GEO DataSets

(Submitter supplied) Expression profiling of treatment of E. histolytica HM-1:IMSS trophozoites with 5-Aza cytidine, an inhibitor of DNA methyltransferase Keywords: drug treatment

Organism:

Entamoeba histolytica

Type:

Expression profiling by array

Platform:

GPL4622

4 Samples

Download data: CEL

(Submitter supplied) In order to dtermine the role of histone actylation in gene expresssion, E. histolytica 200:NIH trophozoites were treated with the HDAC inhibitor Trichostatin A, or grown in short chain fatty acids, which have been shown to alter histone actylation patterns in Entamoeba species. Keywords: Expression

Organism:

Entamoeba histolytica

Type:

Expression profiling by array

Platform:

GPL4622

8 Samples

Download data: CEL

(Submitter supplied) Entamoeba histolytica is a protozoan parasite that causes colitis and liver abscesses. Several Entamoeba species and strains with differing levels of virulence have been identified. E. histolytica HM-1:IMSS is a virulent strain, E. histolytica Rahman is a nonvirulent strain, and Entamoeba dispar is a nonvirulent species. We used an E. histolytica DNA microarray consisting of 2,110 genes to assess the transcriptional differences between these species/strains with the goal of identifying genes whose expression correlated with a virulence phenotype. more...

Organism:

Entamoeba histolytica

Type:

Expression profiling by array

Dataset:

GDS1825

Platform:

GPL2662

12 Samples

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Expression profiling of virulent and non-virulent Entamoeba strains and species. Entamoeba histolytica is a protozoan parasite that causes colitis and liver abscesses. Results identify expression patterns indicative of a virulence phenotype.

Organism:

Entamoeba histolytica

Type:

Expression profiling by array, log2 ratio, 2 other, 2 species, 3 strain sets

Platform:

GPL2662

Series:

GSE4139

12 Samples

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(Submitter supplied) Amebiasis is an intestinal disease transmitted by the protist parasite, Entamoeba histolytica, following the ingestion of contaminated food and water. Many infected patients (90%) are asymptomatic but for unknown reasons, these trophozoites can become virulent and invasive, cause amebic dysentery, and migrate to the liver, via the portal veins, where they cause hepatocellular damage. We have recently reported that Escherichia coli can modulate E.histolytica 's virulence and resistance to oxidative stress (OS) via the production of oxaloacetate. more...

Organism:

Entamoeba histolytica

Type:

Expression profiling by high throughput sequencing

Platform:

GPL24064

11 Samples

Download data: XLS

(Submitter supplied) We examined the stress response in Entamoeba histolytica trophozoites by comparing untreated log-phase HM-1:IMSS trophozoites to those subjected to heat shock at 42C for 1 hour. Keywords: stress reponse

Organism:

Entamoeba histolytica

Type:

Expression profiling by array

Platform:

GPL4622

4 Samples

Download data: CEL

(Submitter supplied) Abstract: We have developed an Entamoeba histolytica genomic DNA microarray and used it to develop a transcriptional profile of 1,971 E. histolytica (HM-1:IMSS) genes. The arrays accurately detected message abundance and 31-47% of amebic genes were expressed under standard tissue culture conditions (levels detectable by Northern blot analysis or RT-PCR respectively). Genes expressed at high levels ( approximately 2% of total) included actin (8.m00351), and ribosomal genes (20.m00312). more...

Organism:

Entamoeba histolytica

Type:

Expression profiling by array

Platforms:

GPL2661 GPL2662

25 Samples

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(Submitter supplied) Entamoeba histolytica is a protozoan parasite which causes colitis and liver abscesses. Using a genomic DNA microarray consisting of 1.6 - 2.0 kb genomic inserts we have generated a transcriptional profile of 1,971 unique parasite transcripts. The arrays in this experiment set were used to (1) estimate relative transcript abundance for Entamoeba histolytica (HM-1:IMSS) trophozoites in mid-logarithmic growth and (2) to examine changes in the transcriptional profile of Entamoeba histolytica when the parasite interacts with colonic epithelial cells (Caco-2). more...

Organism:

Entamoeba histolytica

Type:

Expression profiling by array

Platform:

GPL2662

21 Samples

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(Submitter supplied) Entamoeba histolytica is a protozoan parasite which causes colitis and liver abscesses. A pilot microarray consisting of 360 unique parasite genes was constructed using identical methods to the larger array (1,971 unique genes). The four arrays in this data set were used to ascertain whether the microarrays would be useful in detecting changes in transcript abundance by exposing parasites to heat shock (42 0C for 1 hr). more...

Organism:

Entamoeba histolytica

Type:

Expression profiling by array

Platform:

GPL2661

4 Samples

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(Submitter supplied) We compared multiple strains of lab trophozoites to recent clinical isolates. Clinical isolates were grown in xenic media, and maintained many characteristics of the cyst stage of devlopment Keywords: Stage conversion

Organism:

Entamoeba histolytica

Type:

Expression profiling by array

Platform:

GPL4622

19 Samples

Download data: CEL

(Submitter supplied) A genome-wide transcriptional analysis of Entamoeba histolytica was performed on trophozoites isolated from the colon of six infected mice and from in vitro culture. Adaptation to the intestinal environment was accompanied by increases in a subset of cell signaling genes including transmembrane kinases, ras and rho family GTPases, and calcium binding proteins. Significant decreases in mRNA abundance for genes involved in glycolysis and concomitant increases in lipases were consistent with a change in energy metabolism. more...

Organism:

Entamoeba histolytica

Type:

Expression profiling by array

Platform:

GPL4622

7 Samples

Download data: CEL

(Submitter supplied) A custom generated Affymetrix platform full genome microarray (E_his-1a520285F). This array has 7,712 unique probe sets representing 9,435 open reading frames (ORFs). Due to the highly repetitive nature of the E. histolytica genome, some of the probe sets are predicted to cross-hybridize with other sequences. Probe sets that represent a single gene are labeled as (_at). Probe sets in which at least one probe may cross-hybridize with another gene(s) are labeled as (_x_at). more...

Organism:

Entamoeba histolytica

10 Series

67 Samples

Download data: CDF

(Submitter supplied) E. histolytica of strains HM1:IMSS (virulent) and Rahman (avirulent) were examined for response to oxidative and nitrosative stress.

Organism:

Entamoeba histolytica

Type:

Expression profiling by array

Platform:

GPL4622

15 Samples

Download data: CEL

(Submitter supplied) We provided a full spectrum analysis for E. histolytica AGO2-2 associated 27nt small RNAs. Additionally, comparative analysis of small RNA populations from virulent and non-virulent amebic strains indicates that small RNA populations may regulate virulence genes.

Organism:

Entamoeba histolytica

Type:

Non-coding RNA profiling by high throughput sequencing

Platform:

GPL16534

2 Samples

Download data: FA, TXT

(Submitter supplied) To know the role of Rab7 isotypes in enteric protozoan parasite Entamoeba histolytica, one out of nine Rab7 isotypes, Rab7D, was specifically silenced and examined the effect on virulence-related phenotypes. To clarify transcriptomic difference caused by rab7d gene silencing, RNA-seq analysis was conducted.

Organism:

Entamoeba histolytica

Type:

Expression profiling by high throughput sequencing

Platform:

GPL24064

6 Samples

Download data: XLSX

(Submitter supplied) The ability of Entamoeba histolytica to phagocytose host cells correlates to observed virulence in vivo. To better understand the mechanism of phagocytosis we used paramagnetic beads coated with host ligand and sorted trophozoites based on phagocytic ability. Gene expression was then measured in both the sorted phagocytic and non-phagocytic populations using a custom Affymetrix chip for E. histolytica. more...

Organism:

Entamoeba histolytica

Type:

Expression profiling by array

Platform:

GPL9693

6 Samples

Download data: CEL

(Submitter supplied) The unicellular eukaryote Entamoeba histolytica is a human parasite that causes amebic dysentery and liver abscess. A genome-wide analysis of gene expression modulated by intestinal colonization and invasion identified an up-regulated transcript that encoded a putative high-mobility-group box (HMGB) protein, EhHMGB1. We tested if EhHMGB1 encoded a functional HMGB protein, and determined its role in control of parasite gene expression. more...

Organism:

Entamoeba histolytica

Type:

Expression profiling by array

Platform:

GPL4622

6 Samples

Download data: CEL

(Submitter supplied) This SuperSeries is composed of the SubSeries listed below.

Organism:

Entamoeba invadens; Entamoeba histolytica

Type:

Non-coding RNA profiling by high throughput sequencing

Platforms:

GPL19933 GPL19934

7 Samples

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(Submitter supplied) We analyzed ~27nt small RNAs from Entamoeba invadens trophozoites, 24h cysts, 72h cysts, and excysting cells (8h)

Organism:

Entamoeba invadens

Type:

Non-coding RNA profiling by high throughput sequencing

Platform:

GPL19934

4 Samples

Download data: TXT

(Submitter supplied) We analyzed ~27nt small RNAs from Entamoeba histolytica trophozoites in basal conditions and after heat shock or oxidative stress

Organism:

Entamoeba histolytica

Type:

Non-coding RNA profiling by high throughput sequencing

Platform:

GPL19933

3 Samples

Download data: TXT