Organism:

Homo sapiens

Type:

Expression profiling by array

Dataset:

GDS1476

Platform:

GPL96

22 Samples

Download data: CEL, EXP, RPT

Analysis of uterine fibroids with mutations in the fumarate hydratase (FH) gene. FH catalyzes the hydration of fumarate in the TCA cycle. Heterozygous germline mutations in FH cause uterine fibroids and renal cell cancer. Results provide insight into the pathogenesis of TCA cycle-related tumors.

Organism:

Homo sapiens

Type:

Expression profiling by array, count, 2 disease state, 3 genotype/variation sets

Platform:

GPL96

Series:

GSE2725

10 Samples

Download data: CEL, EXP, RPT

Analysis of uterine fibroids with mutations in the fumarate hydratase (FH) gene. FH catalyzes the hydration of fumarate in the TCA cycle. Heterozygous germline mutations in FH cause uterine fibroids and renal cell cancer. Results provide insight into the pathogenesis of TCA cycle-related tumors.

Organism:

Homo sapiens

Type:

Expression profiling by array, count, 2 disease state, 2 genotype/variation, 11 individual sets

Platform:

GPL96

Series:

GSE2724

18 Samples

Download data: CEL, EXP, RPT

Analysis of 7 uterine fibroids carrying fumarate hydratase (FH) mutations. FH catalyzes hydration of fumarate in the Krebs tricarboxylic acid cycle. Results provide insight into the connection between the disruption of mitochondrial metabolic pathways and neoplasia.

Organism:

Homo sapiens

Type:

Expression profiling by array, count, 2 genotype/variation sets

Platform:

GPL96

Series:

GSE2152

22 Samples

Download data: CEL, EXP, RPT