GEO DataSets

(Submitter supplied) In bacteria and archaea, CRISPR loci confer adaptive, sequence-based immunity against viruses and plasmids. CRISPR interference is specified by CRISPR RNAs (crRNAs) that are transcribed and processed from CRISPR spacers and repeats. Pre-crRNA processing is essential for CRISPR interference in all systems studied thus far. Here we examine crRNA biogenesis and CRISPR interference in naturally competent Neisseria spp., including the human pathogen N. more...

Organism:

Neisseria lactamica 020-06

Type:

Expression profiling by high throughput sequencing

Platform:

GPL16715

2 Samples

Download data: TXT

(Submitter supplied) Neisseria meningitidis is a human commensal that occasionally causes life-threatening infections such as bacterial meningitis and septicemia. Despite experimental evidence that gene regulation as well as the expression of small non-coding RNAs (sRNAs) affect meningococcal virulence, the organization of its transcriptome, including in particular the biogenesis of sRNAs and their mode of action, is only poorly understood. more...

Organism:

Neisseria meningitidis 8013

Type:

Expression profiling by high throughput sequencing; Other

Platforms:

GPL22295 GPL22296

8 Samples

Download data: WIG

(Submitter supplied) Analysis of CRISPR RNAs from both total Pyrococcus furiosus RNA and those that co-purify with the Cmr complex, which has been predicted to be involved in RNA-mediated targeting of foreign RNAs in prokaryotes. Small RNAs (20 - 70 nts) were isolated either from total Pyrococcus furiosus RNA or RNAs isolated from immunopurified Cmr complexes. The RNAs were cloned and sequenced using the Illumina platform. more...

Organism:

Pyrococcus furiosus

Type:

Expression profiling by high throughput sequencing

Platform:

GPL14794

2 Samples

Download data: BEDGRAPH, SAM

(Submitter supplied) CRISPR-Cas immune systems function to defend prokaryotes against potentially harmful mobile genetic elements including viruses and plasmids. The multiple CRISPR-Cas systems (Types I, II, III) each recognize and target destruction of foreign invader nucleic acids via structurally and functionally diverse effector complexes (crRNPs). CRISPR-Cas effector complexes are comprised of CRISPR RNAs (crRNAs) that contain sequences homologous to the invading nucleic acids and Cas proteins specific to each immune system type. more...

Organism:

Pyrococcus furiosus

Type:

Non-coding RNA profiling by high throughput sequencing; Other

Platform:

GPL14794

4 Samples

Download data: BEDGRAPH

(Submitter supplied) Transcriptome sequencing was carried out on an Illumina HiSeq platform to investigate the activation of CRISPR-Cas and DNA repair systems by Csa3a in Sulfolobus islandicus Rey15A. We compared the differently expressed genes in Sulfolobus islandicus Rey15A strain with csa3a overexpression vs. Sulfolobus islandicus Rey15A strain carrying an empty expression vector, cas1 deletion strain with csa3a overexpression vs. more...

Organism:

Saccharolobus islandicus REY15A

Type:

Expression profiling by high throughput sequencing

Platforms:

GPL23497 GPL23498

12 Samples

Download data: XLS

(Submitter supplied) Neisseria meningitidis, a commensal β-proteobacterium of the human nasopharynx, constitutes a worldwide leading cause of sepsis and epidemic meningitis. The molecular basis for their "accidental" pathogenicity is still not fully understood. Here, we show that knock-out strains lacking the Cas9 protein are impaired in the adhesion to human nasopharyngeal cells which constitutes a central step in the pathogenesis of invasive meningococcal disease. more...

Organism:

Neisseria meningitidis 8013

Type:

Expression profiling by high throughput sequencing; Other

Platform:

GPL24628

8 Samples

Download data: WIG

(Submitter supplied) The CRISPR-Cas system represents an RNA-based adaptive immune response system in prokaryotes. CRISPRs (Clustered Regularly Interspaced Short Palindromic Repeats) consist of arrays of short repeat sequences interspaced by non-repetitive short spacers, some of which show sequence similarity to foreign phage genetic elements. Their cistronic transcripts are processed to produce the mature CRISPR RNAs (crRNAs), the elements that confer immunity by base-pairing with exogenous nucleic acids. more...

Organism:

Thermus thermophilus

Type:

Expression profiling by high throughput sequencing

Platforms:

GPL14992 GPL14993

6 Samples

Download data: TXT

(Submitter supplied) This study addresses the key role of the extracytoplasmic function (ECF) sigma factor DdvS in the Gram-negative bacterium Myxococcus xanthus. It allows us to determine its regulon and it shows that the expression of ddvS and its regulón depends on the global regulatory complex CarD-CarG.

Organism:

Myxococcus xanthus

Type:

Expression profiling by array

Platform:

GPL24768

8 Samples

Download data: TXT

(Submitter supplied) We have previously reported that Mycobacterium tuberculosis Rv2837c (cnpB) encodes a phosphodiesterase that specifically cleaves cyclic di-AMP (c-di-AMP) into AMP. Deletion of cnpB results in significant virulence attenuation in a mouse pulmonary infection model, which is very likely due to the significantly elevated c-di-AMP levels as overexpression of Mtb diadenylate cyclase, disA, also leads to a similar outcome. more...

Organism:

Mycobacterium tuberculosis

Type:

Expression profiling by high throughput sequencing

Platform:

GPL18768

6 Samples

Download data: XLSX