GEO DataSets

(Submitter supplied) The face is one of the three regions most frequently affected by congenital defects in humans. In order to understand the molecular mechanisms involved it is necessary to have a more complete picture of gene expression in the embryo. Here we use microarrays to profile expression in chicken facial prominences, post neural crest migration and prior to differentiation of mesenchymal cells. Chip-wide analysis revealed that maxillary and mandibular prominences had similar expression profiles while the frontonasal mass chips were distinct. more...

Organism:

Gallus gallus

Type:

Expression profiling by array

Platform:

GPL3213

9 Samples

Download data: CEL, CHP

(Submitter supplied) The patterning of the facial midline involves early specification of neural crest cells to form skeletal tissues that support the upper jaw . In order to understand the molecular mechanisms involved we have taken advantage of a beak duplication model developed in the chicken embryo. Here we can induce the transformation of the side of the beak into a second midline that is easily identifiable by the formation of a supernumerary egg tooth. more...

Organism:

Gallus gallus

Type:

Expression profiling by array

Platform:

GPL3213

12 Samples

Download data: CEL, CHP

(Submitter supplied) This SuperSeries is composed of the SubSeries listed below.

Organism:

Leishmania major; Leishmania mexicana

Type:

Expression profiling by array

Platform:

GPL3505

11 Samples

Download data: TXT

(Submitter supplied) We examined the Leishmania mexicana transcriptome to identify differentially regulated mRNAs using high-density whole-genome oligonucleotide microarrays designed from the genome data of a closely related species, Leishmania major. Statistical analysis on array hybridization data representing 8156 predicted coding regions revealed 288 genes (3.5% of all genes) whose steady-state mRNA levels meet criteria for differential regulation between promastigotes and lesion-derived amastigotes. more...

Organism:

Leishmania major; Leishmania mexicana

Type:

Expression profiling by array

Platform:

GPL3505

9 Samples

Download data: TXT

(Submitter supplied) We examined the Leishmania mexicana transcriptome to identify differentially regulated mRNAs using high-density whole-genome oligonucleotide microarrays designed from the genome data of a closely related species, Leishmania major. This experiment appears as Fig. 1 of the associated publication. Keywords: RNA expression profiling

Organism:

Leishmania mexicana; Leishmania major

Type:

Expression profiling by array

Platform:

GPL3505

4 Samples

Download data: TXT

(Submitter supplied) This platform supports 11 60-mer DNA oligonucleotide probes synthesized twice on each slide for 8160 (98%) of the Leishmania major Friedlin genes annotated at www.genedb.org. Refer to: Timothy R. Holzer, W.R. McMaster, James D. Forney. “Expression profiling by whole-genome interspecies microarray hybridization reveals differential gene expression in procyclic promastigotes, lesion-derived amastigotes, and axenic amastigotes in Leishmania mexicana.” Molecular and Biochemical Parasitology 146 (2): 198-218. more...

Organism:

Leishmania major

3 Series

11 Samples

Download data

(Submitter supplied) The anterior pituitary is comprised of five major hormone-secreting cell types which differentiate during embryonic development in a temporally distinct manner. Microarrays containing 5,128 unique cDNAs expressed in the neuroendocrine system were produced and used to identify genes with potential involvement in onset of thyroid-stimulating hormone beta-subunit, growth hormone, and prolactin gene transcription during chick embryonic development. more...

Organism:

Gallus gallus

Type:

Expression profiling by array

Dataset:

GDS1952

Platform:

GPL1744

16 Samples

Download data: TIFF

(Submitter supplied) A major research focus in our laboratory is the development of the chicken pituitary gland. As this tissue does not yield sufficient RNA to analyze using cDNA microarrays, mRNA must be amplified by reverse transcription with an oligo-dT primer containing the T7 RNA polymerase promoter site followed by in vitro transcription using T7 RNA polymerase. Therefore, this preliminary study investigating differential gene expression between embryonic day 17, when all the major cell types in the chick anterior pituitary gland have differentiated, and post-hatch day 3 was conducted to verify that amplified antisense RNA could be used in conjunction with our neuroendocrine microarrays containing 5,128 unique cDNAs to detect changes in gene expression. more...

Organism:

Gallus gallus

Type:

Expression profiling by array

Dataset:

GDS1951

Platform:

GPL1744

6 Samples

Download data: TIFF

(Submitter supplied) Spotted amplified cDNA array on glass. Produced with cDNA clones expressed in the chicken neuroendocrine system. Tissues used to produced the cDNA library included the hypothalamus, anterior pituitary gland, and pineal gland. 7200 spots Keywords = spotted Keywords = cDNA

Organism:

Gallus gallus

2 DataSets

2 Series

22 Samples

Download data

Analysis of embryonic pituitary glands at various ages from day 10 to day 17 using a neuroendocrine system specific cDNA microarray. Results identify genes potentially involved in the onset of thyroid-stimulating hormone beta-subunit, growth hormone, and prolactin gene transcription.

Organism:

Gallus gallus

Type:

Expression profiling by array, log2 ratio, 4 age sets

Platform:

GPL1744

Series:

GSE3227

16 Samples

Download data: TIFF

Analysis of pituitary glands from day 17 embryos and post-hatch day 3 animals using a neuroendocrine system specific cDNA microarray. Major cell types in the anterior pituitary gland have differentiated at embryonic day 17. Results used to validate the method of amplifying RNA from pituitary glands.

Organism:

Gallus gallus

Type:

Expression profiling by array, log2 ratio, 2 age sets

Platform:

GPL1744

Series:

GSE3226

6 Samples

Download data: TIFF

(Submitter supplied) The aim of this study was to assess the impact of oocyte competence on subsequent fertility. Based on knowledge already accessible in mammals and on bioinformatics tools including the chicken genome sequence, we focused on the expression of genes involved in the processes of fertilization and of early embryo development. The study was performed using two complementary approaches: a descriptive study of standard laying hens and then a differential study performed with hens from experimental lines expressing broad variations of achieved fertility (approximately 20 per cent). more...

Organism:

Gallus gallus

Type:

Expression profiling by array

Platform:

GPL3213

8 Samples

Download data: CEL

(Submitter supplied) Tumor tissue heterogeneity is a well known feature of several solid tumors. Neuroblastic Tumors (NTs) is a group of paediatric cancers with a great tissue heterogeneity. Most of NTs are composed of undifferentiated, poorly differentiated or differentiating neuroblastic (Nb) cells with very few or absent Schwannian stromal (SS) cells: these tumors are grouped as Neuroblastoma (Schwannian stroma-poor). more...

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL96

28 Samples

Download data: CEL

(Submitter supplied) The amino acid homocysteine increases in the serum when there is insufficient folic acid or vitamin B12, or with certain mutations in enzymes important in methionine metabolism. Elevated homocysteine is related to increased risk for cardiovascular and other diseases in adults and elevated maternal homocysteine increases the risk for certain congenital defects, especially those that result from abnormal development of the neural crest and neural tube. more...

Organism:

Gallus gallus

Type:

Expression profiling by array

Dataset:

GDS2646

Platform:

GPL3213

6 Samples

Download data: CEL

Analysis of cultured cardiac neural crest cells treated with homocysteine (HC). Elevated maternal HC increases the risk for congenital defects that result from abnormal development of the neural crest and neural tube. Results provide insight into the molecular basis of HC-induced dysmorphogenesis.

Organism:

Gallus gallus

Type:

Expression profiling by array, count, 2 agent sets

Platform:

GPL3213

Series:

GSE6868

6 Samples

Download data: CEL

(Submitter supplied) Activity-dependent changes in synapses rely on functional changes in resident proteins and on gene expression. We addressed the relationship between synapse activity and the expression of synaptic genes by comparing RNA levels in the neocortex of normal mice versus synaptically silent munc18-1 null mutants, using microarray expression analysis, quantitative PCR and Northern blotting. We hypothesized that genes under control of synaptic activity are differentially expressed between mutants and controls and found that few synaptic signaling genes were differentially expressed. more...

Organism:

Mus musculus

Type:

Expression profiling by array

Platform:

GPL3106

11 Samples

Download data

(Submitter supplied) Activity-dependent changes in synapses rely on functional changes in resident proteins and on gene expression. We addressed the relationship between synapse activity and the expression of synaptic genes by comparing RNA levels in the neocortex of normal mice versus synaptically silent munc18-1 null mutants, using microarray expression analysis, quantitative PCR and Northern blotting. We hypothesized that genes under control of synaptic activity are differentially expressed between mutants and controls and found that few synaptic signaling genes were differentially expressed. more...

Organism:

Mus musculus

Type:

Expression profiling by array

Platform:

GPL3106

4 Samples

Download data

(Submitter supplied) Activity-dependent changes in synapses rely on functional changes in resident proteins and on gene expression. We addressed the relationship between synapse activity and the expression of synaptic genes by comparing RNA levels in the neocortex of normal mice versus synaptically silent munc18-1 null mutants, using microarray expression analysis, quantitative PCR and Northern blotting. We hypothesized that genes under control of synaptic activity are differentially expressed between mutants and controls and found that few synaptic signaling genes were differentially expressed. more...

Organism:

Mus musculus

Type:

Expression profiling by array

Platform:

GPL3106

6 Samples

Download data

(Submitter supplied) Meat type chickens have limited capacities to cope with high environmental temperatures, sometimes leading to important mortality in farms and subsequent economic loss. One of the strategies to increase the sustainability of broiler chicken production is to enhance their adaptive capacities to face heat exposure using heat conditioning (C) during embryogenesis. This strategy was shown to improve thermotolerance in later life. more...

Organism:

Gallus gallus

Type:

Expression profiling by array

Platform:

GPL20679

64 Samples

Download data: TXT

(Submitter supplied) The aim of this study was to assess the impact of oocyte competence on subsequent fertility. Based on knowledge already accessible in mammals and on bioinformatics tools including the chicken genome sequence, we focused on the expression of genes involved in the processes of fertilization and of early embryo development. A differential kinetic study is performed on INRA lines selected on the basis of their fertility potential in purpose of hopefully access gene markers of fertility performance. more...

Organism:

Gallus gallus

Type:

Expression profiling by array

Platform:

GPL3213

6 Samples

Download data: CEL