GEO DataSets

(Submitter supplied) Approximatively 75% of the genome of S. aureus (“core” genome) are highly conserved between strains, whereas the remaining 25% (“accessory” genome) are composed of variable regions that are mostly composed of mobile genetic elements (MGE), containing virulence and resistance genes. We have developed a composite DNA-microarray (StaphVar Array) which selectively targets 403 genes located on the accessory or core variable genome. more...

Organism:

Staphylococcus aureus

Type:

Genome variation profiling by array

Platform:

GPL7684

26 Samples

Download data: GPR

(Submitter supplied) Previous studies have documented the diversity of genetic background of methicillin-resistant S. aureus (MRSA) strains associated with healthcare (HA-MRSA), community (CA-MRSA) and livestock (LA-MRSA). The accessory and core-variable genome content of those strains remain largely unknown. To compare the composition of accessory and core-variable genome of Belgian MRSA strains according to host, population setting and genetic background, representative strains of HA- (n=21), CA- (n = 13) and ST398 LA-MRSA (n = 18) were characterized by a DNA-microarray (StaphVar Array) composed of oligonucleotide probes targeting ~400 resistance, adhesion and virulence associated genes.ST398 strains displayed very homogenous hybridization profiles (>94% gene content homology) irrespective of their host origin. more...

Organism:

Staphylococcus aureus

Type:

Genome variation profiling by array

Platform:

GPL7684

104 Samples

Download data: GPR

(Submitter supplied) Streptococcus uberis is one of the principal causative agents of bovine mastitis. The organism is typically considered an environmental pathogen, however, recent studies also suggest possible host adaptation. In this study, two multilocus sequence typing (MLST) schemes and whole genome DNA microarrays were used to evaluate the degree and nature of genome flexibility between S. uberis strains. The 21 isolates examined in this study arise from a collection of 232 international isolates for which previous epidemiological and preliminary genotyping data existed. more...

Organism:

Streptococcus equi subsp. zooepidemicus; Streptococcus uberis; Streptococcus equi subsp. equi

Type:

Genome variation profiling by array

Platforms:

GPL7311 GPL7308

44 Samples

Download data: GPR

(Submitter supplied) Little is known about the expression of methicillin-resistant Staphylococcus aureus (MRSA) genes during infection conditions. Here, we described the transcriptome of the clinical MRSA strain USA300 derived from human cutaneous abscesses, and compared it with USA300 bacteria derived from infected kidneys in a mouse model. Remarkable similarity between the transcriptomes allowed us to identify genes encoding multiple proteases and toxins, and iron- and peptide-transporter molecules, which are upregulated in both infections and are likely important for establishment of infection. more...

Organism:

Staphylococcus aureus subsp. aureus USA300_FPR3757; Staphylococcus aureus subsp. aureus USA300

Type:

Expression profiling by array

Platform:

GPL20994

16 Samples

Download data: TXT

(Submitter supplied) Neutrophil lysis after phagocytosis is a process potentially important in the pathogenesis of community-associated methicillin-resistant S. aureus (CA-MRSA) infection. The mechanism for this process is not currently known. Therefore, to better understand CA-MRSA virulence we used human oligonucleotide microarrays to investigate the mechanism underlying enhanced PMN lysis that occurs after phagocytosis of CA-MRSA. more...

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL570

113 Samples

Download data: CEL, CHP

(Submitter supplied) CodY is a conserved broad acting transcriptional factor that regulates the expression of genes related to amino acid metabolism and virulence in Staphylococcus aureus. CodY target genes have been studied by using in vitro DNA affinity purification and deep sequencing (IDAP-Seq). In this study we performed the first in vivo determination of CodY target genes using a novel CodY monoclonal antibody in established ChIP-exo protocols. more...

Organism:

Staphylococcus aureus

Type:

Expression profiling by high throughput sequencing

Platform:

GPL25144

4 Samples

Download data: CSV

(Submitter supplied) Staphylococcus aureus is one of the most important pathogens in humans and animals, multiply resistant strains are increasingly widespread, new agents are needed for the treatment of S. aureus. Rhein, a natural plant product, has potential antimicrobial activity against Staphylococcus aureus. We employed Affymetrix Staphylococcus aureus GeneChipsTM arrays to investigate the global transcriptional profiling of Staphylococcus aureus ATCC25923 treated with rhein. more...

Organism:

Staphylococcus aureus

Type:

Expression profiling by array

Dataset:

GDS2812

Platform:

GPL1339

6 Samples

Download data: CEL

Analysis of Staphylococcus aureus ATCC25923 cultures treated with subinhibitory concentrations of rhein for 45 minutes. Rhein is an antimicrobial agent from rhubarb. Results provide insight into the efficacy of rhein against Staphylococcus aureus.

Organism:

Staphylococcus aureus

Type:

Expression profiling by array, count, 2 agent sets

Platform:

GPL1339

Series:

GSE8135

6 Samples

Download data: CEL

(Submitter supplied) in vitro comparison between two MRSA grown in rich (BHI) and poor media (SNM), compared with the nasal metatranscriptome reads of S. aureus.

Organism:

Staphylococcus aureus

Type:

Expression profiling by high throughput sequencing

Platforms:

GPL18481 GPL16057

7 Samples

Download data: TXT

(Submitter supplied) Emerging known and unknown pathogens create profound threats to public health. Platforms for rapid detection and characterization of microbial agents are critically needed to prevent and respond to disease outbreaks. Available detection technologies cannot provide broad functional information about known and novel organisms. As a step toward developing such a system, we have produced and tested a series of high-density functional gene arrays to detect elements of virulence and antibiotic resistance mechanisms. more...

Organism:

Staphylococcus aureus subsp. aureus Mu50; Escherichia coli CFT073; Streptococcus pyogenes MGAS5005; Staphylococcus aureus; Escherichia coli O157:H7 str. EDL933; Enterococcus faecalis V583; Staphylococcus saprophyticus; Escherichia coli str. K-12 substr. MG1655; Salmonella enterica; unidentified

Type:

Other

Platform:

GPL6656

12 Samples

Download data: PAIR