GEO DataSets

(Submitter supplied) Two-component systems are widespread prokaryotic signal transduction devices which allow the regulation of cellular functions in response to changing environmental conditions. The two-component system DccRS (Cj1223-Cj1222) of Campylobacter jejuni is important for the colonization of chickens. Here we dissected the DccRS system in more detail and provide evidence that the sensor DccS selectively phosphorylates the cognate effector DccR. more...

Organism:

Campylobacter jejuni; Campylobacter jejuni subsp. jejuni 81116

Type:

Expression profiling by array

Platform:

GPL6315

6 Samples

Download data: GPR

(Submitter supplied) The Cj1223c gene was cloned downstream of a strong promoter into the replicating plasmid pRY108, and was highly expressed in the wild type Campylobacter jejuni 81-176 strain (overexpressed). The Cj1223c gene was knocked out by allelic replacement in the 81-176 strain background (mutant). This mutant also carried an empty pRY108 vector. The Cj1223c overexpressing strain and the mutant were grown overnight in liquid broth, supplemented with kanamycin. more...

Organism:

Campylobacter jejuni

Type:

Expression profiling by array

Platform:

GPL2782

8 Samples

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(Submitter supplied) In Campylobacter jejuni CmeR functions as a transcriptional repressor modulating the expression of the multidrug efflux pump CmeABC, which plays an important role in the resistance to antimicrobial agents and bile compounds. Using DNA microarray, we identified multiple genes that are either activated or repressed by CmeR in C. jejuni. The DNA microarray data was independently confirmed by quantitative real-time RT-PCR. more...

Organism:

Campylobacter jejuni

Type:

Expression profiling by array

Platform:

GPL532

6 Samples

Download data: TXT

(Submitter supplied) Background: During gut colonization, the enteric pathogen C. jejuni has to surmount the toxic effects of reactive oxygen species produced by its own metabolism, by the host immune system and by the intestinal microflora. Elucidation of C. jejuni oxidative stress defense mechanisms is critical for understanding Campylobacter pathophysiology. Results: The mechanisms of oxidative stress defenses in Campylobacter jejuni were characterized by transcriptional profiling, genes mutagenesis, and phenotypic analysis. more...

Organism:

Campylobacter jejuni; Campylobacter jejuni subsp. jejuni NCTC 11168 = ATCC 700819

Type:

Expression profiling by array

Platforms:

GPL7446 GPL6290

51 Samples

Download data: GPR

(Submitter supplied) Campylobacter jejuni is a leading cause of bacterial diarrheal disease and a frequent commensal of the intestinal tract of poultry and other animals. For optimal growth and colonization of hosts, C. jejuni employs two-component regulatory systems (TCSs) to monitor environmental conditions and promote proper expression of specific genes. We analyzed the potential of C. jejuni Cjj81176_1484 (Cjj1484) and Cjj81176_1483 (Cjj1483) to encode proteins of a cognate TCS that influences expression of genes possibly important for C. more...

Organism:

Campylobacter jejuni

Type:

Expression profiling by array

Platform:

GPL19897

18 Samples

Download data: TXT

(Submitter supplied) We perfomed the transcriptomic analysis of differential genetic expression in the CosR-knockdown condition with a DNA microarray. CosR-specific antisense-PNA was used for CosR-knockdown. The results revealed that CosR significantly affected the expression of several genes involved in various cellular function in Campylobacter. Final goal of this study is to figure out the importance and the role of CosR in Campylobacter by identification of the CosR regulons.

Organism:

Campylobacter jejuni

Type:

Expression profiling by array

Platform:

GPL15955

3 Samples

Download data: TXT

(Submitter supplied) C. jejuni, a spiral-shaped gram-negative bacterium, is a leading bacterial cause of human foodborne illness. Acute disease is associated with C. jejuni invasion of the intestinal epithelium. Further, maximal host cell invasion requires the secretion of proteins termed Campylobacter invasion antigens (Cia). As bile acids are known to alter the pathogenic behavior of other gastrointestinal pathogens, we hypothesized that the virulence potential of Campylobacter may be triggered by the bile acid deoxycholate (DOC). more...

Organism:

Campylobacter jejuni

Type:

Expression profiling by array

Platform:

GPL6315

6 Samples

Download data: GPR

(Submitter supplied) We examined two variants of the genome-sequenced strain, Campylobacter jejuni NCTC11168, which show marked differences in their virulence properties including colonization of poultry, invasion of Caco-2 cells, and motility. Transcript profiles obtained from whole genome DNA microarrays and proteome analyses demonstrated that these differences are reflected in late flagellar structural components and in virulence factors including those involved in flagellar glycosylation, and cytolethal distending toxin production. more...

Organism:

Campylobacter jejuni

Type:

Expression profiling by array

Datasets:

GDS976 GDS977

Platform:

GPL352

11 Samples

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Comparison of the V26 attenuated strain to the V1 strain with a component of the flagellar export apparatus FlhA inactivated. FlhA inactivation decreases virulence. Results show that the expression profile of V1 with FlhA inactivated is similar to that of V26.

Organism:

Campylobacter jejuni

Type:

Expression profiling by array, log ratio, 3 strain sets

Platform:

GPL352

Series:

GSE708

5 Samples

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Comparison of the V26 attenuated strain to the V1 strain with a component of the flagellar export apparatus FlhA inactivated. FlhA inactivation decreases virulence. Results show that the expression profile of V1 with FlhA inactivated is similar to that of V26.

Organism:

Campylobacter jejuni

Type:

Expression profiling by array, log ratio, 3 strain sets

Platform:

GPL352

Series:

GSE708

6 Samples

Download data

(Submitter supplied) In Neisseria gonorrhoeae, Fur (ferric uptake regulator) protein regulates iron homeostasis gene expression through binding to conserved sequences in promoters of iron-responsive genes. We have expanded the gonococcal Fur regulon using a custom microarray to monitor iron-responsive gene expression throughout the growth curve combined with a genome-wide in silico analysis to predict Fur boxes (FB), and in vivo FuRTA assays to detect genes able to bind Fur. more...

Organism:

Neisseria gonorrhoeae; Neisseria gonorrhoeae FA 1090; Neisseria meningitidis

Type:

Expression profiling by array

Platform:

GPL7320

24 Samples

Download data: CEL, TXT

(Submitter supplied) Campylobacter jejuni is a prevalent cause of bacterial gastroenteritis in humans worldwide. The mechanism by which C. jejuni survives stomach acidity remains unknown. Herein, we have demonstrated that C. jejuni with a fur deletion was more sensitive to acid than the wild-type strain. Profiling the acid stimulon of the C. jejuni ∆fur mutant allowed us to uncover Fur-regulated genes under acidic conditions. more...

Organism:

Campylobacter jejuni

Type:

Expression profiling by array

Platform:

GPL6293

27 Samples

Download data: CSV

(Submitter supplied) Comparing transciptional profile of a M. smegmatis mc2155 ΔsigF strain and wild type (control) in in vitro culture at two time points (exponential phase and stationary phase) for identification of SigF-dependent genes.

Organism:

Mycolicibacterium smegmatis

Type:

Expression profiling by array

Platform:

GPL5862

8 Samples

Download data: TXT

(Submitter supplied) Transcriptional regulation mediates adaptation of pathogens to environmental stimuli and is important for host colonisation. The Campylobacter jejuni genome sequence reveals a surprisingly small set of regulators, mostly of unknown function, suggesting an intricate regulatory network. Interestingly, C. jejuni lacks the homologues of ubiquitous regulators involved in stress response found in many other Gram-negative bacteria. more...

Organism:

Campylobacter jejuni

Type:

Expression profiling by array

Platform:

GPL6293

21 Samples

Download data: CSV

(Submitter supplied) Campylobacter jejuni is a highly prevalent food-borne pathogen that causes diarrhoeal disease in humans. A natural zoonotic, it must overcome significant stresses both in vivo and during transmission despite the absence of several traditional stress response genes. Its ability to interact with and invade human intestinal epithelial cells closely correlates with virulence. A C. jejuni microarray-based screen revealed that several known virulence genes and several uncharacterized genes, including spoT, were rapidly upregulated during infection of human epithelial cells. more...

Organism:

Campylobacter jejuni

Type:

Expression profiling by array

Platform:

GPL2791

12 Samples

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(Submitter supplied) DksA is well-known for its regulatory role in the transcription of ribosomal RNA and genes involved in amino acid synthesis in many bacteria. DksA is also reported to control expression of virulence genes in pathogenic bacteria. Here, we elucidated the roles of the DksA-like protein (CJJ81176_0160, Cj0125c) in the pathogenesis of Campylobacter jejuni. Like in other bacteria, transcription of stable RNA was repressed by DksA under stressful conditions in C. more...

Organism:

Campylobacter jejuni; Campylobacter jejuni subsp. jejuni 81-176

Type:

Expression profiling by array

Platform:

GPL532

12 Samples

Download data: TXT

(Submitter supplied) Xylella fastidiosa is the etiologic agent of a wide range of plant diseases including citrus variegated chlorosis (CVC), a major threat to the Brazilian citrus industry. Genome sequences of several strains of this phytopathogen are accessible, enabling large-scale functional studies. Transcript levels in different iron availabilities were assessed with DNA microarrays representing 2608 (91.6%) coding sequences (CDS) of X. more...

Organism:

Xylella fastidiosa

Type:

Expression profiling by array

Platform:

GPL2708

22 Samples

Download data

(Submitter supplied) Xylella fastidiosa is the etiologic agent of a wide range of plant diseases including citrus variegated chlorosis (CVC), a major threat to the Brazilian citrus industry. Genome sequences of several strains of this phytopathogen are accessible, enabling large-scale functional studies. Transcript levels in different iron availabilities were assessed with DNA microarrays representing 2608 (91.6%) coding sequences (CDS) of X. more...

Organism:

Xylella fastidiosa

Type:

Expression profiling by array

Platform:

GPL2708

22 Samples

Download data

(Submitter supplied) This SuperSeries is composed of the SubSeries listed below.

Organism:

Campylobacter jejuni subsp. jejuni NCTC 11168 = ATCC 700819; Campylobacter jejuni

Type:

Expression profiling by array

Platform:

GPL532

5 Samples

Download data: TXT

(Submitter supplied) Batch cultures of Wild-type C. jejuni NCTC 11168 were grown in 200 ml volumes of Mueller-Hinton broth in 250 ml baffled flasks. Microaerophilic conditions were generated using a MACS-VA500 microaerophilic work station (10 % Oxygen, 10 % Carbon dioxide, 80 % Nitrogen) from Don Whitley Scientific, Ltd which also maintained the growth temperature at 42 ºC. When mid-exponential phase was reached 0.010 mM NOC-5 & NOC-7 was added to one of the cultures. more...

Organism:

Campylobacter jejuni subsp. jejuni NCTC 11168 = ATCC 700819; Campylobacter jejuni

Type:

Expression profiling by array

Platform:

GPL532

4 Samples

Download data: TXT