GEO DataSets

(Submitter supplied) Previous studies have shown that ischemia alters gene expression in normal and malignant tissues. There are no studies that evaluated effects of ischemia in renal tumors. This study examines the impact of ischemia and tissue procurement conditions on RNA integrity and gene expression in renal cell carcinoma. We used microarray analysis to evaulate the effect of ischemia and tissue procurement conditions on the gene expression changes in renal cell carcinoma

Organism:

Homo sapiens

Type:

Expression profiling by array

Dataset:

GDS4547

Platform:

GPL570

140 Samples

Download data: CEL

Analysis of ten renal tumors that were resected then immediately frozen or stored at 4°C, 22°C, 37°C for up to 240 minutes. Results provide insight into the impact of tissue acquisition method on tumor gene expression profiles.

Organism:

Homo sapiens

Type:

Expression profiling by array, transformed count, 10 specimen, 4 temperature, 6 time sets

Platform:

GPL570

Series:

GSE41137

140 Samples

Download data: CEL

(Submitter supplied) Time series of eleven breast cancer samples subjected to different cold ischemic stress of up to 3 hr post tumor excision. A different 2x2 factorial within this study evaluated the effect of stabilization method (RNAlater vs snap freezing) and stablization delay (0 and 40 min) at room temperature.

Organism:

Homo sapiens

Type:

Expression profiling by array

Dataset:

GDS4088

Platform:

GPL96

86 Samples

Download data: CEL

Analysis of breast tumor samples preserved using 2 different RNA stabilization methods (RNAlater or snap freezing) up to 3 hours post tumor excision. Results provide insight into the effects of tissue handling on RNA integrity and microarray measurements from resected breast cancers.

Organism:

Homo sapiens

Type:

Expression profiling by array, transformed count, 11 individual, 35 other, 2 protocol, 6 time sets

Platform:

GPL96

Series:

GSE25011

86 Samples

Download data: CEL

(Submitter supplied) In order to address the progression, metastasis, and clinical heterogeneity of renal cell cancer (RCC), transcriptional profiling with oligonucleotide microarrays (22,283 genes) was done on 49 RCC tumors, 20 non-RCC renal tumors, and 23 normal kidney samples. Samples were clustered based on gene expression profiles and specific gene sets for each renal tumor type were identified. Gene expression was correlated to disease progression and a metastasis gene signature was derived. more...

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL96

92 Samples

Download data: CEL, TXT

(Submitter supplied) Piwi-interacting RNAs (piRNAs) are a distinct group of small non-coding RNAs (sncRNAs) that silence transposable genetic elements to protect genome integrity. Due to their limited expression in gonads and sequence diversity, piRNAs remain the most mysterious class of small RNAs. Studies have shown piRNAs are present in somatic cells and dysregulated in gastric, breast and liver cancers. By deep sequencing 24 frozen benign kidney and clear cell renal cell carcinoma (ccRCC) specimens and using the publically available piRNA database, we found 26,991 piRNAs present in human kidney tissue. more...

Organism:

Homo sapiens

Type:

Non-coding RNA profiling by high throughput sequencing

Platform:

GPL10999

24 Samples

Download data: TXT

(Submitter supplied) Renal cell carcinoma comprises a variety of entities, the most common being the clear-cell, papillary and chromophobe subtypes. These subtypes are related to different clinical evolution; however, most therapies have been developed for clear-cell carcinoma and there is not a specific treatment based on different subtypes. In this study, one hundred and sixty-four paraffin samples from primary nephrectomies for localized tumors were analyzed. more...

Organism:

Homo sapiens

Type:

Non-coding RNA profiling by array

Platform:

GPL20907

164 Samples

Download data: TXT

(Submitter supplied) The understanding of metastatic spread is limited and molecular mechanisms causing particular characteristics of metastasis are largely unknown. This comprises the extremely varying dormancy periods of tumor cells in the secondary organ after metastatic spread, represented by the disease-free survival (DFS) of the patients, or differing numbers of metastases in different patients. Knowing the molecular fundamentals of these phenomena would support the individual prediction of patients´ outcome and facilitate the decision for an appropriate monitoring and therapy regime. more...

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL570

68 Samples

Download data: CEL

(Submitter supplied) We have sampled several tumour regions from a single ccRCC patient after 6 weeks everolimus treatment, including both primary and metastatic site samples to investigate intra-tumour heterogeneity.

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL6244

9 Samples

Download data: CEL

(Submitter supplied) Whole genome expression of 39 clear-cell renal cell carcinomas tumors

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL1708

39 Samples

Download data: TXT

(Submitter supplied) Formalin-fixed, paraffin-embedded (FFPE) tissues are an underused resource for molecular analyses. This proof of concept study aimed to compare RNAseq results from FFPE biopsies with the corresponding RNAlater® (Qiagen, Germany) stored samples from clear cell renal cell carcinoma (ccRCC) patients, to enable the decision of whether or not it is feasible to apply RNAseq technology to archival tissue. Core biopsies were obtained with a 16g needle from 16 patients undergoing partial or full nephrectomy. The RNA sequencing libraries were generated applying the new Illumina TruSeq® Access library preparation protocol.

Organism:

Homo sapiens

Type:

Expression profiling by high throughput sequencing

Platform:

GPL16791

64 Samples

Download data: TXT

(Submitter supplied) Each total RNA sample is hybridized to two different arrays: Affymetrix U133A (GPL96) and U133B (GPL97). For most of the normal tissue samples there is a renal clear cell carcinoma sample from the same patient. There is no matching tumor sample for normal sample N1. For most of the renal clear cell carcinoma samples there is a corresponding adjacent normal tissue sample from the same patient. There are no matching normal tissue samples for C011 or C032. Keywords = kidney Keywords = renal Keywords = RCC Keywords = carcinoma Keywords = cancer Keywords: parallel sample

Organism:

Homo sapiens

Type:

Expression profiling by array

Datasets:

GDS505 GDS507

Platforms:

GPL96 GPL97

34 Samples

Download data: CEL

Investigation into mechanisms of renal clear cell carcinogenesis (RCC). Comparison of renal clear cell tumor tissue and adjacent normal tissue isolated from the same surgical samples.

Organism:

Homo sapiens

Type:

Expression profiling by array, count, 2 disease state, 10 individual sets

Platform:

GPL97

Series:

GSE781

17 Samples

Download data: CEL

Investigation into mechanisms of renal clear cell carcinogenesis (RCC). Comparison of renal clear cell tumor tissue and adjacent normal tissue isolated from the same surgical samples.

Organism:

Homo sapiens

Type:

Expression profiling by array, count, 2 disease state, 10 individual sets

Platform:

GPL96

Series:

GSE781

17 Samples

Download data: CEL

(Submitter supplied) Background: Clear cell renal cell carcinomas (ccRCCs) display divergent clinical phenotypes. Up to one third of patients with local disease at diagnosis will progress. Therefore, it is a need to identify patients at risk for recurrence. In the present study, we analyzed genome-wide promoter DNA methylation at diagnosis in relation to clinicopathological parameters, in order to identify DNA methylation profiles associated with risk for progress. more...

Organism:

Homo sapiens

Type:

Methylation profiling by array

Platform:

GPL13534

144 Samples

Download data: TXT

(Submitter supplied) Clear cell papillary renal cell carcinoma (CCPRCC) is a low-grade renal neoplasm with morphological characteristics mimicking both clear cell renal cell carcinoma (CCRCC) and papillary renal cell carcinoma (PRCC). However, despite some overlapping features, their morphological, immunohistochemical, and molecular profiles are distinct. To better understand the biology of this tumor, we analyze the miRNA expression profiles of a set of CCPRCC by microarrays.

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL16770

8 Samples

Download data: TXT