GEO DataSets

(Submitter supplied) Methotrexate (MTX) has been widely used for the treatment of a variety of tumors as well as for inflammatory diseases and rheumatoid arthritis (RA). MTX-induced toxicity has been a serious unpredictable side effect of the treatment and an important clinical problem. Possible causes include allergic, cytotoxic or immunologic reactions to this agent. We examined the consequences of the mechanism of MTX-induced pulmonary toxicity gene expression in BEAS-2B cells, huma bronchial cell line, by microarray. more...

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL1708

3 Samples

Download data: GPR

(Submitter supplied) Mitogen-activated protein kinases (MAPKs) regulate cardiomyocyte growth and apoptosis in response to extracellular stimulation, but the downstream effectors that mediate their pathophysiological effects remain poorly understood. We determined the targets and role of p38 MAPK in the heart in vivo by using local adenovirus-mediated gene transfer of constitutively active upstream kinase mitogen-activated protein kinase kinase 3b (MKK3bE) and wild-type p38α in rats. more...

Organism:

Rattus norvegicus

Type:

Expression profiling by array

Dataset:

GDS2487

Platform:

GPL1355

10 Samples

Download data: CEL, EXP

Analysis of left heart ventricles of transgenics overexpressing the mitogen-activated protein kinase (MAPK) p38 in their hearts. p38 is involved in the pathophysiology of inflammatory diseases. Results provide insight into the role of p38 in the heart.

Organism:

Rattus norvegicus

Type:

Expression profiling by array, count, 2 protocol sets

Platform:

GPL1355

Series:

GSE3866

10 Samples

Download data: CEL, EXP

(Submitter supplied) The alkylating agent N-methyl-N’-nitro-N-nitrosoguanidine (MNNG) induces cellular DNA damages and other comprehensive alterations that lead to chromosomal aberrations, mutations, tumor initiations, and cell death. However, the molecular mechanism of MNNG-induced cellular stress remains unclear.We have genome-wide analyzed early transcriptional responses of human FL amnion epithelial cells after exposure to three relatively low doses of MNNG (0.2, 1.0, and 10.0µM),and differential gene expression profiles were obtained 4 h after exposure using oligonucleotide microarrays followed by validation with quantitative real-time RT-PCR. more...

Organism:

Homo sapiens

Type:

Expression profiling by array

Platforms:

GPL96 GPL97

16 Samples

Download data: CEL, EXP

(Submitter supplied) Infection of the human host by Streptococcus pneumoniae begins with colonization of the nasopharynx, which is mediated by adherence of bacteria to respiratory epithelium. Several studies have indicated an important role for the pneumococcal capsule in this process. Here, we used microarrays to characterize the in vitro transcriptional response of human nasopharyngeal epithelial Detroit 562 cells to adherence of serotype 2-encapsulated strain D39, serotype 19F-encapsulated strain G54, serotype 4-encapsulated strain TIGR4, and their nonencapsulated derivatives (Δcps). more...

Organism:

Homo sapiens

Type:

Expression profiling by array

Dataset:

GDS3041

Platform:

GPL570

32 Samples

Download data: CEL, EXP

Analysis of pharyngeal epithelial cells exposed to various encapsulated strains of Streptococcus pneumoniae. Nasopharynx colonization by S. pneumoniae is mediated by adherence to the respiratory epithelium. Results provide insight into the capsule-dependent host response to pneumococcal adherence.

Organism:

Homo sapiens

Type:

Expression profiling by array, count, 5 agent, 3 cell type, 3 dose, 2 infection sets

Platform:

GPL570

Series:

GSE8527

32 Samples

Download data: CEL, EXP

(Submitter supplied) A genome-scale assessment of peripheral blood B-cell molecular homeostasis in patients with rheumatoid arthritis. Keywords: Rheumatoid Arthritis, B-cells

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL3463

2 Samples

Download data

(Submitter supplied) AAmiodarone is an antiarrhythmic drug and representatively induce pulmonary phospholipidosis. Amiodarone-induced toxicity has been a serious unpredictable side effect of the treatment and an important clinical problem. Possible causes include allergic, cytotoxic or immunologic reactions to this agent. We examined the consequences of the mechanism of amiodarone-induced pulmonary toxicity gene expression in BEAS-2B cells, huma bronchial cell line, by microarray. more...

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL1708

3 Samples

Download data: TXT

(Submitter supplied) Total RNA was isolated from candoxin-treated (n = 3 flasks of each time points - 12hrs, 24hrs and 48hrs) and untreated control (n = 3) cells using RNeasy® mini kit.The preparation and processing of labeled, fragmented cRNA for oligonucleotide mcroarray hybridization has been performed according to the manufacturer’s protocols described in the technical manual (Affymetrix, Santa Clara, CA). Keywords: time-course

Organism:

Homo sapiens

Type:

Expression profiling by array

Dataset:

GDS1414

Platform:

GPL96

12 Samples

Download data

Analysis of glial cell line Hs683 at various time points up to 48 hours following exposure to 300 nM of candoxin (CDX) snake venom. CDX is a neurotoxin that inhibits post-synaptic neuromuscular and neuronal alpha7nACh-receptors, and delays cell death in glial cells.

Organism:

Homo sapiens

Type:

Expression profiling by array, count, 2 agent, 4 time sets

Platform:

GPL96

Series:

GSE1682

12 Samples

Download data

(Submitter supplied) Non-steroidal anti-inflammatory drugs (NSAIDs) are used extensively as therapeutic agents, despite their well-documented gastrointestinal (GI) toxicity. Presently, the mechanisms responsible for NSAID-associated GI damage are incompletely understood. In this study, we used Microarray analysis to generate a novel hypothesis about cellular mechanisms that underlie the GI toxicity of NSAIDs. Monolayers of intestinal epithelial cells (IEC-6) were treated with NSAIDs that either exhibit indomethacin, NS-398) or lack (SC-560) inhibitory effects on intestinal epithelial cell migration. more...

Organism:

Rattus norvegicus

Type:

Expression profiling by array

Platform:

GPL1355

14 Samples

Download data: CEL, CHP

(Submitter supplied) Comparison of transcript abundance between control (untreated) and methotrexate treated S3 Drosophila cells and ovaries disected from female flies. Keywords: Stress response

Organism:

Drosophila melanogaster

Type:

Expression profiling by array

Platforms:

GPL1467 GPL1473

6 Samples

Download data

(Submitter supplied) Clopidogrel is associated with a series of gastrointestinal side effects, such as bleeding complications and recurrent gastric ulcer; however, their mechanisms are largely unclear. In this study, human gene expression microarray and gene ontology analysis were utilized to evaluate the effects of clopidogrel on gene expression in human gastric epithelial cells (GES-1) Keywords: Clopidogrel; Gastric injury; GES-1 cell line

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL6480

6 Samples

Download data: TXT

(Submitter supplied) This study proposes a molecular mechanism for lung epithelial A549 cell response to copper oxide nanoparticles (CuO-NPs) related to Cu ions released from CuO-NPs. Cells that survived exposure to CuO-NPs arrested the cell cycle as a result of the downregulation of proliferating cell nuclear antigen (PCNA), cell division control 2 (CDC2), cyclin B1 (CCNB1), target protein for Xklp2 (TPX2), and aurora kinase A (AURKA) and B (AURKB). more...

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL6480

1 Sample

Download data: GPR

(Submitter supplied) Fragmentary knowledge exists on the adverse health effects of CoO- and CeO2-nanoparticles (NP)s. We analyzed toxicogenomic profiles of BEAS-2B versus A549 cells using genome-wide transcriptomics to identify molecules and cellular processes that are triggered by monodispersed noncytotoxic suspensions of 7-nm CoO- and 4-nm CeO2-NPs for 3, 6, 10, and 24 hours. We aimed to investigate 1) whether a cell type responds similarly to two different NPs exposure, 2) whether alveolar versus bronchial epithelial cells respond differently to the same NP, and 3) whether immune processes are influenced. more...

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL4133

48 Samples

Download data: TXT

(Submitter supplied) The purpose of this work was to identify the potential signatures of cobalt exposure using a toxicogenomic approach. A time course transcriptome analysis was performed on human type II epithelial cell line A549. Cells were exposed to cobalt at midlog phase ; a medium without FCS containing cobalt (CoCl2, Sigma) or not was added for 30mn, 2h, 4h or 24h. Total RNA was isolated using the Quiagen RNeasy miniprep kit, and then labelled using the FairPlay Microarray Labelling Kit (Stratagene). more...

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL4263

30 Samples

Download data

(Submitter supplied) BACKGROUND: Cadmium is implicated in prostate carcinogenesis, but its oncogenic action remains unclear. OBJECTIVES: In this study we aimed to decipher changes in cell growth and the transcriptome in an immortalized human normal prostate epithelial cell line (NPrEC) following exposure to low-dose Cd. METHODS: Synchronized NPrEC cells were exposed to different doses of Cd and assayed for cell viability and cell-cycle progression. more...

Organism:

Homo sapiens

Type:

Expression profiling by array

Dataset:

GDS3354

Platform:

GPL570

19 Samples

Download data: CEL

Analysis of immortalized normal prostate epithelial cell line NPrEC exposed to non-cytotoxic levels of cadmium (2.5uM CdCl2) for up to 32hrs. Cadmium is implicated in prostate carcinogenesis. Results provide insight into mechanisms underlying the initiation of carcinogenesis by Cd in the prostate.

Organism:

Homo sapiens

Type:

Expression profiling by array, transformed count, 2 agent, 5 time sets

Platform:

GPL570

Series:

GSE9951

19 Samples

Download data: CEL

(Submitter supplied) As part of the civil aviation safety program to define the adverse effects of ethanol on flying performance, we present results of our DNA microarray analysis of samples from a timecourse study of individuals given ethanol orally, and then evaluated by breathalyzer to monitor blood alcohol content (BAC). At five blood alcohol levels, T1-T5, blood was drawn such that the samples represented 0%, 0.04%, 0.08% BAC, and return to 0.04%, and 0.02% BAC. more...

Organism:

Homo sapiens

Type:

Expression profiling by array

Datasets:

GDS4938 GDS6177

Platform:

GPL570

54 Samples

Download data: CEL

Analysis of blood from subjects administered orange juice w/o alcohol. Blood collected at time points corresponding to collection times for the alcohol group in GDS4938. These results, together with those from GDS4938, provide insight into molecular response of blood during acute ethanol exposure.

Organism:

Homo sapiens

Type:

Expression profiling by array, transformed count, 5 individual, 2 protocol, 5 time sets

Platform:

GPL570

Series:

GSE20489

25 Samples

Download data: CEL