GEO DataSets

(Submitter supplied) Microarray-based expression profiling of dissected gonads from mes-4 mutants reveals that MES-4 is required to repress expression of a set of X-linked genes. Keywords: Mutant analysis of mes-4 in dissected C. elegans gonads

Organism:

Caenorhabditis elegans

Type:

Expression profiling by array

Platform:

GPL3860

4 Samples

Download data

(Submitter supplied) Determination of gene expression levels in wild-type early embryo. First published in Rechtsteiner et al. 2010 PLoS Genetics.

Organism:

Caenorhabditis elegans

Type:

Expression profiling by array

Platform:

GPL18856

4 Samples

Download data: PAIR, TXT

(Submitter supplied) We asked if the perinuclear position of chromosome arms in C. elegans depends on the histone methyltransferases MET-2 and SET-25. To this end, we performed LMN-1-DamID in wild-type (N2) and mutant (set-25 met-2) strains. LMN-1-DamID signal on chromosome arms was significantly reduced in the mutant.

Organism:

Caenorhabditis elegans

Type:

Other

Platform:

GPL8134

6 Samples

Download data: PAIR, TAB

(Submitter supplied) Here we uncover antagonistic regulation of transcript levels in the germline of Caenorhabditis elegans hermaphrodites. The histone methyltransferase MES-4 marks genes expressed in the germline with methylated Lys36 on histone H3 (H3K36me) and promotes their transcription; MES-4 also represses genes normally expressed in somatic cells and genes on the X chromosomes. The DRM complex, which includes E2F/DP and Retinoblastoma homologs, affects germline gene expression and prevents excessive repression of X-chromosome genes. more...

Organism:

Caenorhabditis elegans

Type:

Expression profiling by array

Platform:

GPL200

12 Samples

Download data: CEL

(Submitter supplied) ChIP-chip of Mes-4, H3K36me3 and H3K27me3 in N2 C. elegans early embryo

Organism:

Caenorhabditis elegans

Type:

Genome binding/occupancy profiling by genome tiling array

Platform:

GPL8647

8 Samples

Download data: PAIR

(Submitter supplied) This SuperSeries is composed of the SubSeries listed below.

Organism:

Caenorhabditis elegans

Type:

Expression profiling by array; Genome binding/occupancy profiling by genome tiling array

Platforms:

GPL5883 GPL8647

25 Samples

Download data: GPR, PAIR

(Submitter supplied) ChIP-chip of MES-4, H3K36me3 and H3K27me3 in mes-4 RNAi C. elegans early embryo

Organism:

Caenorhabditis elegans

Type:

Genome binding/occupancy profiling by genome tiling array

Platform:

GPL8647

5 Samples

Download data: PAIR

(Submitter supplied) Trascriptional profiling of C. elegans adult germ lines comparing mes-2(bn11)unc-4(e120) mutants and unc-4(e120) (wild type), mes-4(bn85) mutants and N2 (wild type), mes-2(bn11)unc-4(e120); mes-4(bn85) and unc-4(e120) (wild type) at 20 degrees.

Organism:

Caenorhabditis elegans

Type:

Expression profiling by array

Platform:

GPL5883

12 Samples

Download data: GPR

(Submitter supplied) During meiosis, gene expression is silenced in aberrantly unsynapsed chromatin and in heterogametic sex chromosomes. Initiation of sex chromosome silencing is disrupted in meiocytes with sex chromosome-autosome translocations. To determine whether this is due to aberrant synapsis or loss of continuity of sex chromosomes, we engineered Caenorhabditis elegans with non-translocated, bisected X chromosomes. more...

Organism:

Caenorhabditis elegans

Type:

Expression profiling by high throughput sequencing

Platform:

GPL19757

2 Samples

Download data: XLSX

(Submitter supplied) This SuperSeries is composed of the SubSeries listed below.

Organism:

Caenorhabditis elegans

Type:

Genome binding/occupancy profiling by high throughput sequencing; Expression profiling by high throughput sequencing

Platform:

GPL13657

40 Samples

Download data

(Submitter supplied) Germline nuclear RNAi in C. elegans is a transgenerational gene-silencing pathway that leads to the H3K9 trimethylation (H3K9me3) response and transcriptional repression of target genes. The H3K9me3 response induced either by exogenous dsRNA or endogenous siRNA (endo-siRNA) is highly specific to the target loci and transgenerationally heritable. Despite these features, the role of H3K9me3 in transcriptional repression and heritable gene silencing at native target genes has not been tested. more...

Organism:

Caenorhabditis elegans

Type:

Expression profiling by high throughput sequencing

Platform:

GPL13657

5 Samples

Download data: XLSX

(Submitter supplied) Germline nuclear RNAi in C. elegans is a transgenerational gene-silencing pathway that leads to the H3K9 trimethylation (H3K9me3) response and transcriptional repression of target genes. The H3K9me3 response induced either by exogenous dsRNA or endogenous siRNA (endo-siRNA) is highly specific to the target loci and transgenerationally heritable. Despite these features, the role of H3K9me3 in transcriptional repression and heritable gene silencing at native target genes has not been tested. more...

Organism:

Caenorhabditis elegans

Type:

Expression profiling by high throughput sequencing

Platform:

GPL13657

5 Samples

Download data: XLSX

(Submitter supplied) Germline nuclear RNAi in C. elegans is a transgenerational gene-silencing pathway that leads to the H3K9 trimethylation (H3K9me3) response and transcriptional repression of target genes. The H3K9me3 response induced either by exogenous dsRNA or endogenous siRNA (endo-siRNA) is highly specific to the target loci and transgenerationally heritable. Despite these features, the role of H3K9me3 in transcriptional repression and heritable gene silencing at native target genes has not been tested. more...

Organism:

Caenorhabditis elegans

Type:

Genome binding/occupancy profiling by high throughput sequencing

Platform:

GPL13657

30 Samples

Download data: XLSX

(Submitter supplied) The plasticity of ageing suggests that longevity may be controlled epigenetically by specific alterations in chromatin state. The link between chromatin and ageing has mostly focused on histone deacetylation by the Sir2 family1, 2, but less is known about the role of other histone modifications in longevity. Histone methylation has a crucial role in development and in maintaining stem cell pluripotency in mammals3. more...

Organism:

Caenorhabditis elegans

Type:

Expression profiling by array

Datasets:

GDS4575 GDS5012

Platform:

GPL200

23 Samples

Download data: CEL

Analysis of day 8 germline-deficient glp-1 mutants treated with ash-2 RNAi. The ASH-2 trithorax complex trimethylates histone H3 at lysine 4 (H3K4); an intact germline is required for ASH-2-induced lifespan extension. Results provide insight into the role of histone methylation in longevity.

Organism:

Caenorhabditis elegans

Type:

Expression profiling by array, transformed count, 2 genotype/variation, 2 protocol sets

Platform:

GPL200

Series:

GSE30505

11 Samples

Download data: CEL

Analysis of day 2 germline-deficient glp-1 mutants treated with ash-2 RNAi. The ASH-2 trithorax complex trimethylates histone H3 at lysine 4 (H3K4); an intact germline is required for ASH-2-induced lifespan extension. Results provide insight into the role of histone methylation in longevity.

Organism:

Caenorhabditis elegans

Type:

Expression profiling by array, transformed count, 2 genotype/variation, 2 protocol sets

Platform:

GPL200

Series:

GSE30505

12 Samples

Download data: CEL

(Submitter supplied) Maternally synthesized products play critical roles in development of offspring. A premier example is the C. elegans H3K36 methyltransferase MES-4, which is essential for germline survival and development in offspring. How maternal MES-4 protects the germline is not well understood, but its role in H3K36 methylation hinted that it may regulate gene expression in Primordial Germ Cells (PGCs). We tested this hypothesis by profiling transcripts from nascent germlines (PGCs and their descendants) dissected from wild-type and mes-4 mutant (lacking maternal and zygotic MES-4) larvae. more...

Organism:

Caenorhabditis elegans

Type:

Expression profiling by high throughput sequencing

Platforms:

GPL18245 GPL26672

132 Samples

Download data: TXT

(Submitter supplied) This SuperSeries is composed of the SubSeries listed below.

Organism:

Caenorhabditis elegans

Type:

Genome binding/occupancy profiling by high throughput sequencing; Expression profiling by high throughput sequencing; Non-coding RNA profiling by high throughput sequencing

Platform:

GPL18245

99 Samples

Download data

(Submitter supplied) The establishment and maintenance of chromatin domains shape the epigenetic memory of a cell, with histone H3 lysine 9 methylation defining repressed heterochromatin. We show that in C. elegans the SET-25 (SUV39/G9a) HMT that catalyzes H3K9me1-3, is able to establish repressed domains de novo. We identify here two distinct pathways that recruit SET-25 to its targets. One requires LIN-61 (L3MBTL2), a conserved protein with 4 MBT domains that recognizes H3K9me2 deposited by the HMT MET-2 (SETDB1). more...

Organism:

Caenorhabditis elegans

Type:

Non-coding RNA profiling by high throughput sequencing

Platform:

GPL18245

18 Samples

Download data: TAB

(Submitter supplied) The establishment and maintenance of chromatin domains shape the epigenetic memory of a cell, with histone H3 lysine 9 methylation defining repressed heterochromatin. We show that in C. elegans the SET-25 (SUV39/G9a) HMT that catalyzes H3K9me1-3, is able to establish repressed domains de novo. We identify here two distinct pathways that recruit SET-25 to its targets. One requires LIN-61 (L3MBTL2), a conserved protein with 4 MBT domains that recognizes H3K9me2 deposited by the HMT MET-2 (SETDB1). more...

Organism:

Caenorhabditis elegans

Type:

Expression profiling by high throughput sequencing

Platform:

GPL18245

21 Samples

Download data: TAB