GEO DataSets

(Submitter supplied) Transcriptional profiling for global characterization of gene expression alterations that resulted from treatment of melanoma cells with siRNA specifically targeting NRASQ61R

Organism:

Homo sapiens

Type:

Expression profiling by array

Dataset:

GDS3517

Platform:

GPL96

51 Samples

Download data: CEL

Analysis of melanoma 224 and BL cells for up to 3 days after suppression of the mutant NRAS Q61R gene with siRNA siMut10 or siMut12. Activating mutations in NRAS gene is a common genetic event in malignant melanoma. Results provide insight into the role of NRAS in the pathogenesis of melanoma.

Organism:

Homo sapiens

Type:

Expression profiling by array, count, 4 agent, 2 cell line, 4 time sets

Platform:

GPL96

Series:

GSE12445

51 Samples

Download data: CEL

(Submitter supplied) Expression .CEL files from Affymetrix HG-U133A 2.0 arrays using DNA from 14 human cell lines derived from metastasized melanoma

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL571

14 Samples

Download data: CEL

(Submitter supplied) We compared the transformation efficiencies of mutant NRAS and KRAS in immortal, non-transformed Ink4a/Arf-deficient melanocytes. NRAS mutation leads to increased cellular proliferation and is potently tumorigenic. In contrast, KRAS mutation does not enhance melanocyte proliferation and is only weakly tumorigenic on its own. While both NRAS and KRAS activate MAPK signaling, only NRAS enhances MYC activity in these cells. more...

Organism:

Mus musculus

Type:

Expression profiling by array

Platform:

GPL4487

12 Samples

Download data: GPR

(Submitter supplied) Mutations in the NRAS oncogene are present in up to 20% of melanoma. Here, we show that interferon alpha-inducible protein 6 (IFI6) is necessary for NRASQ61K-induced transformation and melanoma growth. IFI6 was transcriptionally upregulated by NRASQ61K, and knockdown of IFI6 resulted in DNA replication stress due to dysregulated DNA replication via E2F2. This stress consequentially inhibited cellular transformation and melanoma growth via senescence or apoptosis induction depending on the RB and p53 pathway status of the cells. more...

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL10558

6 Samples

Download data: TXT

(Submitter supplied) Mutations in the NRAS oncogene are present in up to 20% of melanoma. Here, we show that interferon alpha-inducible protein 6 (IFI6) is necessary for NRASQ61K-induced transformation and melanoma growth. IFI6 was transcriptionally upregulated by NRASQ61K, and knockdown of IFI6 resulted in DNA replication stress due to dysregulated DNA replication via E2F2. This stress consequentially inhibited cellular transformation and melanoma growth via senescence or apoptosis induction depending on the RB and p53 pathway status of the cells. more...

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL10558

12 Samples

Download data: TXT