GEO DataSets

(Submitter supplied) Global gene expression profiling of the avian B-lymphoma DT40 cell line was used as a model to differentiate among Btk KO and Btk KO cells reconstituted with human Btk. Differences in the gene expression pattern showed statistically significant changes between parental DT40 and all the Btk KO cell populations irrespective of whether they are reconstituted or not. These results imply that in the process of generating a knockout cell line, subclones are selected, which have multiple changes in their gene expression pattern (p<0.01). more...

Organism:

Gallus gallus

Type:

Expression profiling by array

Platform:

GPL3213

28 Samples

Download data: CEL, CHP, DCP

(Submitter supplied) Bruton's tyrosine kinase (Btk) is important for B lymphocyte development. To identify genes that are differentially expressed in primary B cells lacking functional Btk, splenocytes from X-linked immunodeficiency (Xid), Btk knockout (KO) and immunocompetent CBA mice, were used in microarrays containing more than 12,000 genes and expressed sequence tags (ESTs). We found 4515 transcripts expressed in duplicate experiments in all three strains. more...

Organism:

Mus musculus

Type:

Expression profiling by array

Dataset:

GDS1346

Platform:

GPL81

6 Samples

Download data: CEL, EXP

Analysis of splenic B cells from mutants with a knockout of Bruton's tyrosine kinase (Btk) or a R28C missense mutation in Btk. Btk mutations cause a block in B cell differentiation and lead to X-linked agammaglobulinemia (XLA). Results provide insight into the pathogenesis of XLA.

Organism:

Mus musculus

Type:

Expression profiling by array, count, 3 genotype/variation sets

Platform:

GPL81

Series:

GSE2826

6 Samples

Download data: CEL, EXP

(Submitter supplied) Splenic Transitional Type-1 B-cells from CBA wild-type mice, X-linked immunodeficiency mice and Bruton's tyrosine kinase knock-out mice. Two replicates where run on Affymetrix 420 2.0 arrays for CBA wild-type, Xid samples and the Btk KO samples. Bruton's tyrosine kinase (Btk) is a cytoplasmic tyrosine kinase important for B-lymphocyte maturation. Mutations in Btk give rise to the primary immunodeficiency disease X-linked agammaglobulinemia (XLA) in man and X-linked immunodeficiency (Xid) in mice. more...

Organism:

Mus musculus

Type:

Expression profiling by array

Platform:

GPL1261

6 Samples

Download data: CEL