GEO DataSets

(Submitter supplied) The alkylating agent N-methyl-N’-nitro-N-nitrosoguanidine (MNNG) induces cellular DNA damages and other comprehensive alterations that lead to chromosomal aberrations, mutations, tumor initiations, and cell death. However, the molecular mechanism of MNNG-induced cellular stress remains unclear.We have genome-wide analyzed early transcriptional responses of human FL amnion epithelial cells after exposure to three relatively low doses of MNNG (0.2, 1.0, and 10.0µM),and differential gene expression profiles were obtained 4 h after exposure using oligonucleotide microarrays followed by validation with quantitative real-time RT-PCR. more...

Organism:

Homo sapiens

Type:

Expression profiling by array

Platforms:

GPL96 GPL97

16 Samples

Download data: CEL, EXP

(Submitter supplied) The environmental carcinogen, (±)-anti-benzo[a]pyrene-7,8-diol-9,10-epoxide (BPDE), causes bulky-adduct DNA damages, triggers certain signaling pathways, and elicits gene expression changes. Here, we focused on the temporal gene expression changes induced by a low concentration (0.05 µM) BPDE in human amnion epithelial FL cells. Differential gene expression profiles at 1, 10 and 22 h post BPDE treatment were obtained using Affymetrix HG-U133 Plus 2.0 oligonucleotide microarrays. more...

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL570

18 Samples

Download data: CEL, EXP

(Submitter supplied) d-serine is naturally present throughout the human body. It is also used as add-on therapy for treatment-refractory schizophrenia. d-Serine interacts with the strychnine-insensitive glycine binding site of NMDA receptor, and this interaction could lead to potentially toxic activity (i.e., excitotoxicity) in brain tissue. The transcriptomic changes that occur in the brain after d-serine exposure have not been fully explored. more...

Organism:

Rattus norvegicus

Type:

Expression profiling by array

Dataset:

GDS3643

Platform:

GPL1355

24 Samples

Download data: CEL

Analysis of forebrains of animals treated with up to 500 mg/kg D-serine for 96 hours. D-serine is involved in many physiological processes through its interaction with the glycine binding site of the NMDA receptor. Results provide insight into the impact of D-serine exposure on neuronal functions.

Organism:

Rattus norvegicus

Type:

Expression profiling by array, count, 2 agent, 6 dose sets

Platform:

GPL1355

Series:

GSE10748

24 Samples

Download data: CEL

(Submitter supplied) Prolonged use of glucocorticoids can lead to the formation of a cataract, however the mechanism is not known. We recently reported the presence of the functional glucocorticoid receptor in immortalized cultured mammalian lens epithelial cells (Gupta & Wagner, Invest Ophthalmol Vis Sci 2003), but the short term biological effect of glucocorticoid action in lens epithelial cells is not known. This study seeks to examine glucocorticoid induced changes in global gene expression in LECs. more...

Organism:

Homo sapiens

Type:

Expression profiling by array

Dataset:

GDS1353

Platform:

GPL96

12 Samples

Download data

Analysis of cultured immortalized lens epithelial cells (LEC) 4 and 16 hours after treatment with 1 uM dexamethasone, a glucocorticoid steroid hormone. Long-term glucocorticoid use can induce cataract formation. Results identify glucocorticoid targets and short-term effects of glucocorticoid on LECs

Organism:

Homo sapiens

Type:

Expression profiling by array, count, 2 agent, 2 time sets

Platform:

GPL96

Series:

GSE3040

12 Samples

Download data

(Submitter supplied) MCF-7 and HepG2 cells were exposed to a range of concentrations of benzo(a)pyrene or benzo(e)pyrene (0-5 uM) for up to 48 h and gene expression analysis performed. Keywords: dose response

Organism:

Homo sapiens

Type:

Expression profiling by array

4 related Platforms

96 Samples

Download data

(Submitter supplied) RNA isolated from the cultures treated with 0 and 50 micromolar AFB1 at 15, 30, 60, 90, 120 min was used for microarray experiments. For each array hybridization experiment, RNAs from the treated sample and its corresponding time-matched control were co-hybridized to arrays and respectively quantified in different channels. A dye swap strategy was used to eliminate the dye bias. Keywords: time-course

Organism:

Saccharomyces cerevisiae

Type:

Expression profiling by array

Platform:

GPL1895

11 Samples

Download data

(Submitter supplied) RNA isolated from the 0, 10, 25, 50 and 100 micromolar AFB1 cultures at 120 min treatment was used for cDNA microarray experiments. For each array hybridization experiment, RNAs from the treated sample and its corresponding time-matched control were co-hybridized to arrays and respectively quantified in different channels. A dye swap strategy was used to eliminate the dye bias. Keywords: dose response

Organism:

Saccharomyces cerevisiae

Type:

Expression profiling by array

Platform:

GPL1895

10 Samples

Download data

(Submitter supplied) This study aimed to identify genes that are linked with optineurin expression using a combined siRNA-microarray approach Keywords: siRNA treatment study

Organism:

Homo sapiens

Type:

Expression profiling by array

Dataset:

GDS2892

Platform:

GPL570

6 Samples

Download data: CEL

Analysis of HeLa cells depleted for optineurin using RNAi knockdown. Mutations in the gene for optineurin are associated with open-angle glaucoma. Results provide insight into the function of optineurin and its role in the pathogenesis of open-angle glaucoma.

Organism:

Homo sapiens

Type:

Expression profiling by array, transformed count, 2 protocol sets

Platform:

GPL570

Series:

GSE6819

6 Samples

Download data: CEL

(Submitter supplied) This SuperSeries is composed of the SubSeries listed below.

Organism:

Homo sapiens

Type:

Expression profiling by array

Dataset:

GDS2926

Platform:

GPL887

77 Samples

Download data

(Submitter supplied) Little is known about the global transcriptional program underlying megakaryocytic (Mk) differentiation, maturation, and apoptosis. Using DNA microarrays and Q-RT-PCR, we examined the transcriptional profile of Mk differentiation human CD34-positive hematopoietic stem and progenitor cells cultured with thrombopoietin, interleukin-3, and Flt3-ligand. The goal this study was to identify genes involved in the various facets of Mk differentiation including commitment, polyploidization, proplatelet formation, and apoptosis. more...

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL887

36 Samples

Download data

(Submitter supplied) Little is known about the global transcriptional program underlying megakaryocytic (Mk) differentiation, maturation, and apoptosis. Using DNA microarrays and Q-RT-PCR, we examined the transcriptional profile of phorbol-ester-induced Mk differentiation of the megakaryoblastic CHRF-288-11 (CHRF) cell line – a model system for investigating megakaryopoiesis. The goals of this study were to (1) verify the megakaryocytic nature of the CHRF cell line at the transcriptional level, and (2) extract novel insights into the key facets of Mk maturation including polyploidization, proplatelet formation, and apoptosis. more...

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL887

41 Samples

Download data

Temporal analysis of phorbol ester-treated CHRF-288-11 megakaryoblastic cells induced to undergo megakaryocytic (Mk) differentiation and primary Mk (PriMk) cells derived from cytokine-treated CD34+ peripheral blood cells. Results provide insight into molecular mechanisms underlying megakaryopoiesis.

Organism:

Homo sapiens

Type:

Expression profiling by array, log e ratio, 4 agent, 2 cell line, 13 time sets

Platform:

GPL887

Series:

GSE8914

77 Samples

Download data

(Submitter supplied) B lymphoblast samples from control individual, Scott syndrome patient and Scott syndrome daughter. From each samples one part was treated with A23187 ca2+ ionophore. Treated and non-treated samples were then cultured further. Each sample was then processed in duplicate for hybridization on the microarray Keywords: ordered

Organism:

Homo sapiens

Type:

Expression profiling by array

Dataset:

GDS1320

Platform:

GPL8300

12 Samples

Download data

Analysis of Scott B lymphoblasts after calcium ionophore A23187 treatment. Lymphoblasts derived from a patient with Scott syndrome, a hemorrhagic disease. Results provide insight into the mechanisms underlying the apoptotic effect of A23187, and susceptibility of Scott B lymphoblasts to apoptosis.

Organism:

Homo sapiens

Type:

Expression profiling by array, count, 2 agent, 3 disease state, 3 individual sets

Platform:

GPL8300

Series:

GSE1028

12 Samples

Download data

(Submitter supplied) Using various exposure conditions, we studied the induction of DNA-protein crosslinks (DPX) by formaldehyde (FA) and their removal in primary human nasal epithelial cells (HNEC). DPX were indirectly measured by the alkaline comet assay as the reduction of gamma ray – induced DNA migration. DPX are the most relevant primary DNA alterations induced by FA and the comet assay is a very sensitive method for the detection of FA-induced DPX. more...

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL570

39 Samples

Download data: CEL

(Submitter supplied) Prostaglandin F2 alpha (PGF2alpha) brings about regression of the bovine corpus luteum (CL). This luteolytic property of PGF2alpha is used in the beef and dairy cattle to synchronize estrus. A limitation of this protocol is an insensitivity of the early CL to luteolytic actions of PGF2alpha. The mechanisms underlying luteal sensitivity (LS) are poorly understood. The early CL has maximum number of PGF2alpha receptors; therefore differences in signaling events might be responsible for LS. more...

Organism:

Bos taurus

Type:

Expression profiling by array

Platform:

GPL3810

1 Sample

Download data: GPR

(Submitter supplied) To understand the host transcriptional response to S. enterica serovar Choleraesuis (S. Choleraesuis), the first generation Affymetrix porcine GeneChip® was used to identify differentially expressed genes in the mesenteric lymph nodes responding to infection at acute (8 hours (h), 24h, 48h post-inoculation (pi)) and chronic stages (21 days (d) pi) The objectives of this study were to identify and examine the stereotypical gene expression response within the host mesenteric lymph nodes to S. more...

Organism:

Sus scrofa

Type:

Expression profiling by array

Platform:

GPL3533

15 Samples

Download data: CEL, EXP