GEO DataSets

(Submitter supplied) Expression profiling, ChiP-CHIP and phenotypic analysis were used to investigate the functional relationships of class III NAD+-dependent HDACs (Sirtuins) in fission yeast. We detected significant histone acetylation increases in Sirtuin mutants at their specific genomic binding targets and were thus able to identify an in vivo substrate preference for each Sirtuin. At heterochromatic loci, we demonstrate that although Hst2 is mainly cytoplasmic, a nuclear pool of Hst2 co-localises with the other Sirtuins at silent regions (cen, mat, tel, rDNA) and that like the other Sirtuins, Hst2 is required for rDNA and centromeric silencing. more...

Organism:

Schizosaccharomyces pombe

Type:

Expression profiling by array; Genome binding/occupancy profiling by genome tiling array

6 related Platforms

77 Samples

Download data: TXT

(Submitter supplied) Expression profiling of mutant cells compared to wild-type cells. Expression profiling of S. pombe CENP-B homolog cbp1∆ (abp1/SPBC1105.04c) and histone deacetylase double mutant clr3∆ clr6-1 (SPBC800.03 SPBC36.05c) cells.

Organism:

Schizosaccharomyces pombe

Type:

Expression profiling by array

Platform:

GPL6503

4 Samples

Download data: TXT

(Submitter supplied) Genome wide roles for fission yeast HDACs Keywords: Genome wide roles for fission yeast HDACs

Organism:

Schizosaccharomyces pombe

Type:

Genome binding/occupancy profiling by genome tiling array

11 related Platforms

127 Samples

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(Submitter supplied) Tiling ChIP-CHIP Ssn6-GFP, Tp11-GFP, Tup12-GFP and Hst4 vs WT Expression

Organism:

Schizosaccharomyces pombe

Type:

Genome binding/occupancy profiling by genome tiling array

Platforms:

GPL2740 GPL2738 GPL2862

6 Samples

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(Submitter supplied) We have performed a genome wide expression profile study of the transcriptional co-repressor proteins Ssn6 in Schizosaccharomyces pombe. The expression of a conditional Ssn6HA-ts strain was compared against the expression in a WT strain as described before (Xue et al, 2004), except that cells were shifted to 36 deg 1 h prior to RNA extraction. Keywords: Conditional mutant

Organism:

Schizosaccharomyces pombe

Type:

Expression profiling by array

Platform:

GPL3451

2 Samples

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(Submitter supplied) This SuperSeries is composed of the SubSeries listed below.

Organism:

Schizosaccharomyces pombe

Type:

Expression profiling by array; Genome binding/occupancy profiling by array; Genome binding/occupancy profiling by genome tiling array

7 related Platforms

20 Samples

Download data: TXT

(Submitter supplied) We have performed a genome wide investigation for the binding locations of the transcriptional co-repressor proteins Ssn6, Tup11 and Tup12 in the fission yeast Schizosaccharomyces pombe. We have used a ChIP protocol described previously (Robyr et al, 2003) with microarrays containing ORF and IGR fragments representing the complete fission yeast genome (Wiren et al, 2005). Keywords: ChIP-CHIP

Organism:

Schizosaccharomyces pombe

Type:

Genome binding/occupancy profiling by genome tiling array

Platform:

GPL3452

3 Samples

Download data: TXT

(Submitter supplied) We have performed a genome wide investigation for the binding locations of the transcriptional co-repressor proteins Ssn6, Tup11 and Tup12 in the fission yeast Schizosaccharomyces pombe. We have used a ChIP protocol described previously (Robyr et al, 2003) with microarrays containing ORF and IGR fragments representing the complete fission yeast genome (Wiren et al, 2005). Keywords: ChIP-CHIP

Organism:

Schizosaccharomyces pombe

Type:

Genome binding/occupancy profiling by genome tiling array

Platforms:

GPL3452 GPL3453

3 Samples

Download data: TXT

(Submitter supplied) We have performed a genome wide investigation for the binding locations of the transcriptional co-repressor proteins Ssn6, Tup11 and Tup12 in the fission yeast Schizosaccharomyces pombe. We have used a ChIP protocol described previously (Robyr et al, 2003) with microarrays containing ORF and IGR fragments representing the complete fission yeast genome (Wiren et al, 2005). Keywords: ChIP-CHIP

Organism:

Schizosaccharomyces pombe

Type:

Genome binding/occupancy profiling by genome tiling array

Platform:

GPL3452

3 Samples

Download data: TXT

(Submitter supplied) We have performed a genome wide expression profile study of the essential transcriptional co-repressor protein Ssn6 in Schizosaccharomyces pombe. Ssn6 affected targets were identified by over expression of Ssn6 protein in a WT strain. A WT strain holding a plasmid expressing Ssn6 (pDUAL-Ssn6) was compared against the expression in a WT with the empty plasmid (pDUAL-EMPTY) strain. Keywords: Overexpression/WT

Organism:

Schizosaccharomyces pombe

Type:

Expression profiling by array

Platform:

GPL3454

2 Samples

Download data: TXT

(Submitter supplied) We have performed a genome wide expression profile study of the transcriptional co-repressor proteins tup11,12 in Schizosaccharomyces pombe. The expression of a double knock out strain (tup11,12D) was compared against the expression in a WT strain under normal conditions YES-media at 30 degrees (Xue et al, 2004). Keywords: DeletionMutant/WT

Organism:

Schizosaccharomyces pombe

Type:

Expression profiling by array

Platform:

GPL3451

2 Samples

Download data: TXT

(Submitter supplied) Loss of Hst3 and Hst4 leads to increased nascent transcription which increases the prevelance of R-loops and contributes to DNA damage phenotype of the mutant

Organism:

Saccharomyces cerevisiae

Type:

Expression profiling by high throughput sequencing; Other

Platform:

GPL19756

27 Samples

Download data: BEDGRAPH, BW, TXT, XLSX

(Submitter supplied) ChIP-chip analyses of Abp1 and Cbh1 across the S. pombe genome. Keywords: ChIP-chip

Organism:

Schizosaccharomyces pombe

Type:

Genome binding/occupancy profiling by genome tiling array

Platform:

GPL4682

4 Samples

Download data: TXT

(Submitter supplied) Sir2 and the homologous proteins, Hst1, Hst2, Hst3, and Hst4 from Saccharomyces cerevisiae are NAD+-dependent histone deacetylases of the sirtuin protein family. Sir2 functions in transcriptional silencing at the silent mating-type loci, telomeres, and rDNA locus, but also promotes replicative lifespan. To gain a better understanding of the chromatin-regulatory roles carried out by Sir2 and the Hst proteins, we performed ChIP-sequencing analysis on all five sirtuins and Sum1, the DNA binding partner for Hst1. more...

Organism:

Saccharomyces cerevisiae

Type:

Genome binding/occupancy profiling by high throughput sequencing

Platform:

GPL9377

7 Samples

Download data: BEDGRAPH

(Submitter supplied) In eukaryotic cells , histone deactylase plays key roles in the diverse process such as genomic stabillity, differenciation, and metabolism. These enzymes also conserved in filamentous fungi, and their functios has been started to understand some fungal biological process such as development, stress responce, and secondary metabolism. We found deletion of HstD homolog of yeast Hst4, affected development, secondary metabolism in aspergillyus oryzae. more...

Organism:

Aspergillus oryzae RIB40; Aspergillus oryzae

Type:

Expression profiling by array

Platform:

GPL16184

4 Samples

Download data: CEL, CHP

(Submitter supplied) The transcription profile of Candida glabrata grown under two different Niacin limitation conditions were determined. Condition 1 is comparing log phase C. glabrata cells (O.D. 0.5-0.6) grown in synthetic medium containing 0.016 uM versus 3.25 uM nicotinic acid (NA), a common form of Niacin. The NA concentration of 3.25 uM is the standard concentration in synthetic complete (SC) medium. Condition 2 is comparing log phase C. more...

Organism:

Nakaseomyces glabratus

Type:

Expression profiling by array

Platform:

GPL3922

10 Samples

Download data: GPR

(Submitter supplied) The hst3hst4 strain (FY background) has the HST3 and HST4 genes, encoding putative NAD-dependent deacetylases that regulate histone 3 K56 acetylation, deleted. Expression profiling using Affymetrix microarrays was used to assess the change in the gene expression in this strain in comparison to wild-type under normal growth conditions.

Organism:

Saccharomyces cerevisiae

Type:

Expression profiling by array

Platform:

GPL90

4 Samples

Download data: CEL