GEO DataSets

(Submitter supplied) Recent studies have highlighted the role of adrenal corticosteroid signaling in cardiac physiology and pathophysiology. It is known that glucocorticoids and aldosterone are able to bind glucocorticoid receptor (GR) and mineralocorticoid receptor (MR), and these ligand-receptor interactions are redundant. Therefore, it has been impossible to delineate how these nuclear receptors couple with corticosteroid ligands and differentially regulate gene expression for operation of their distinct functions in the heart. more...

Organism:

Rattus norvegicus

Type:

Expression profiling by array

Platform:

GPL1355

12 Samples

Download data: CEL

(Submitter supplied) Gene expression analysis was conducted using Agilent Human1Av2 arrays (Agilent Technologies, Palo Alto, CA). Two separate biological replicates of cytoplasmic RNA samples were harvested and purified from each of the U-2 OS cell lines stably expressing hGRalpha, -A, -B, -C3, or D3 treated with 100 nM DEX or vehicle for 6 hours using RNeasy Midi kits (Invitrogen). These RNA samples were amplified using the Agilent Low RNA Input Fluorescent Linear Amplification Kit protocol. more...

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL887

20 Samples

Download data: TIFF, TXT

(Submitter supplied) The human glucocorticoid receptor (GR) is expressed as two alternately spliced Cterminal isoforms: α and β. In contrast to the canonical hGRα, hGRβ is constitutively nuclear, is not thought to bind ligand, and is believed to affect gene transcription only by acting as a dominant negative to hGRα. Microarray analysis indicated that hGRβ, expressed in the absence of hGRα, can regulate gene expression, and furthermore, occupation of hGRβ with the antagonist RU-486 diminishes that capacity. more...

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL887

24 Samples

Download data: TIFF, TXT

(Submitter supplied) d-serine is naturally present throughout the human body. It is also used as add-on therapy for treatment-refractory schizophrenia. d-Serine interacts with the strychnine-insensitive glycine binding site of NMDA receptor, and this interaction could lead to potentially toxic activity (i.e., excitotoxicity) in brain tissue. The transcriptomic changes that occur in the brain after d-serine exposure have not been fully explored. more...

Organism:

Rattus norvegicus

Type:

Expression profiling by array

Dataset:

GDS3643

Platform:

GPL1355

24 Samples

Download data: CEL

Analysis of forebrains of animals treated with up to 500 mg/kg D-serine for 96 hours. D-serine is involved in many physiological processes through its interaction with the glycine binding site of the NMDA receptor. Results provide insight into the impact of D-serine exposure on neuronal functions.

Organism:

Rattus norvegicus

Type:

Expression profiling by array, count, 2 agent, 6 dose sets

Platform:

GPL1355

Series:

GSE10748

24 Samples

Download data: CEL

(Submitter supplied) Stress induced elevation in plasma cortisol levels is shown to be involved in the metabolic re-adjustments to stress but the genetic basis of this response is still unclear. Cortisol signaling in teleosts is thought to be mediated predominantly by the glucocorticoid receptor (GR), a ligand-bound transcription factor and previous studies have shown that RU486, a GR antagonist offsets corticosteroid signaling in teleosts. more...

Organism:

Oncorhynchus mykiss

Type:

Expression profiling by array

Platform:

GPL3713

17 Samples

Download data

(Submitter supplied) Background and Aims: It is known that inflammatory processes are activated in heart failure, but the regulation of cytokines and their role in the pathogenesis of the disease are not well understood. To address this issue, we have performed microarray analysis of non-infarcted left ventricular tissue from mice at various time-points after myocardial infarction. Methods: Molecular alterations in myocardial tissue were measured 3, 5, 7 and 14 days after induced infarction by using cDNA microarrays. more...

Organism:

Mus musculus

Type:

Expression profiling by array

Dataset:

GDS3386

Platform:

GPL891

20 Samples

Download data

Analysis of non-infarcted left ventricular tissues from animals at various time points up to 14 days following myocardial infarction (MI). MI induced by ligating the left coronary artery. Results provide insight into the molecular pathogenesis of heart failure.

Organism:

Mus musculus

Type:

Expression profiling by array, log2 ratio, 4 time sets

Platform:

GPL891

Series:

GSE6580

20 Samples

Download data

(Submitter supplied) The objective of this study was to determine how dexamethasone and KLF15 modify GR and RNAPII occupancy in human airway smooth muscle on a genome-wide basis.

Organism:

Homo sapiens

Type:

Genome binding/occupancy profiling by high throughput sequencing

Platform:

GPL11154

20 Samples

Download data: NARROWPEAK

(Submitter supplied) We previously demonstrated that the transcription factor, KLF15, is a glucocorticoid-regulated gene that represses primary human airway smooth muscle (ASM) proliferation. Here, we show that KLF15 also represses ASM hypertrophy. To uncover the mechanistic basis for these effects, we integrated transcriptome data from KLF15 over-expression with genome-wide analysis of RNA Polymerase II (RNAPII) and glucocorticoid receptor (GR) occupancy (i.e. more...

Organism:

Homo sapiens

Type:

Expression profiling by high throughput sequencing

Platform:

GPL17303

8 Samples

Download data: TXT

(Submitter supplied) The objective of this study was to dertemine GR binding patterns within wild type murine embryonic fibroblasts in comparison to embryonic fibroblasts derived from mice containing a null mutation for Klf15.

Organism:

Mus musculus

Type:

Genome binding/occupancy profiling by high throughput sequencing

Platform:

GPL13112

7 Samples

Download data: BED

(Submitter supplied) In this work, we used our recently developed method Spec-seq to characterize the binding specificity of Glucocorticoid receptor in vitro.

Organism:

Homo sapiens

Type:

Other

Platform:

GPL15520

2 Samples

Download data: TXT

(Submitter supplied) Lung alveolarization is a complex process that involves interactions between several cell types and leads to considerable increase in gas-exchange surface area. The step designated secondary septation includes elastogenesis from interstitial fibroblasts. We used microarrays to detail the global programme of fibroblast gene expression that underly secondary septation, and identified numerous genes that undergo signficant changes in expression Keywords: Time course

Organism:

Rattus norvegicus

Type:

Expression profiling by array

Platform:

GPL1355

9 Samples

Download data: CEL

(Submitter supplied) Aerobic capacity is a strong predictor of cardiovascular mortality. To determine the relationship between aerobic capacity and cardiac gene expression we examined genome-wide gene expression in hearts of rats artificially selected for high- and low running capacity (HCR and LCR, respectively) over 16 generations. HCR were born with an athletic phenotype, whereas LCR exhibited features of the metabolic syndrome. more...

Organism:

Rattus norvegicus

Type:

Expression profiling by array

Dataset:

GDS3131

Platform:

GPL1355

16 Samples

Download data: CEL

Analysis of hearts from low (LCRs) and high capacity runners (HCRs) with or without exercise training. Aerobic capacity is a strong predictor of cardiovascular mortality. Results provide insight into the molecular basis for the difference in inborn aerobic capacity in the LCRs and HCRs.

Organism:

Rattus norvegicus

Type:

Expression profiling by array, transformed count, 2 protocol, 2 strain sets

Platform:

GPL1355

Series:

GSE9445

16 Samples

Download data: CEL

(Submitter supplied) Glucocorticoids, which activate glucocorticoid receptor signaling and thus modulate gene expression, are widely used to treat asthma. Glucocorticoids exert their therapeutic effects in part through modulating airway smooth muscle structure and function. However, the effects of genes that are regulated by GCs on airway function are not fully understood. Here, we used transcription profiling to characterize the effects of a potent glucocorticoid, dexamethasone, on cultured human airway smooth muscle gene expression at 4 and 24 hours.

Organism:

Homo sapiens

Type:

Expression profiling by array

Dataset:

GDS3946

Platform:

GPL6480

10 Samples

Download data: TXT

Analysis of airway smooth muscle (ASM) cells treated with glucocorticoid (GC) dexamethasone for 4 or 24h. GCs are used to treat asthma, exerting their therapeutic effects in part through modulating ASM structure/function. Results provide insight into molecular mechanisms underlying GC action in ASM.

Organism:

Homo sapiens

Type:

Expression profiling by array, transformed count, 2 agent, 3 time sets

Platform:

GPL6480

Series:

GSE34313

10 Samples

Download data: TXT

(Submitter supplied) The glucocorticoid receptor (GR) regulates adaptive transcriptional programs that alter metabolism in response to stress. Network properties that allow GR to tune gene expression to match specific physiologic demands are poorly understood. We analyzed the transcriptional consequences of GR activation in murine lungs deficient for Klf15, a transcriptional regulator of amino acid metabolism that is induced by glucocorticoids and fasting

Organism:

Mus musculus

Type:

Expression profiling by array

Platform:

GPL7202

29 Samples

Download data: TXT

(Submitter supplied) Objective: Thyroid hormone receptors (TRs) are ligand-dependent transcription factors with a major impact on erythroid cell development. Here we investigated TR activity on red cell gene expression and identified TR target genes. The impact of the TR target gene GAR22 (growth arrest specific 2 [GAS2]-related gene on chromosome 22) on red cell differentiation was determined. Methods: SCF/Epo dependent red cell progenitors were differentiated in vitro in the presence or absence of thyroid hormone. more...

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL8300

13 Samples

Download data: CEL, CHP, EXP

(Submitter supplied) Recent clinical data suggest that the efficacy of statin treatment in patients with heart failure varies depending on the drugs administered. Therefore, the present study was undertaken to compare murine cardiac gene expression following treatment with four different statins. Statins directly regulated cardiac gene expression in a drug-specific manner in mice. The influence of each statin on the cardiac mRNA expression of various genes, including several genes that might be involved in the development of cardiac disease, was not a general effect of this class of drugs.

Organism:

Mus musculus

Type:

Expression profiling by array

Platform:

GPL1261

20 Samples

Download data: CEL, CHP