GEO DataSets

(Submitter supplied) Prochlorococcus contributes significantly to ocean primary productivity. The link between primary productivity and iron in specific ocean regions is well established and iron limitation of Prochlorococcus cell division rates in these regions has been shown. However, the extent of ecotypic variation in iron metabolism among Prochlorococcus and the molecular basis for differences is not understood. Here, we examine the growth and transcriptional response of Prochlorococcus strains, MED4 and MIT9313, to changing iron concentrations. more...

Organism:

Tiamatvirus PSSP7; Prochlorococcus marinus str. MIT 9313; Prochlorococcus marinus subsp. pastoris str. CCMP1986; Prochlorococcus phage P-SSM4

Type:

Expression profiling by array

Platform:

GPL11412

39 Samples

Download data: CEL

(Submitter supplied) Prochlorococcus is a genus of abundant and ecologically important marine cyanobacteria. Here, we present the comprehensive comparison of the structure and composition of the transcriptomes of two closely related Prochlorococcus strains, which, despite their similarities, have adapted their gene pool to specific environmental constraints. We used large-scale strand-specific cDNA sequencing, microarray RNA profiling and computational analyses to characterize the transcriptomes of Prochlorococcus sp. more...

Organism:

Prochlorococcus marinus subsp. pastoris str. CCMP1986; Tiamatvirus PSSP7; Prochlorococcus phage P-SSM4; Prochlorococcus marinus str. MIT 9313

Type:

Expression profiling by array

Platform:

GPL8853

5 Samples

Download data: CEL, TXT

(Submitter supplied) RNA-Seq technique was used to obtain the transcriptome map of Prochlorococcus MED4, including operons, untranslated regions, non-coding RNAs, and novel genes. Genome-wide expression profiles revealed that three factors contribute to core genome stabilization. First, a negative correlation between gene expression levels and protein evolutionary rates was observed. Highly expressed genes were overrepresented in the core genome but not in the flexible genome. more...

Organism:

Prochlorococcus marinus subsp. pastoris str. CCMP1986

Type:

Expression profiling by high throughput sequencing

Platform:

GPL17528

10 Samples

Download data: TXT

(Submitter supplied) In summer 2014, we conducted experiments to determine the effects of different N substrates on phytoplankton communities in the North Pacific Ocean and in the transition zone of the California Current and gyre (Shilova, Mills et al., 2017). Samples were incubated with nitrate, ammonium, urea, and filtered deep water (FDW) for 48 hours (T48). Two treatments added iron, alone (Fe) or with a mix of N substrates (N+Fe), to determine the effects of Fe on the utilization of N substrates. more...

Organism:

uncultured marine microorganism

Type:

Expression profiling by array

Platform:

GPL24371

23 Samples

Download data: TXT

(Submitter supplied) We examine how the transcriptome of Prochlorococcus strain NATL2A changes in the presence of a naturally co-occurring heterotroph, Alteromonas macleodii MIT1002. Significant changes in the Prochlorococcus transcriptome were evident within six hours of co-culture, with groups of transcripts changing in different temporal waves. Many transcriptional changes persisted throughout the 48-hour experiment, indicating that the presence of the heterotroph affected a stable shift in Prochlorococcus physiology. more...

Organism:

Alteromonas macleodii; Prochlorococcus marinus str. NATL2A

Type:

Expression profiling by high throughput sequencing

Platforms:

GPL20970 GPL20972

42 Samples

Download data: TXT

(Submitter supplied) Carbon fixation plays a central role in determining cellular redox poise, increasingly understood to be a key parameter in cyanobacterial physiology. In the cyanobacterium Prochlorococcus--—the most abundant phototroph in the oligotrophic oceans--—the carbon-concentrating mechanism (CCM) is reduced to the bare essentials. Given the ability of Prochlorococcus populations to grow under a wide range of oxygen concentrations in the ocean, we wondered how carbon and oxygen physiology intersect in this minimal phototroph. more...

Organism:

Prochlorococcus marinus subsp. pastoris str. CCMP1986

Type:

Expression profiling by array

Platform:

GPL19747

40 Samples

Download data: CEL