GEO DataSets

(Submitter supplied) Investigation of whole genome changes in six serotypes of Actinobacillus pleuropneumoniae in control cultures compared to bacteria grown in media containg the iron chelator 2,2'-dipyridyl.

Organism:

Actinobacillus pleuropneumoniae serovar 1 str. 4074; Actinobacillus pleuropneumoniae serovar 3; Actinobacillus pleuropneumoniae serovar 5; Actinobacillus pleuropneumoniae; Actinobacillus pleuropneumoniae serovar 7; Actinobacillus pleuropneumoniae serovar 2 str. 4226; Actinobacillus pleuropneumoniae serovar 6

Type:

Expression profiling by array

Platform:

GPL11003

48 Samples

Download data: XYS

(Submitter supplied) native array description file: 090423_DTU_Actino_xRNA (for array no. 355074A) native array description file: 091013_DTU_Actino_xRNA (for arrays no. 382804A, 382816A, and 431045A) Protocol: High-density DNA array prepared with Maskless Array Synthesizer (MAS) technology. See manufacturer's website at http://www.nimblegen.com/.

Organism:

Actinobacillus pleuropneumoniae

2 Series

123 Samples

Download data: NDF

(Submitter supplied) To better understand effects of iron starvation on A. pleuropneumoniae and to identify new potential vaccine targets, we conducted transcript profiling using a DNA microarray containing all 2025 ORFs of the genome of A. pleuropneumoniae serotype 5b strain L20. Upon comparing growth of this pathogen in iron-sufficient versus iron-depleted medium, 210 genes were identified as being differentially expressed. more...

Organism:

Actinobacillus pleuropneumoniae

Type:

Expression profiling by array

Platform:

GPL3648

4 Samples

Download data: MEV

(Submitter supplied) To better understand effects of iron starvation on A. pleuropneumoniae and to identify new potential vaccine targets, we conducted transcript profiling using a DNA microarray containing all 2025 ORFs of the genome of A. pleuropneumoniae serotype 5b strain L20. Upon comparing growth of this pathogen in iron-sufficient versus iron-depleted medium, 210 genes were identified as being differentially expressed. more...

Organism:

Actinobacillus pleuropneumoniae

Type:

Expression profiling by array

Platform:

GPL3648

9 Samples

Download data: TXT

(Submitter supplied) Host-pathogen interactions are of great importance in understanding the pathogenesis of infectious microorganisms. We developed in vitro models to study porcine respiratory tract pathogens using an immortalized epithelial cell line, the St. Jude Porcine Lung (SJPL) cell line. In conditions where cytotoxicity was absent or low, the transcriptomic profile of A. pleuropneumoniae after contact with the porcine lung epithelial cells was determined using DNA microarrays. more...

Organism:

Actinobacillus pleuropneumoniae

Type:

Expression profiling by array

Platform:

GPL3648

8 Samples

Download data: MEV

(Submitter supplied) This SuperSeries is composed of the SubSeries listed below.

Organism:

Actinobacillus pleuropneumoniae serovar 5b str. L20; Actinobacillus pleuropneumoniae

Type:

Expression profiling by array

Platform:

GPL6658

16 Samples

Download data: MEV

(Submitter supplied) The transcriptome of Actinobacillus pleuropneumoniae dripbiofilm biofilms was compared to the transcriptome of effluent cells

Organism:

Actinobacillus pleuropneumoniae serovar 5b str. L20; Actinobacillus pleuropneumoniae

Type:

Expression profiling by array

Platform:

GPL6658

4 Samples

Download data: MEV

(Submitter supplied) The transcriptome of Actinobacillus pleuropneumoniae 4h static biofilms was compared to the transcriptome of 6h static biofilm

Organism:

Actinobacillus pleuropneumoniae serovar 5b str. L20; Actinobacillus pleuropneumoniae

Type:

Expression profiling by array

Platform:

GPL6658

4 Samples

Download data: MEV

(Submitter supplied) The transcriptome of Actinobacillus pleuropneumoniae biofilms was compared to the transcriptome of planktonic bacteria

Organism:

Actinobacillus pleuropneumoniae serovar 5b str. L20; Actinobacillus pleuropneumoniae

Type:

Expression profiling by array

Platform:

GPL6658

4 Samples

Download data: MEV

(Submitter supplied) The transcriptome of Actinobacillus pleuropneumoniae biofilms was compared to the transcriptome of planktonic bacteria

Organism:

Actinobacillus pleuropneumoniae serovar 5b str. L20; Actinobacillus pleuropneumoniae

Type:

Expression profiling by array

Platform:

GPL6658

4 Samples

Download data: MEV

(Submitter supplied) Bacteria can actively respond to host stress hormones (catecholamines), thereby regulate their growth, metabolism, virulence and other behaviors. This phenomenon provides new explanations of the fact that stress can influence the occurrence and development of infectious disease. Actinobacillus pleuropneumoniae is an important swine respiratory pathogen which has caused great economic losses worldwide. more...

Organism:

Actinobacillus pleuropneumoniae serovar 1 str. 4074; Actinobacillus pleuropneumoniae

Type:

Expression profiling by array

Platform:

GPL8394

15 Samples

Download data: TXT

(Submitter supplied) LuxS is an enzyme involved in the activated methyl cycle and the by-product autoinducer 2 (AI-2) was a quorum sensing signal in some species. In our previous study, the functional LuxS in AI-2 production was verified in the porcine respiratory pathogen Actinobacillus pleuropneumoniae. Enhanced biofilm formation and reduced virulence were observed in the luxS mutant. To comprehensively understand the luxS function, in this study, the transcriptional profiles were compared between the A. more...

Organism:

Actinobacillus pleuropneumoniae

Type:

Expression profiling by array

Platform:

GPL9691

16 Samples

Download data: TXT

(Submitter supplied) To study hepatic gene expression differences, liver samples from infected pigs (n = 10) were compared with liver samples from the non-infected control group (n = 5). Ten microarrays were performed, such that 5 randomly selected cDNA samples from the infected group were labeled with Oyster 550 (Genisphere Inc., Hatfield, PA, USA) and compared directly to 5 control animals labeled with Oyster 650 (Genisphere) analysing a pair of samples (one from an infected animal and one from a randomly chosen non-infected animal) on each microarray slide. more...

Organism:

Sus scrofa

Type:

Expression profiling by array

Platform:

GPL6849

10 Samples

Download data: GPR

(Submitter supplied) Actinobacillus pleuropneumoniae, the causative agent of porcine pleuropneumonia, is a highly contagious respiratory pathogen that causes severe losses to the swine industry worldwide. Current commercially-available vaccines are of limited value because they do not induce cross-serovar immunity and do not prevent development of the carrier state. Microarray-based comparative genomic hybridizations (M-CGH) were used to estimate whole genomic diversity of representative Actinobacillus pleuropneumoniae strains. more...

Organism:

Actinobacillus pleuropneumoniae; Actinobacillus pleuropneumoniae serovar 5b str. L20

Type:

Genome variation profiling by array

Platform:

GPL6658

74 Samples

Download data: MEV

(Submitter supplied) Microarray-based comparative genomic hybridizations were used to estimate whole genomic diversity of representative Actinobacillus pleuropneumoniae strains. Our goal was to identify conserved genes, especially those predicted to encode outer membrane proteins and lipoproteins because of their potential for the development of more effective vaccines. Keywords: Comparative Genomic Hybridisation

Organism:

Actinobacillus pleuropneumoniae; Actinobacillus pleuropneumoniae serovar 5b str. L20

Type:

Genome variation profiling by array

Platform:

GPL6658

33 Samples

Download data: TXT

(Submitter supplied) Bacteria can use host hormones as environment cue to modulate their pathogenic processes, which was discovered to play essential role in disease development. Actinobacillus pleuropneumoniae is one of the most important porcine respiratory pathogens causing great economic losses in the pig industry worldwide. Stress factors were found to contribute to the outcome of A. pleuropneumoniae infection. To test whether A. more...

Organism:

Actinobacillus pleuropneumoniae; Actinobacillus pleuropneumoniae serovar 1 str. 4074

Type:

Expression profiling by array

Platform:

GPL9691

6 Samples

Download data: TXT

(Submitter supplied) A. baumannii ATCC 17978 cells were incubated under iron replete (mueller-hinton) and iron limiting (MH + 200 µM 2,2'-dipyridyl) conditions, total RNA was extracted when cultures reached OD600=0.7.

Organism:

Acinetobacter baumannii

Type:

Expression profiling by array

Platform:

GPL11113

4 Samples

Download data: TXT

(Submitter supplied) Background: Haemophilus influenzae has an absolute aerobic growth requirement for heme and has developed multiple complex mechanisms to obtain this essential nutrient. Although an understanding of the heme acquisition mechanisms of H. influenzae is emerging, significant gaps remain to be elucidated. In a previous study we utilized H. influenzae strain Rd KW20 to demonstrate the utility of transcriptional profiling in defining the genes exhibiting altered transcription in response to environmental iron and heme levels. more...

Organism:

Haemophilus influenzae R2866; Haemophilus influenzae

Type:

Expression profiling by array

Platform:

GPL6594

12 Samples

Download data: TXT

(Submitter supplied) Background: Haemophilus influenzae has an absolute aerobic growth requirement for heme and has developed multiple complex mechanisms to obtain this essential nutrient. Although an understanding of the heme acquisition mechanisms of H. influenzae is emerging, significant gaps remain to be elucidated. In a previous study we utilized H. influenzae strain Rd KW20 to demonstrate the utility of transcriptional profiling in defining the genes exhibiting altered transcription in response to environmental iron and heme levels. more...

Organism:

Haemophilus influenzae

Type:

Expression profiling by array

Platform:

GPL6594

6 Samples

Download data: TXT

(Submitter supplied) Background: Haemophilus influenzae has an absolute aerobic growth requirement for heme and has developed multiple complex mechanisms to obtain this essential nutrient. Although an understanding of the heme acquisition mechanisms of H. influenzae is emerging, significant gaps remain to be elucidated. In a previous study we utilized H. influenzae strain Rd KW20 to demonstrate the utility of transcriptional profiling in defining the genes exhibiting altered transcription in response to environmental iron and heme levels. more...

Organism:

Haemophilus influenzae R2866; Haemophilus influenzae

Type:

Expression profiling by array

Platform:

GPL6594

6 Samples

Download data: TXT