GEO DataSets

(Submitter supplied) This SuperSeries is composed of the SubSeries listed below.

Organism:

Danio rerio; Pimephales promelas

Type:

Expression profiling by array

Platforms:

GPL7282 GPL6563

24 Samples

Download data: TXT

(Submitter supplied) This experiment was conducted as part of an overall study aimed at examining effects of a dopamine receptor antagonist on reproduction, behavior, and gene expression in cyprinid fish. Relative to gene expression, we hypothesized that if haloperidol disrupted normal regulation of the HPG axis, there may be robust transcriptional alterations in the ovary of exposed females that might serve as useful markers of exposure and/or effects. more...

Organism:

Pimephales promelas

Type:

Expression profiling by array

Platform:

GPL7282

12 Samples

Download data: TXT

(Submitter supplied) This experiment was conducted as part of an overall study aimed at examining effects of a dopamine receptor antagonist on reproduction, behavior, and gene expression in cyprinid fish. Relative to gene expression, we hypothesized that if haloperidol disrupted normal regulation of the HPG axis, there may be robust transcriptional alterations in the ovary of exposed females that might serve as useful markers of exposure and/or effects. more...

Organism:

Danio rerio

Type:

Expression profiling by array

Platform:

GPL6563

12 Samples

Download data: TXT

(Submitter supplied) Prochloraz is a fungicide known to cause endocrine disruption through effects on the hypothalamic-pituitary-gonadal (HPG) axis. To determine the short-term impacts of prochloraz on gene expression and steroid production, adult female fathead minnows (Pimephales promelas) were exposed to the chemical (0 or 300 μg/L) for a time-course of 6, 12 and 24 h. Consistent with inhibition of cytochrome P450 17α-hydroxylase/17,20-lyase (CYP17) and aromatase (CYP19), known molecular targets of prochloraz, plasma 17β-estradiol (E2) was reduced within 6 hours. more...

Organism:

Pimephales promelas

Type:

Expression profiling by array

Platform:

GPL9248

23 Samples

Download data: TXT

(Submitter supplied) We evaluated the possible mechanisms by which exposures to pulp and paper mill effluents gene expression in the fathead minnow hypothalamus Keywords: Toxicology

Organism:

Pimephales promelas

Type:

Expression profiling by array

Platform:

GPL7351

15 Samples

Download data: TXT

(Submitter supplied) Interpretation of toxicogenomic experiments conducted with ovary tissue from asynchronous-spawning small fish species are complicated by background variation in the relative abundance and proportion of follicles at different stages within the ovary tissue sample. This study employed both real-time quantitative PCR and a 15,000 gene oligonucleotide microarray to examine variation in the fathead minnow (Pimephales promelas) ovarian transcriptional profile as a function of quantitative and qualitative differences in ovarian histology. more...

Organism:

Pimephales promelas

Type:

Expression profiling by array

Platform:

GPL9248

30 Samples

Download data: TXT

(Submitter supplied) Despite major successes in reducing the risks of lead (Pb) exposure over the past few decades, two issues of considerable importance remain unresolved: (1) how differences in water chemistry influence acute and chronic Pb toxicity, and (2) the elucidation of specific toxic mechanisms and modes of action (MOA). To more clearly define the water chemistry parameters mediating Pb toxicity we evaluated the effects of hardness (as CaSO4) and DOC (as humic acid (HA)) during chronic (150d) exposures to the fathead minnow (Pimephales promelas). more...

Organism:

Pimephales promelas

Type:

Expression profiling by array

Platform:

GPL5443

30 Samples

Download data: GPR

(Submitter supplied) Paper Abstract. Extrapolating toxicological sensitivity information across species can require the use of highly conservative safety factors as prescribed by ecological risk assessment protocols. The objective of our study was to use multipule factors to understand the dynamics of stress responses by comparing ecotoxicological, developmental, and genomic data to quantitatively determine the relative sensitivity of two common model aquatic vertebrates to assess the potential for developing refined safety factors among species. more...

Organism:

Danio rerio; Pimephales promelas

Type:

Expression profiling by array

Platforms:

GPL7301 GPL10259

59 Samples

Download data: TXT

(Submitter supplied) Metformin, along with its biotransformation product guanylurea, are commonly observed in municipal wastewaters and subsequent surface waters. Previous studies in fish have identified metformin as a potential endocrine active compound but there are inconsistencies in the literature with regard to effects. To further investigate the potential reproductive toxicity of metformin and guanylurea to fish, a series of experiments were performed with reproductively mature fathead minnows (Pimephales promelas). more...

Organism:

Pimephales promelas

Type:

Expression profiling by high throughput sequencing

Platform:

GPL25572

191 Samples

Download data: TXT

(Submitter supplied) This SuperSeries is composed of the SubSeries listed below.

Organism:

Pimephales promelas; Danio rerio

Type:

Expression profiling by array

Platforms:

GPL6457 GPL9248

66 Samples

Download data: TXT

(Submitter supplied) Effects of bisphenol A (BPA) on ovarian transcript profiles as well as targeted endpoints with endocrine/reproductive relevance were examined in two fish species, fathead minnow (Pimephales promelas) and zebrafish (Danio rerio), exposed in parallel using matched experimental designs. Four days of waterborne exposure to 10 µg BPA caused significant vitellogenin induction in both species. However, zebrafish were less sensitive to effects on hepatic gene expression and steroid production than fathead minnow and the magnitude of vitellogenin induction was more modest. more...

Organism:

Pimephales promelas

Type:

Expression profiling by array

Platform:

GPL9248

32 Samples

Download data: TXT

(Submitter supplied) Effects of bisphenol A (BPA) on ovarian transcript profiles as well as targeted endpoints with endocrine/reproductive relevance were examined in two fish species, fathead minnow (Pimephales promelas) and zebrafish (Danio rerio), exposed in parallel using matched experimental designs. Four days of waterborne exposure to 10 µg BPA caused significant vitellogenin induction in both species. However, zebrafish were less sensitive to effects on hepatic gene expression and steroid production than fathead minnow and the magnitude of vitellogenin induction was more modest. more...

Organism:

Danio rerio

Type:

Expression profiling by array

Platform:

GPL6457

34 Samples

Download data: TXT

(Submitter supplied) This SuperSeries is composed of the SubSeries listed below.

Organism:

Pimephales promelas

Type:

Expression profiling by array; Third-party reanalysis

Platform:

GPL10259

511 Samples

Download data: TXT

(Submitter supplied) Fathead minnow and zebrafish are among the most intensively studied fish species in environmental toxicogenomics. To aid the assessment and interpretation of subtle transcriptomic effects from treatment conditions of interest, there needs to be a better characterization and understanding of the natural variation in gene expression among fish individuals within populations. Little effort, however, has been made in this area. more...

Organism:

Danio rerio

Type:

Third-party reanalysis; Expression profiling by array

Download data: TXT

(Submitter supplied) Fathead minnow and zebrafish are among the most intensively studied fish species in environmental toxicogenomics. To aid the assessment and interpretation of subtle transcriptomic effects from treatment conditions of interest, there needs to be a better characterization and understanding of the natural variation in gene expression among fish individuals within populations. Little effort, however, has been made in this area. more...

Organism:

Pimephales promelas

Type:

Expression profiling by array

Platform:

GPL10259

511 Samples

Download data: TXT

(Submitter supplied) This study aimed to evaluate the utility of microarrays as a biomonitoring tool in field studies. A 15,000-oligonucleotide microarray was used to measure the hepatic gene expression of fathead minnows (Pimephales promelas) caged in four Nebraska, USA watersheds - the Niobrara and Dismal Rivers (low-impact agricultural sites) and the Platte and Elkhorn Rivers (high-impact agricultural sites). Gene expression profiles were site specific and fish from the low- and high-impact sites aggregated into distinct groups. more...

Organism:

Pimephales promelas

Type:

Expression profiling by array

Platform:

GPL7351

16 Samples

Download data: TXT

(Submitter supplied) Gene expression profiles of fathead minnow brain tissue following treatment to fluoxetine (Prozac), carbamazepine, venlafaxine, and a mixture of all three; compared to untreated controls.

Organism:

Pimephales promelas

Type:

Expression profiling by array

Platform:

GPL7351

16 Samples

Download data: TXT

(Submitter supplied) Cyclooxygenase (COX) inhibition is of concern in fish because COX inhibitors (e.g., ibuprofen) are ubiquitous in aquatic systems/fish tissues, and can disrupt synthesis of prostaglandins that modulate a variety of essential biological functions including reproduction. Transcriptomic data and publicly available high throughput toxicity data were utilized to develop putative adverse outcome pathways (AOPs) for molecular initiating event (MIE) of COX inhibition. more...

Organism:

Pimephales promelas

Type:

Expression profiling by array

Platform:

GPL9248

31 Samples

Download data: TXT

(Submitter supplied) Background: Complex biological networks control fundamental processes such as reproduction. The relationship of network structure to biological function was investigated in a global gene interaction network developed from ovary tissues of the model fish, fathead minnow (Pimephales promelas). Results: A global ovary gene interaction network was inferred from gene expression in ovaries of fathead minnow representing 288 different exposure conditions and 1,472 microarrays. more...

Organism:

Pimephales promelas

Type:

Expression profiling by array

Platform:

GPL10259

1470 Samples

Download data: TXT

(Submitter supplied) Modeled on human connectivity mapping (Cmap), this study was undertaken to investigate the potential applications of Cmap approach in ecotoxicology. Over 3500 zebrafish (Danio rerio) and fathead minnow (Pimephales promelas) transcriptomic profiles, each associated with one of several dozen chemical treatment conditions, were compiled into three distinct collections of rank-ordered gene lists (ROGLs) by species and microarray platforms. more...

Organism:

Pimephales promelas

Type:

Expression profiling by array

Platforms:

GPL9248 GPL15775

580 Samples

Download data: TXT