GEO DataSets

(Submitter supplied) Adult male fish were exposed to the various temperature acclimation conditions as outlined in the published manuscript and RNA was extracted from fish at each time point as listed in the experiment set description. Abstract: Eurythermal ectotherms commonly thrive in environments that expose them to large variations in temperature on daily and seasonal bases. The roles played by alterations in gene expression in enabling eurytherms to adjust to these two temporally distinct patterns of thermal stress are poorly understood. more...

Organism:

Austrofundulus limnaeus

Type:

Expression profiling by array

Platform:

GPL3311

65 Samples

Download data

(Submitter supplied) The goal of this study was to identify transcriptional resonses to acute heat stress following differential acclimation in the highly eurythermal goby fish, Gillichthys mirabilis. G. mirabilis were acclimated to 3 temperatures (9C, 19C and 28C) for one month and then exposed to an acute heat ramp at 4C/hr and sampled during the timecourse. Gill tissues were dissected and immediately flash frozen in liquid nitrogen. more...

Organism:

Gillichthys mirabilis

Type:

Expression profiling by array

Platform:

GPL10169

62 Samples

Download data: GPR

(Submitter supplied) Subspecies of the Atlantic killifish, Fundulus heteroclitus, differ in their maximum thermal tolerance. To determine whether there is a link between the heat shock response (HSR) and maximum thermal tolerance, we exposed 20ºC acclimated killifish from these subspecies to a 2hr heat shock at 34ºC and examined gene expression during heat shock and recovery using real time quantitative PCR and a heterologous cDNA microarray designed for salmonid fishes. more...

Organism:

Salmo salar; Fundulus heteroclitus; Coregonus clupeaformis; Oncorhynchus mykiss; Oncorhynchus tshawytscha; Osmerus mordax

Type:

Expression profiling by array

Platform:

GPL2716

16 Samples

Download data: MEV

(Submitter supplied) We used microarrays to investigate the transcriptome of 6 days old male flies exposed to either 15 or 25 C development at either constant or fluctuating temperatures. Further, we investigated gene expression at benign (20C) and high (35C) temperatures With global climate change temperature means and variability are expected to increase. Thus, exposures to elevated temperatures are expected to become an increasing challenge for terrestrial ectotherm populations. more...

Organism:

Drosophila melanogaster

Type:

Expression profiling by array

Platform:

GPL1322

24 Samples

Download data: CEL

(Submitter supplied) The Atlantic killifish (Fundulus heteroclitus), native to estuarine areas of the Atlantic coast of the United States, has become a valuable ecotoxicological model due to its ability to acclimate to rapid environmental changes and adapt to polluted habitats. Killifish respond to rapid increases in salinity with an immediate change in gene expression, as well as long-term remodeling of the gills. Arsenic, a major environmental toxicant, was previously shown to inhibit the ability of killifish gill to respond to a rapid increase in salinity. more...

Organism:

Fundulus heteroclitus

Type:

Non-coding RNA profiling by high throughput sequencing

Platform:

GPL25194

24 Samples

Download data: FA, TXT

(Submitter supplied) To investigate the molecular basis of fluctuating temperature induced phenotypic plasticity, we ran genome-wide transcriptomic analysis on Drosophila melanogaster subjected to acclimation at constant (19 +/- 0 degree Celcius) and fluctuating (19 +/- 8 degree Celcius) temperatures and contrasted the induction of molecular mechanisms in adult males, adult females, and larvae. We investigated whether fluctuations act by permanently activating the involved mechanisms, or whether fluctuations repeatedly activate and repress mechanisms, during the hot (or heating) and the cold (or cooling) phase of thermal fluctuations. more...

Organism:

Drosophila melanogaster

Type:

Expression profiling by high throughput sequencing

Platform:

GPL23702

80 Samples

Download data: CSV

(Submitter supplied) Changes in environmental temperature can profoundly change species habitats and result in populations facing suboptimal environments. Many aquatic organisms are restricted in terms of migration by their habitat requirements. Also due to anthropogenic migration barriers (both physical as well as chemical), organisms are often left with no choice but to acclimate (or, in the long run, adapt) to their changing environment. more...

Organism:

Danio rerio

Type:

Expression profiling by array

Platform:

GPL9074

36 Samples

Download data: GPR

(Submitter supplied) Intertidal zone organisms can experience transient freezing temperatures during winter low tides, but their extreme cold tolerance mechanisms are not known. Petrolisthes cinctipes is a temperate mid-high intertidal zone crab species that can experience wintertime habitat temperatures below the freezing point of seawater. We examined how cold tolerance changed during the initial phase of thermal acclimation to cold and warm temperatures, as well as the persistence of cold tolerance during long-term thermal acclimation. more...

Organism:

Petrolisthes cinctipes

Type:

Expression profiling by array

Platform:

GPL15157

50 Samples

Download data: GPR

(Submitter supplied) Arctic Mesorhizobium strain N33 was isolated from nodules of the Oxytropis arctobia in Canada’s eastern Arctic. This symbiotic bacterium can grow from 0 to 30°C, is one of the best known cold-adapted rhizobia, and can fix nitrogen at ~10°C. Here, the key molecular mechanisms of cold adaptation were investigated by determining changes in transcript profiles when cells were treated under eight different temperature conditions, including both sustained and transient cold treatments compared with cells grown at room temperature.

Organism:

Mesorhizobium sp. N33

Type:

Expression profiling by array

Platform:

GPL19113

42 Samples

Download data: GPR

(Submitter supplied) In this study, we examined the transcriptomic responses to temperature acclimation (14oC, 20oC, and 25oC) in atrial and ventricular tissues of Pacific bluefin tuna (PBFT). A global gene expression analysis using a bluefin tuna-specific microarray indicated profound changes in expression of genes associated with energy metabolism, protein turnover, cellular stress response, oxidative stress and apoptosis. more...

Organism:

Thunnus orientalis; Thunnus thynnus

Type:

Expression profiling by array

Platform:

GPL15374

36 Samples

Download data: TXT

(Submitter supplied) We exposed adult delta smelt to varying levels of sublethal thermal stress to quantify the genes involved in their cellular stress response and identify sublethal stress thresholds

Organism:

Hypomesus transpacificus

Type:

Expression profiling by array

Platform:

GPL19926

49 Samples

Download data: TXT

(Submitter supplied) Long-term exposure of Atlantic salmon to 19°C resulted in cardiac gene and protein expression changes indicating that the unfolded protein response, vascularization, remodeling of connective tissue and altered innate immune responses were part of the cardiac acclimation or response to elevated temperature

Organism:

Oncorhynchus mykiss; Salmo salar

Type:

Expression profiling by array

Platform:

GPL6154

2 Samples

Download data: GPR

(Submitter supplied) In this study we use nimblegen high-density arrays to examine gene expression regulation in a common-garden experiment varying thermal environments. We report genome-wide patterns of gene expression in two heat tolerant southern and two heat-sensitive northern clones of Daphnia pulex exposed to either optimal (18°C) or substressful (28°C) temperatures.

Organism:

Daphnia pulex

Type:

Expression profiling by array

Platform:

GPL11278

36 Samples

Download data: PAIR, TXT

(Submitter supplied) The global transcriptional response of Saccharomyces cerevisiae was investigated in low temperature chemostat cultures grown in carbon or nitrogen limitation. During steady state chemostats, the growth rates and in vivo fluxes were kept constant however the growth-limiting nutrient was significantly higher at 12oC than at 30oC and had significant effects on transcriptional responses. Growth at 12oC resulted in a rearrangement of transporters for the limiting nutrient, where hexose transporters (HXTs) and ammonium permeases (MEPs) were differentially expressed in cultures grown at 30oC in carbon and nitrogen limitations, respectively. more...

Organism:

Saccharomyces cerevisiae

Type:

Expression profiling by array

Platform:

GPL90

12 Samples

Download data: CEL, EXP

(Submitter supplied) Liver plays a key role during the stress adaptation process. Therefore to understand the molecular adjustments that occur in fish to counter the stress during transport, transcriptional changes in zebrafish liver were analyzed with a 4x44K oligo array using total RNA extracted from fish prior to transport (basal), immediately after packing (0 h) and during the mock transport (48 and 72 h).

Organism:

Danio rerio

Type:

Expression profiling by array

Platform:

GPL14664

16 Samples

Download data: TXT

(Submitter supplied) Background: Ocean temperatures are projected to increase over the coming century, with dramatic consequences for the marine biosphere. Diatoms are important contributors to marine primary production and the ocean carbon cycle, yet the molecular mechanisms that regulate their acclimation and adaptation to temperature are poorly understood. Method: Here we use a transcriptomic approach to identify the molecular mechanisms associated with temperature acclimation and adaptation in closely related colder- and warmer-adapted diatom species. more...

Organism:

Chaetoceros sp.

Type:

Expression profiling by high throughput sequencing

Platform:

GPL26060

18 Samples

Download data: CSV

(Submitter supplied) Rainbow smelt (Osmerus mordax) are freeze-resistant fish that accumulate glycerol and produce an antifreeze protein during winter. Quantitative reverse transcription-PCR (qPCR) and subtractive hybridization studies have revealed a few genes in smelt liver to be differentially regulated in winter in comparison with the fall when water temperatures are warmer. In order to further define the suite of processes that are regulated seasonally, we undertook a large-scale analysis of gene expression by hybridization of smelt cDNA to the salmonid 16K cGRASP cDNA microarray. more...

Organism:

Salmo salar; Osmerus mordax

Type:

Expression profiling by array

Platform:

GPL6260

3 Samples

Download data: TXT, XLS

(Submitter supplied) Transcriptional profiling of gene responses in liver in the coral reef fish Pomacentrus moluccensis in response to different types of environmental stress: cold, heat, hypoxic and hyposmotic shock. Goal was to determine the common effects of different types of environmental stress on gene expression as well as responses unique to different stressors. Abstract from Kassahn et al. BMC Genomics (2007) 8:358 Background While our understanding of the importance of transcriptional regulation for biological function is continuously growing, we still know comparatively little about how environmentally-induced stress affects gene expression in vertebrates and how consistent transcriptional stress responses are across different types of environmental stress. more...

Organism:

Danio rerio; Pomacentrus moluccensis

Type:

Expression profiling by array

Platform:

GPL3365

59 Samples

Download data: SPOT, TIFF

(Submitter supplied) These samples are from RNA collected from specimens held at 4 temperatures (15.5, 20.0, 25.0 and 28.6degC). Specimens were ramped to one of those temperatures over 4 days following a common garden at 11degC for two weeks. RNA was extracted from thorax tissue and used for RNA-seq. Genes with statistically different expression levels among temperatures were identified.

Organism:

Dicosmoecus gilvipes

Type:

Expression profiling by high throughput sequencing

Platform:

GPL32363

20 Samples

Download data: CSV, TXT

(Submitter supplied) see Gracey AY, Fraser EJ, Li W, Fang Y, Taylor RR, Rogers J, Brass A, and Cossins AR. Coping with cold: An integrative, multitissue analysis of the transcriptome of a poikilothermic vertebrate. Proc Natl Acad Sci USA 101:16970-5, 2004.

Organism:

Carassius auratus; Cyprinus carpio

12 Series

51 Samples

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