GEO DataSets

(Submitter supplied) The euryhaline marine yeast Debaromyces hansenii is a model system for the study of genes related to osmotic stress. To study the transcriptional response of this organism to osmotic stress we have used the two color microarray based gene expression analysis of 6211 genes which constitutes the whole genome. Analysis was done at three time points after induction with salt (0.5h, 3h and 6h.) The mRNA level of 64 genes significantly increased at least 3-fold after induction with 2M NaCl whereas that of 45 genes was 3-fold diminished. more...

Organism:

Debaryomyces hansenii

Type:

Expression profiling by array

Platform:

GPL6287

6 Samples

Download data: TXT

(Submitter supplied) Schizosaccharomyces pombe 4976 ORFs spotted in duplicate on glass slide Keywords = yeast ORF DNA microarray Reference: Xue Y, Haas SA, Brino L, Gusnanto A et al. A DNA microarray for fission yeast: minimal changes in global gene expression after temperature shift. Yeast 2004 Jan 15;21(1):25-39. PMID: 14745780 Protocol: Primary PCR: The PCR reactions are performed using tagged genome specific primers in the presence of genomic DNA (provided by your laboratory). more...

Organism:

Schizosaccharomyces pombe

3 Series

3 Samples

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(Submitter supplied) Schizosaccharomyces pombe 4976 ORFs spotted in duplicate on glass slide Keywords = yeast ORF DNA microarray Protocol: Primary PCR: The PCR reactions are performed using tagged genome specific primers in the presence of genomic DNA (provided by your laboratory). We normally achieve a success rate of 93 % of PCR reactions. For the failed PCR reactions we perform three different protocols of PCR conditions, which include different MgCl2 concentrations and different Taq polymerase. more...

Organism:

Schizosaccharomyces pombe

4 Series

14 Samples

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(Submitter supplied) Schizosaccharomyces pombe 4976 ORFs spotted in duplicate on glass slide Keywords = yeast ORF DNA microarray

Organism:

Schizosaccharomyces pombe

1 Series

2 Samples

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(Submitter supplied) Drought, salinity and sub-optimal temperatures are stresses that cause adverse effects on the growth of plants and the productivity of crops. In this study, we have analyzed the expression profiles of rice genes under control and abiotic stress conditions using microarray technology to identify the genes differentially expressed during various abiotic stresses. Keywords: Stress treatment

Organism:

Oryza sativa

Type:

Expression profiling by array

Platform:

GPL2025

12 Samples

Download data: CEL

(Submitter supplied) Plants initially undergo a period of vegetative development, in which it produces leaves from shoot apical meristem (SAM) and roots from the root apical meristem. Later in development, the SAM undergoes a change in fate and enters reproductive development called as floral transition, producing flowers and seeds. Our understanding of the molecular and genetic mechanisms that underlie reproductive development in plants has increased tremendously in the past decade, essentially through the work on Arabidopsis. more...

Organism:

Oryza sativa

Type:

Expression profiling by array

Platform:

GPL2025

45 Samples

Download data: CEL

(Submitter supplied) Indica rice seedlings of IR64 variety were grown hydroponically for 7-days in a culture room with a daily photoperiodic cycle of 14h light and 10h dark. Seedlings were incubated in 0.1% dimethyl sulfoxide (control) or 50 micromolar solutions of indole-3-acetic acid (IAA treatment) and benzyl aminopurine (BAP treatment) for 1h and 3h. Equal amounts of 1h and 3h samoles were pooled for each treatment before RNA isolation. more...

Organism:

Oryza sativa

Type:

Expression profiling by array

Platform:

GPL2025

6 Samples

Download data: CAB, CEL, EXP

(Submitter supplied) Using a C. elegans whole genome DNA microarray in this study, the effects of five different xenobiotics on the gene expression of the nematode were investigated. The exposure time for the following five applied compounds beta-NF (5 mg/l), Fla (0.5 mg/l), atrazine (25 mg/l), clofibrate (10 mg/l) and DES (0.5 mg/l) was 48+/-5 h. The analysis of the data showed a clear induction of 203 genes belonging to different families like the cytochromes P450, UDP-glucoronosyltransferases (UDPGT), glutathione S-transferases (GST), carboxylesterases, collagenes, C-type lectins and others. more...

Organism:

Caenorhabditis elegans

Type:

Expression profiling by array

5 related Platforms

16 Samples

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(Submitter supplied) Microarray technology provides a powerful tool for gene discovery studies, but the development of microarrays for individual species can be expensive and time-consuming. In this study, we test the suitability of a Danio rerio oligonucleotide microarray for application in a species with few genomic resources, the coral reef fish Pomacentrus moluccensis. Coral reef fishes are expected to experience rising sea surface temperatures due to climate change. more...

Organism:

Danio rerio; Pomacentrus moluccensis

Type:

Expression profiling by array; Genome variation profiling by array

Platform:

GPL3365

8 Samples

Download data: TIFF

(Submitter supplied) Microarray analysis was used to identify the osmotic stress-responsive genes dependent on HogA and AtfA in the filamentous fungus Aspergillus nidulans. In order to identify such genes, we conducted the several types of experiment. One was a comparison between wild type with and without osmotic shock (Exp.6). Others were comparison between wild type with osmotic shock and each mutant (hogA, Exp.7; atfA, Exp.8) with osmotic shock. more...

Organism:

Aspergillus nidulans

Type:

Expression profiling by array

Platform:

GPL7364

6 Samples

Download data: GPR

(Submitter supplied) Microarray analysis was used to identify the fludioxonil-responsive genes dependent on SskA, SrrA, HogA, and AtfA in the filamentous fungus Aspergillus nidulans. In order to identify such genes, we conducted the several types of experiment. One was a comparison between wild type treated with fludioxonil and without the treatment (Exp.1). Others were comparison between wild type treated with fludioxonil and each mutant (sskA, Exp.2; srrA, Exp.3; hogA, Exp.4; atfA, Exp.5) treated with fludioxonil. more...

Organism:

Aspergillus nidulans

Type:

Expression profiling by array

Platform:

GPL7364

10 Samples

Download data: GPR

(Submitter supplied) In this study, using DNA microarray analysis, we compared the comprehensive expression profiles of two yeast trains, i.e, the previously obtained ethanol-adapted yeast strain and the parental strain as control (FY834), under the ethanol stress condition (YPD medium contained 10% ethanol). As a result, we identified certain genes and functional categories of the genes that are possible involved in growth under the ethanol stress condition.

Organism:

Saccharomyces cerevisiae

Type:

Expression profiling by array

Platform:

GPL7698

2 Samples

Download data: GPR

(Submitter supplied) We explored transcriptional responses of the chytridiomycete Blastocladiella emersonii to some stress environmental conditions. Three distinct cDNA libraries constructed from mRNAs extracted from cells submitted to heat shock and different cadmium concentrations (50 and 100 mM) were sequenced and generated 6,350 high-quality EST sequences. These ESTs were assembled into 2,326 unigenes, being 51% of them not previously described in B. more...

Organism:

Blastocladiella emersonii

Type:

Expression profiling by array

Platform:

GPL4516

42 Samples

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(Submitter supplied) Primary productivity of open ocean environments, such as those inhabited by marine picocyanobacteria Synechococcus sp.WH8102, are often limited by low inorganic phosphate (P). To observe how this organism copes with P starvation, we constructed a full genome microarray and examined differences in gene expression under P-limited and P-replete growth conditions. To determine the temporal nature of the responses, comparisons were made for cells newly entered into P-stress (at a time point corresponding to the induction of extracellular alkaline phosphatase activity) and a later time point (late log phase). more...

Organism:

Parasynechococcus marenigrum WH 8102

Type:

Expression profiling by array

Platforms:

GPL7449 GPL7448

23 Samples

Download data: TAV

(Submitter supplied) Although major differences in the response of tissues were documented in the literature, the molecular mechanisms underlying tissue radiosensitivity are still fragmentary and need to be study more thoroughly. To this end, a genome wide analysis strategy was used to investigate changes in gene expression in the liver following exposition to ionizing radiation. This follows the tissue survey that was previously initiated with other organs and tends to obtain a comprehensive picture of the molecular events underlying tissue radio sensitivity. more...

Organism:

Rattus norvegicus; Mus musculus

Type:

Expression profiling by array

Platforms:

GPL4376 GPL4377

16 Samples

Download data: GPR

(Submitter supplied) Transcriptional profiling of bacteroids isolated from the soybean root nodules. Keywords: life style comparison

Organism:

Bradyrhizobium diazoefficiens USDA 110; Bradyrhizobium japonicum

Type:

Expression profiling by array

Platform:

GPL5341

6 Samples

Download data: GPR

(Submitter supplied) Functional genomics of B. japonicum exposed to osmotic stress caused by 50 mM NaCl. Keywords: stress response

Organism:

Bradyrhizobium japonicum; Bradyrhizobium diazoefficiens USDA 110

Type:

Expression profiling by array

Platform:

GPL5341

4 Samples

Download data: GPR

(Submitter supplied) Functional genomics of B. japonicum in minimal vs. rich medium. Different nutritional status was compared. Keywords: stress response

Organism:

Bradyrhizobium diazoefficiens USDA 110; Bradyrhizobium japonicum

Type:

Expression profiling by array

Platform:

GPL5341

6 Samples

Download data: TXT

(Submitter supplied) Taking advantage of the availability of the Tuber melanosporum genome sequence and a whole-genome microarray, we wanted to test the hypothesis that the transition from a growth temperature of 24-25°C to a cold one (4°C) is perceived by the filamentous fungus at the transcriptomic level and we wanted to identify and characterize the genes involved in this stress response.

Organism:

Tuber melanosporum

Type:

Expression profiling by array

Platform:

GPL8982

6 Samples

Download data: PAIR, TXT

(Submitter supplied) Bacteria respond to osmotic stress by a substantial increase in the intracellular osmolality, adjusting their cell turgor for altered growth conditions. Using E. coli as a model organism we demonstrate here that bacterial responses to hyperosmotic stress specifically depend on the nature of osmoticum used. We show that increasing acute hyperosmotic NaCl stress above ~1.0 Os kg-1 causes a dose-dependent K+ leak from the cell, resulting in a substantial decrease in cytosolic K+ content and a concurrent accumulation of Na+ in the cell. more...

Organism:

Escherichia coli

Type:

Expression profiling by array

Dataset:

GDS3421

Platform:

GPL3154

6 Samples

Download data: CEL, CHP, TXT