GEO DataSets

(Submitter supplied) Compartment-specific control of gene expression during Bacillus subtilis sporulation is governed by a cascade of four sigma factors, sigmaF and sigmaG in the forespore and sigmaE and sigmaK in the mother cell. In this work, we combined transcriptional analyses and transcriptional start site mapping to define the sigmaF, sigmaE, sigmaG and sigmaK regulons in Clostridium difficile. A total of about 225 genes were under the control of these sigma factors: 25 in the sigmaF regulon, 97 sigmaE-dependent genes, 50 sigmaG-governed genes and 56 genes specifically controlled by sigmaK. more...

Organism:

Clostridioides difficile

Type:

Expression profiling by array

Platform:

GPL10556

4 Samples

Download data: GPR, TXT, XLS

(Submitter supplied) Compartment-specific control of gene expression during Bacillus subtilis sporulation is governed by a cascade of four sigma factors, sigmaF and sigmaG in the forespore and sigmaE and sigmaK in the mother cell. In this work, we combined transcriptional analyses and transcriptional start site mapping to define the sigmaF, sigmaE, sigmaG and sigmaK regulons in Clostridium difficile. A total of about 225 genes were under the control of these sigma factors: 25 in the sigmaF regulon, 97 sigmaE-dependent genes, 50 sigmaG-governed genes and 56 genes specifically controlled by sigmaK. more...

Organism:

Clostridioides difficile

Type:

Expression profiling by array

Platform:

GPL10556

2 Samples

Download data: GPR, TXT, XLS

(Submitter supplied) This SuperSeries is composed of the SubSeries listed below.

Organism:

Clostridioides difficile

Type:

Expression profiling by array

Platform:

GPL10556

18 Samples

Download data: GPR

(Submitter supplied) Compartment-specific control of gene expression during Bacillus subtilis sporulation is governed by a cascade of four sigma factors, sigmaF and sigmaG in the forespore and sigmaE and sigmaK in the mother cell. In this work, we combined transcriptional analyses and transcriptional start site mapping to define the sigmaF, sigmaE, sigmaG and sigmaK regulons in Clostridium difficile. A total of about 225 genes were under the control of these sigma factors: 25 in the sigmaF regulon, 97 sigmaE-dependent genes, 50 sigmaG-governed genes and 56 genes specifically controlled by sigmaK. more...

Organism:

Clostridioides difficile

Type:

Expression profiling by array

Platform:

GPL10556

4 Samples

Download data: GPR, TXT, XLS

(Submitter supplied) Compartment-specific control of gene expression during Bacillus subtilis sporulation is governed by a cascade of four sigma factors, sigmaF and sigmaG in the forespore and sigmaE and sigmaK in the mother cell. In this work, we combined transcriptional analyses and transcriptional start site mapping to define the sigmaF, sigmaE, sigmaG and sigmaK regulons in Clostridium difficile. A total of about 225 genes were under the control of these sigma factors: 25 in the sigmaF regulon, 97 sigmaE-dependent genes, 50 sigmaG-governed genes and 56 genes specifically controlled by sigmaK. more...

Organism:

Clostridioides difficile

Type:

Expression profiling by array

Platform:

GPL10556

4 Samples

Download data: GPR, TXT, XLS

(Submitter supplied) Compartment-specific control of gene expression during Bacillus subtilis sporulation is governed by a cascade of four sigma factors, sigmaF and sigmaG in the forespore and sigmaE and sigmaK in the mother cell. In this work, we combined transcriptional analyses and transcriptional start site mapping to define the sigmaF, sigmaE, sigmaG and sigmaK regulons in Clostridium difficile. A total of about 225 genes were under the control of these sigma factors: 25 in the sigmaF regulon, 97 sigmaE-dependent genes, 50 sigmaG-governed genes and 56 genes specifically controlled by sigmaK. more...

Organism:

Clostridioides difficile

Type:

Expression profiling by array

Platform:

GPL10556

4 Samples

Download data: GPR, TXT, XLS

(Submitter supplied) Purpose: The goal of this study was to identify genes whose expression is induced during sporulation in a Spo0A-, σF-, σE-, σG-, and σK-dependent manner. Methods: Whole genome RNA sequencing was performed on wildtype, spo0A-, sigF-, sigE-, sigG-, and sigK- C. difficile strains (strain 630 background; JIR8094 = parent strain), and the transcriptional profiles of the different mutants during growth on 70:30 agar plates were determined using an Illumina HiSeq1000. more...

Organism:

Clostridioides difficile 630

Type:

Expression profiling by high throughput sequencing

Platform:

GPL18533

18 Samples

Download data: TXT

(Submitter supplied) Purpose: A goal of this study was to identify genes induced in the mother cell during Clostridium difficile sporulation (specifically in a σE-, σK-, and SpoIIID-dependent manner). Methods: Whole genome RNA sequencing was performed on wildtype, sigE-, sigK- and spoIIID- C. difficile strains (strain 630 background; JIR8094 = parent strain), and the transcriptional profiles of the different mutants during growth on 70:30 agar plates were determined using an Illumina MiSeq1000. more...

Organism:

Clostridioides difficile 630

Type:

Expression profiling by high throughput sequencing

Platform:

GPL19481

12 Samples

Download data: TXT

(Submitter supplied) This study is based on two microarray datasets, in one hand a phase transition comparison using the expression profiles of 630E strain after 4h and 10h of growth. In other hand a comparison at 10h of growth between a mutant of the sigH gene and the WT strains. This experimental procedure was designed to investigate the effect of sigH in the growth phase transition of Clostridium difficile. This SuperSeries is composed of the SubSeries listed below.

Organism:

Clostridioides difficile

Type:

Expression profiling by array

Platform:

GPL10556

8 Samples

Download data: GPR

(Submitter supplied) Transcriptionnal profiling of C. difficile 630E strain vs a sigH mutant after 10h of growth in TY

Organism:

Clostridioides difficile

Type:

Expression profiling by array

Platform:

GPL10556

4 Samples

Download data: GPR

(Submitter supplied) Transcriptional profiling of C. difficile 630E strain after 4h vs 10h of growth

Organism:

Clostridioides difficile

Type:

Expression profiling by array

Platform:

GPL10556

4 Samples

Download data: GPR

(Submitter supplied) In Gram-positive organisms the general stress response is regulated by the alternative sigma factor sigma B (σB). In C. difficile this sigma factor has been implicated in protection against stressors such as reactive oxygen species and antimicrobial compounds. Here, we show that an anti-σB antibody can be used to demonstrate overexpression of σB in C. difficile and that this overexpression is tolerated for short periods of time, despite its toxicity. more...

Organism:

Clostridioides difficile 630

Type:

Expression profiling by array

Platform:

GPL25054

16 Samples

Download data: TXT, XLS

(Submitter supplied) This series of experiments was designed to identify the program of gene transcription for a single differentiating cell type during sporulation in Bacillus subtilis. The mother cell is one of two cell types generated by asymmetric division of sporulating cells approximately two hours after initiation of sporulation. The program is governed by a hierarchical cascade consisting of the transcription factors: sigmaE, sigmaK, GerE, GerR (YlbO) and SpoIIID. more...

Organism:

Bacillus subtilis

Type:

Expression profiling by array

Platform:

GPL1353

15 Samples

Download data: TIFF

(Submitter supplied) Clostridium acetobutylicum, the endospore-forming anaerobe best known for its ABE (acetone-butanol-ethanol) fermentation, has received renewed attention recently for the biological production of butanol, both for bulk chemical production and as a potential biofuel. With butanol production in mind, most of the recent research on C. acetobutylicum has focused on increasing butanol production, tolerance to butanol, and optimizing it for various substrates. more...

Organism:

Clostridium acetobutylicum ATCC 824

Type:

Expression profiling by array

Platform:

GPL10908

24 Samples

Download data: TXT

(Submitter supplied) Genes encoding the alternative sigma factor SigmaB, involved in the general stress response in firmicutes, as well as its regulators are present in the genome of the enteropathogen Clostridium difficile. We inactivated the sigB gene using the ClosTron technology in order to identify the role of this sigma factor in the physiology of this bacterium. Transcriptomic experiments showed that SigB positively and negatively controlls several genes involved in different cellular processes such as metabolism, sporulation and stress response.

Organism:

Clostridioides difficile

Type:

Expression profiling by array

Platform:

GPL18319

4 Samples

Download data: GPR, TXT, XLS

(Submitter supplied) Transcriptional profiling of C. difficile 630E strain vs. a sig54 mutant after 6h of growth in TY.

Organism:

Clostridioides difficile

Type:

Expression profiling by array

Platform:

GPL10556

4 Samples

Download data: GPR, TXT, XLS

(Submitter supplied) This SuperSeries is composed of the SubSeries listed below.

Organism:

Clostridioides difficile

Type:

Expression profiling by array

Platforms:

GPL10556 GPL18319

8 Samples

Download data: GPR

(Submitter supplied) Transcriptional profiling of C. difficile 630E strain vs. an Hfq antisense after 7.5h of growth inTYt.

Organism:

Clostridioides difficile

Type:

Expression profiling by array

Platform:

GPL18319

4 Samples

Download data: GPR, TXT, XLS

(Submitter supplied) Clostridium difficile is an emergent human pathogen and the most common cause of nosocomial diarrhea. Our recent deep-sequencing data strongly suggest the importance of RNA-based mechanisms for the control of gene expression in C. difficile. The RNA chaperon protein Hfq is a key component of post-transcriptional regulatory network in various bacterial systems. In an effort to understand the function of Hfq in C. more...

Organism:

Clostridioides difficile

Type:

Expression profiling by array

Platform:

GPL10556

4 Samples

Download data: GPR, TXT, XLS

(Submitter supplied) Transcriptionnal profiling of C. difficile 630E strain vs a sigD mutant after 6h of growth in BHI

Organism:

Clostridioides difficile

Type:

Expression profiling by array

Platform:

GPL10556

4 Samples

Download data: GPR