GEO DataSets

(Submitter supplied) Glucocorticoids (GCs) have a long history of use as therapeutic agents for numerous skin diseases. Surprisingly, their specific molecular effects are largely unknown. To characterize GC action in epidermis, we compared the transcriptional profiles of primary human keratinocytes untreated and treated with dexamethasone (DEX) for 1, 4, 24, 48 and 72 hours using large-scale microarray analyses. The majority of genes were found regulated only after 24 hours and remained regulated throughout the treatment. more...

Organism:

Homo sapiens

Type:

Expression profiling by array

Dataset:

GDS5208

Platform:

GPL8300

20 Samples

Download data: CEL

Analysis of cultured epidermal keratinocytes treated with the synthetic glucocorticoid (GC) dexamethasone for up to 72 hours. GCs are used as therapeutic agents for skin conditions. Results provide insight into the molecular response of the epidermis to GCs.

Organism:

Homo sapiens

Type:

Expression profiling by array, transformed count, 2 agent, 5 time sets

Platform:

GPL8300

Series:

GSE26487

20 Samples

Download data: CEL

(Submitter supplied) Targets of Retinoic Acid (RA) were identified in primary human epidermal keratinocytes grown in the presence or absence of all-trans retinoic acid for 1, 4, 24, 48 and 72 hours. Targets of Thyroid Hormone (T3) were identified in primary human epidermal keratinocytes grown in the presence or absence of the hormone; same controls as for RA.

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL571

15 Samples

Download data: CEL, CHP

(Submitter supplied) Interleukin-1 is a proinflammatory and immunomodulatory cytokine that plays a crucial role in inflammatory diseases of the skin, including bacterial infections, bullous diseases, UV damage and especially psoriasis. To characterize the molecular effects of IL-1 in epidermis, we defined the transcriptional changes in human epidermal keratinocytes 1, 4, 24, and 48 h after treatment with IL-1a. IL-1 significantly regulated 388 genes, including genes associated with proteolysis, adhesion, signal transduction, proliferation, and epidermal differentiation. more...

Organism:

Homo sapiens

Type:

Expression profiling by array

Dataset:

GDS3011

Platform:

GPL571

8 Samples

Download data: CEL, CHP

Analysis of epidermal keratinocytes at various time points up to 48 hours post-interleukin-1 (IL-1) treatment. IL-1 is a proinflammatory and immunomodulatory cytokine that plays a role in inflammatory diseases of the skin. Results provide insight into gene products that mediate the effects of IL-1.

Organism:

Homo sapiens

Type:

Expression profiling by array, count, 2 agent, 4 time sets

Platform:

GPL571

Series:

GSE9120

8 Samples

Download data: CEL, CHP

(Submitter supplied) Cultured epidermal keratinocyte controls used for IFNg, TNFa and IL1 treatment. Keywords: other

Organism:

Homo sapiens

Type:

Expression profiling by array

Dataset:

GDS1846

Platform:

GPL8300

9 Samples

Download data: CEL, EXP

Analysis of keratinocytes treated with interferon gamma (IFN-gamma) for up to 48 hours. IFN-gamma is a multifunctional, immunomodulatory cytokine with cell type-specific antiviral activities. Results provide insight into molecular processes regulated by IFN-gamma in keratinocytes.

Organism:

Homo sapiens

Type:

Expression profiling by array, count, 2 agent, 4 time sets

Platform:

GPL8300

Series:

GSE440

9 Samples

Download data: CEL, EXP

(Submitter supplied) Cultured keratinocytes treated with TNFa in the presence or absence of NFkB inhibitor; time course 1, 4, 24 & 48 hrs. Keywords = Epidermis Keywords = skin Keywords = keratinocytes Keywords = inflammation Keywords = NFkB Keywords = TNFa Keywords: time-course

Organism:

Homo sapiens

Type:

Expression profiling by array

Dataset:

GDS1289

Platform:

GPL8300

16 Samples

Download data: CEL, EXP

Analysis of epidermal keratinocytes treated with TNFalpha in the presence of 10 uM parthenolide, an NF-kappa B inhibitor. Cells examined at various time points up to 48 hours following TNFalpha treatment. Results identify NF-kappa B-dependent subset of TNFalpha-regulated genes.

Organism:

Homo sapiens

Type:

Expression profiling by array, count, 3 agent, 4 time sets

Platform:

GPL8300

Series:

GSE2489

16 Samples

Download data: CEL, EXP

(Submitter supplied) Both ephrins and their receptors are membrane bound, restricting their interactions to the sites of direct cell-to-cell interfaces. They are widely expressed, often co-expressed and regulate developmental processes, cell adhesion, motility, survival, proliferation, and differentiation. Both tumor suppressor and oncogene activities are ascribed to EFNs and Ephs in various contexts. A major conundrum regarding the EFN/Eph system concerns their large number and functional redundancy, given the promiscuous cross-activation of ligands and receptors and the overlapping intracellular signaling pathways. more...

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL571

6 Samples

Download data: CEL

(Submitter supplied) The AhR is a ligand activated transcription factor that may be important in normal skin physiology. We compared gene expression profiles between AhR Wt and AhR KO primary mouse keratinocyte cultures. We identified 391 genes that were differentially expressed with a 1.5 fold cutoff and p<.05, and identified the AhR as an important regulator of genes involved in normal epidermal differentiation.

Organism:

Mus musculus

Type:

Expression profiling by array

Platform:

GPL16570

7 Samples

Download data: CEL

(Submitter supplied) Background: Glucocorticoids (GCs) are widely used anti-inflammatory drugs. While useful in clinical practice, patients taking GCs often suffer from skeletal side effects including growth retardation and decreased bone quality in adults. On a physiological level, GCs have been implicated in the regulation of chondrogenesis and osteoblast differentiation, as well as maintaining homeostasis in cartilage and bone. more...

Organism:

Mus musculus

Type:

Expression profiling by array

Dataset:

GDS2802

Platform:

GPL1261

12 Samples

Download data: CEL, CHP, EXP

Analysis of primary cultured fetal chondrocytes treated with pharmacological doses of dexamethasone (DEX) for 6 and 24 hours. DEX is a synthetic glucorticoid (GC) receptor agonist. Results provide insight into the effects of pharmacological GC on chondrocytes.

Organism:

Mus musculus

Type:

Expression profiling by array, count, 2 agent, 2 time sets

Platform:

GPL1261

Series:

GSE7683

12 Samples

Download data: CEL, CHP, EXP

(Submitter supplied) Disrupted skin barrier due to altered keratinocyte differentiation is common in pathologic conditions such as atopic dermatitis, ichthyosis and psoriasis. However, the molecular cascades governing keratinocyte terminal differentiation are still poorly understood. We have previously demonstrated that a dominant mutation in ZNF750 leads to a clinical phenotype that reminiscent of psoriasis and seborrheic dermatitis. more...

Organism:

Homo sapiens

Type:

Expression profiling by array

Dataset:

GDS4599

Platform:

GPL6244

6 Samples

Download data: CEL

Analysis of ZNF750-silenced HaCaT keratinocytes (KCs) at day 12 of calcium-induced differentiation. ZNF750 knockdown in HaCaT KCs leads to sustained cell proliferation and decreased apoptosis. Results provide insight into molecular mechanisms underlying keratinocyte terminal differentiation.

Organism:

Homo sapiens

Type:

Expression profiling by array, transformed count, 2 protocol sets

Platform:

GPL6244

Series:

GSE38039

6 Samples

Download data: CEL

(Submitter supplied) Covering denuded dermal surface after injury requires migration, proliferation and differentiation of skin keratinocytes. To clarify the major traits controlling these intermingled biological events, we surveyed the genomic modifications occurring during the course of a scratch closure of cultured human keratinocytes. Using a DNA microarray approach, we report the identification of 161 new markers of epidermal repair. more...

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL1521

36 Samples

Download data: TXT

(Submitter supplied) We hypothesized that treatment of the injured skin with occlusion limits TEWL and results in a phenotype of epithelial response that more closely resembles mucosa. Here we addressed whether different hydration conditions change gene expression patterns in epidermis using microarray study in rabbit partial-thickness incisional wound. Microarray on epidermis showed that global expression patterns of the genes in full occluded vs. more...

Organism:

Oryctolagus cuniculus

Type:

Expression profiling by array

Platform:

GPL11170

30 Samples

Download data: TXT

(Submitter supplied) In inflamed tissue, normal signal transduction pathways are altered by extracellular signals. For example, the JNK pathway is activated in psoriatic skin, which makes it an attractive target for treatment. To define comprehensively the JNK-regulated genes in human epidermal keratinocytes, we compared the transcriptional profiles of control and JNK inhibitor-treated keratinocytes, using DNA microarrays. more...

Organism:

Homo sapiens

Type:

Expression profiling by array

Dataset:

GDS2081

Platform:

GPL8300

8 Samples

Download data

Analysis of keratinocytes at various time points up to 48 hours following treatment with the Jun kinase (JNK) inhibitor SP600125. SP600125 is a reversible ATP-competitive inhibitor specific for the three isoforms of JNK. Results identify JNK-regulated genes in epidermal keratinocytes.

Organism:

Homo sapiens

Type:

Expression profiling by array, count, 2 agent, 4 time sets

Platform:

GPL8300

Series:

GSE4828

8 Samples

Download data

(Submitter supplied) We want to identifiy microRNAs modulated by aging in human primary keratinocytes prepared from skin samples from individuals of various age

Organism:

Homo sapiens; synthetic construct

Type:

Non-coding RNA profiling by array

Platform:

GPL21572

12 Samples

Download data: CEL, CHP