GEO DataSets

(Submitter supplied) In order to examine the mechanism of TPO on cardiac protection against DOX damage, we have employed whole genome microarray expression profiling as a discovery platform to identify genes with the potential to delineate the TPO cardioprotective mechanism against DOX-induced cardiomyopathy

Organism:

Rattus norvegicus

Type:

Expression profiling by array

Platform:

GPL7294

8 Samples

Download data: TXT

(Submitter supplied) We have employed whole genome microarray expression profiling to identify genes differentially expressed in cord blood enriched CD34+ cells(>95%) after a short-term exposure to the chemokine stromal cell-derived factor-1 (SDF-1).

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL570

8 Samples

Download data: CEL, CHP

(Submitter supplied) Homing and engraftment of hematopoietic stem cells (HSCs) to the bone marrow (BM) involve a complex interplay between chemokines, cytokines, and non-peptide molecules. Extracellular nucleotides and their cognate P2 receptors are emerging as key-factors of inflammation and related chemotactic responses. In this study, we investigated the activity of extracellular adenosine-triphosphate (ATP) and uridine-triphosphate (UTP) on CXCL12-stimulated CD34+ HSC chemotaxis. more...

Organism:

Homo sapiens

Type:

Expression profiling by array

Dataset:

GDS2378

Platform:

GPL96

4 Samples

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Analysis of CD34+ hematopoietic stem cells (HSCs) treated with UTP, alpha-chemokine CXCL12, or both. CXCL12 is a HSC chemoattractant secreted by the bone marrow. Results provide insight into the molecular mechanisms underlying the enhancement of CXCL12-dependent HSC migration by UTP.

Organism:

Homo sapiens

Type:

Expression profiling by array, count, 4 agent sets

Platform:

GPL96

Series:

GSE5168

4 Samples

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(Submitter supplied) In order to examine the mechanism of TPO on cardiac protection against myocardial infarction damage (MI), we have employed whole genome microarray expression profiling as a discovery platform to identify genes with the potential to delineate the TPO cardioprotective mechanism against infarction.

Organism:

Rattus norvegicus

Type:

Expression profiling by array

Platform:

GPL7294

6 Samples

Download data: TXT

(Submitter supplied) We have employed whole genome microarray expression profiling to identify genes differentially expressed in human cord blood CD34+ cells overexpressing Regulator of G protein Signaling (RGS) proteins.

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL16686

16 Samples

Download data: CEL

(Submitter supplied) Lin-, CD38-, CD34+ hematopoietic stem cells. Keywords: other

Organism:

Homo sapiens

Type:

Expression profiling by array

Platforms:

GPL96 GPL97

6 Samples

Download data: CEL, EXP

(Submitter supplied) Global gene expressions of human cord blood-derived 18Lineage-negative (18Lin-)CD34+CD38-CD133+GPI-80+ cells (CD34+ HSCs), 18Lin-CD34-CD133+GPI-80+ cells (CD34- HSCs) and 18Lin-CD34+CD133- cells (non-HSCs) were analyzed. Results provide an insight into the molecular mechanisms underlying the self-renewal, maintenance and differentiation of human cord blood-derived CD34+/- HSCs.

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL20844

9 Samples

Download data: TXT

(Submitter supplied) Multiple myeloma (MM) is a clonal plasma cell disorder frequently accompanied by hematopoietic impairment. Genomic profiling of distinct HSPC subsets revealed a consistent deregulation of signaling cascades, including TGF beta signaling, p38MAPK signaling and pathways involved in cytoskeletal organization, migration, adhesion and cell cycle regulation in MM patients.

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL571

43 Samples

Download data: CEL, CHP

(Submitter supplied) The global gene expression profiles of human umbilical cord blood and adult bone marrow CD34+CD33-CD38-Rho(lo)c-kit+ cells, enriched for hematopoietic stem/progenitor cells (HSC) with CD34+CD33-CD38-Rho(hi) cells, enriched in committed hematopoietic progenitor cells (HPC), were compared to identify candidate regulators of HSC self-renewal versus differentiation fate decisions. Keywords: parallel sample

Organism:

Homo sapiens

Type:

Expression profiling by array

Datasets:

GDS1230 GDS1231

Platforms:

GPL96 GPL97

36 Samples

Download data: CEL, EXP

Comparison of CD34+CD33-CD38-Rho(lo)c-kit+ cells, enriched for hematopoietic stem/progenitor cells (HSC), to CD34+CD33-CD38-Rho(hi) cells, enriched for committed hematopoietic progenitor cells (HPC). Cells obtained from umbilical cord blood and bone marrow. Provides insight into control of HSC fate.

Organism:

Homo sapiens

Type:

Expression profiling by array, count, 2 cell type, 2 tissue sets

Platform:

GPL97

Series:

GSE2666

18 Samples

Download data: CEL, EXP

Comparison of CD34+CD33-CD38-Rho(lo)c-kit+ cells, enriched for hematopoietic stem/progenitor cells (HSC), to CD34+CD33-CD38-Rho(hi) cells, enriched for committed hematopoietic progenitor cells (HPC). Cells obtained from umbilical cord blood and bone marrow. Provides insight into control of HSC fate.

Organism:

Homo sapiens

Type:

Expression profiling by array, count, 2 cell type, 2 tissue sets

Platform:

GPL96

Series:

GSE2666

18 Samples

Download data: CEL, EXP

(Submitter supplied) In the present study, we investigated whether, and to what extent, P2Rs and their ligands are involved in the regulation of AML cells. Our findings show that AML blasts express several receptors belonging to the P2X and P2Y family. Although different samples respond differently to ATP and UTP stimulation (reflecting the variability intrinsic to the group of acute myeloid leukemias), all the tested samples appear to be responsive to purinergic signalling, as demonstrated by intracellular calcium mobilization. more...

Organism:

Homo sapiens

Type:

Expression profiling by array

Dataset:

GDS4304

Platform:

GPL570

24 Samples

Download data: CEL, CHP

Analysis of lymphoblasts from 12 acute myeloid leukemia (AML) patients cultured for 24hrs with ATP. Extracellular nucleotide ATP inhibits proliferation and accumulation of AML cells. Results provide insight into the molecular mechanisms underlying the inhibitory activity of ATP on leukemic cells.

Organism:

Homo sapiens

Type:

Expression profiling by array, count, 2 agent, 4 disease state sets

Platform:

GPL570

Series:

GSE30903

24 Samples

Download data: CEL, CHP

(Submitter supplied) To date, different experimental strategies have been developed for the ex vivo expansion of human hematopoietic stem (HSCs) in clinical application. However, it is still unclear to what difference in genomic function in HSCs expansion under different culture systems. In this study, we compared gene-expression profile of ex vivo expanded serum (10% FBS, fetal bovine serum) and serum-free culture systems, and then analyze molecular function of differentially expressed gene using microarray chips.

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL16686

4 Samples

Download data: CEL

(Submitter supplied) Nucleotides triphosphates are extracellular messengers binding to specific plasma membrane receptors (P2Rs) that modulate responses as different as proliferation, differentiation, migration or cell death on several cell types including hematopoietic stem cells. Little and controversial information is available on the role of extracellular nucleotides in human mesenchimal stem cells (hMSCs). In this study, we assessed whether P2Rs are expressed and functional in bone marrow-derived hMSCs. more...

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL570

2 Samples

Download data: CEL, CHP

(Submitter supplied) Robust human-goat chimerism was achieved by transplanting human CD34+Lin- cord blood cells into fetal goats. We observed a broad distribution of GFP-marked human cells in non-hematopoietic organs including kidney, muscle, lung, and heart of transplant goats. Various marker techniques indicated that human genes were expressed in chimeric livers and blood. Keywords: other

Organism:

Homo sapiens; Capra hircus

Type:

Expression profiling by array

Dataset:

GDS1023

Platform:

GPL96

23 Samples

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Human-goat chimerism achieved by transplanting human CD34+Lin- cord blood cells into fetal goats. Markers indicate human genes expressed in goat liver and blood. Human/goat hematopoietic stem cell (HSC) xenogeneic model allows analysis of HSC transplantation, expansion and differentiation in vivo.

Organism:

Homo sapiens; Capra hircus

Type:

Expression profiling by array, count, 2 protocol, 2 species, 2 tissue sets

Platform:

GPL96

Series:

GSE1801

23 Samples

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(Submitter supplied) The low homing and engraftment efficiency especially in context of low availability of donor CD34+ HSPCs is a major challenge, which limits the clinical applications of HSC therapies. SDF-1/CXCR4 axis plays a principle role in the homing and engraftment of hematopoietic stem/progenitor cells. Activation of CXCR4 induces cell trafficking and homing to the marrow microenvironment, where it retains hematopoietic stem cells in close contact with marrow stromal cells. more...

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL13252

12 Samples

Download data: TXT

(Submitter supplied) Gene expression of adherent cells in a coculture of the human bone marrow stromal cell line HS-27a and peripheral blood monocytes (CD14+ cells) was compared to HS-27a cultured alone. Microarray experiments were conducted using CD14+ cells from 7 healthy donors over a period of >7 months. Monocytes were isolated by ficoll separation of whole blood, followed by labelling cells with CD14 monoclonal antibody Tuek4 and magnetic bead selection (Milteny rat anti mouse IgG2a+2b beads) on an AutoMacs. more...

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL44

7 Samples

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