GEO DataSets

(Submitter supplied) An important, but rarely performed, test of Koch’s molecular postulates involves evaluating the capacity of candidate virulence genes to confer pathogenicity in otherwise non-virulent species. Unbiased genomic surveys of avirulent natural isolates might reveal rare variants possessing specific virulence features, which might prove useful in testing their functional sufficiency. Using a custom pan-genome array, we analyzed a panel of avirulent Burkholderia thailandensis (Bt) isolates related to Burkholderia pseudomallei (Bp), the causative agent of the often fatal human and animal disease melioidosis. more...

Organism:

Burkholderia thailandensis; Burkholderiales

Type:

Genome variation profiling by genome tiling array

Platform:

GPL9490

49 Samples

Download data: PAIR

(Submitter supplied) Introduction. Burkholderia thailandensis is a clinically rare opportunistic pathogen in the genus Burkholderia, and the genomic features and virulence characteristics of B. thailandensis strains that cause human infection remain unclear.Gap Statement. B. thailandensis strains with different virulence induce different host innate immune responses in vitro.Aim. This work aimed to understand the sequence diversity, phylogenetic relationship, and virulence of B. more...

Organism:

Burkholderia thailandensis

Type:

Expression profiling by high throughput sequencing

Platform:

GPL23641

6 Samples

Download data: TXT

(Submitter supplied) Burkholderia cepacia complex bacteria comprise opportunistic pathogens causing chronic respiratory infections in cystic fibrosis (CF) patients. These microorganisms produce exopolysaccharides which are considered virulence determinants. Here, we report the isolation of an evolved nonmucoid variant (17616nmv) after the ancestor, Burkholderia multivorans ATCC 17616, was subjected to prolonged stationary phase. more...

Organism:

Burkholderia multivorans; Burkholderia cenocepacia J2315; Burkholderia multivorans ATCC 17616

Type:

Expression profiling by array

Platform:

GPL13356

6 Samples

Download data: CEL

(Submitter supplied) Many microbial pathogens express specific virulence traits at distinct growth phases. To investigate the molecular pathways linking bacterial growth to pathogenicity, we characterized the genome-wide growth transcriptome of the tropical pathogen Burkholderia pseudomallei (Bp), the causative agent of melioidosis. Using fine-scale sampling, approximately 17% of all Bp genes were found to display regulated expression during growth, manifested primarily as discrete waves of gene expression tightly associated with distinct growth phases and transition points. more...

Organism:

Burkholderia pseudomallei K96243

Type:

Expression profiling by array

Dataset:

GDS2365

Platform:

GPL4078

47 Samples

Download data: GPR

Analysis of Burkholderia pseudomallei at the early, log, and stationary phases of growth. B. pseudomallei causes melioiosis. Results provide insight into the molecular pathways that link growth phase to pathogenicity.

Organism:

Burkholderia pseudomallei K96243

Type:

Expression profiling by array, log2 ratio, 4 other, 47 time sets

Platform:

GPL4078

Series:

GSE5495

47 Samples

Download data: GPR

(Submitter supplied) We report the application of single-molecule-based sequencing technology for high-throughput profiling of DNA methylations in Burkholderia pseudomallei.

Organism:

Burkholderia pseudomallei

Type:

Methylation profiling by high throughput sequencing

Platform:

GPL18301

2 Samples

Download data: CSV

(Submitter supplied) Burkholderia thailandensis is a soil-dwelling bacterium that shares many metabolic pathways with the ecologically similar, but evolutionarily distant, Pseudomonas aeruginosa. Among the diverse nutrients it can utilize is choline, which can be converted into the osmoprotectant glycine betaine and further catabolized as a source of carbon and nitrogen, similar to P. aeruginosa. Orthologs of genes in the choline catabolic pathway in these two bacteria showed distinct differences in gene arrangement as well as an additional orthologous transcriptional regulator in B. more...

Organism:

Burkholderia thailandensis E264

Type:

Expression profiling by high throughput sequencing

Platform:

GPL21888

16 Samples

Download data: XLSX

(Submitter supplied) Innate immunity responds to pathogens by producing alarm signals and activating pathways that make host cells inhospitable for pathogen replication. The intracellular bacterium Burkholderia thailandensis invades the cytosol, hijacks host actin, and induces cell fusion to spread to adjacent cells, forming multinucleated giant cells (MNGCs) which promotes bacterial replication. We show that type I interferon (IFN) restricts macrophage MNGC formation during B. more...

Organism:

Mus musculus

Type:

Expression profiling by array

Platform:

GPL23038

4 Samples

Download data: CEL

(Submitter supplied) Total transcript amplification (TTA) from single eukaryotic cells for transcriptome analysis is established, but TTA from a single prokaryotic cell presents additional challenges with much less starting material and lack of poly(A)-tails. We described, here, a novel method for single bacterium TTA, using a Burkholderia thailandensis model exposed to subinhibitory concentration of the antibacterial agent, glyphosate. more...

Organism:

Burkholderia thailandensis

Type:

Expression profiling by array

Platform:

GPL7113

3 Samples

Download data: TXT