GEO DataSets

(Submitter supplied) Numerous studies have examined changes in transcript levels after Anopheles gambiae takes a blood meal. Marinotti et al. (2006) used microarrays and reported massive changes in transcript levels 3 h after feeding (BF3h) compared to non-blood fed (NBF). We were intrigued by the number of transcripts for structural cuticular proteins (CPs) that showed such major differences in levels and employed paired-end (50 bp) RNA-seq technology to compare whole body transcriptomes from 5-day-old females NBF and BF3h. more...

Organism:

Anopheles gambiae

Type:

Expression profiling by high throughput sequencing

Platform:

GPL17644

8 Samples

Download data: GTF

(Submitter supplied) With their genome sequenced, Anopheles gambiae mosquitoes now serve as a powerful tool for basic research in comparative, evolutionary and developmental biology. The knowledge generated by these studies is expected to reveal molecular targets for novel vector control and pathogen transmission blocking strategies. Comparisons of gene-expression profiles between adult male and nonblood-fed female Anopheles gambiae mosquitoes revealed that roughly 22% of the genes showed sex-dependent regulation. more...

Organism:

Plasmodium falciparum; Anopheles gambiae

Type:

Expression profiling by array

Platform:

GPL1321

36 Samples

Download data: CEL, CHP, EXP

(Submitter supplied) Background: The Anopheles gambiae salivary glands play a major role in malaria transmission and express a variety of bioactive components that facilitate blood-feeding by preventing platelet aggregation, blood clotting, vasodilatation, and inflammatory and other reactions at the probing site on the vertebrate host. Results: We have performed a global transcriptome analysis of the A. gambiae salivary gland response to blood-feeding, to identify candidate genes that are involved in hematophagy. more...

Organism:

Anopheles gambiae

Type:

Expression profiling by array

Platform:

GPL10753

3 Samples

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(Submitter supplied) Malaria is caused by Plasmodium parasites, which are transmitted via the bites of infected Anopheline mosquitoes. Midgut invasion is a major bottleneck for Plasmodium development inside the mosquito vectors as a rapidly responding immune system recognizes ookinetes and recruits killing factors from the midgut and surrounding tissues, dramatically reducing the population of invading ookinetes before they can successfully traverse the midgut epithelium. more...

Organism:

Anopheles gambiae

Type:

Expression profiling by high throughput sequencing

Platform:

GPL15693

18 Samples

Download data: TXT

(Submitter supplied) The female midgut compartment specifc transcriptomes have been determined with microarray analyses. Keywords: glass slide microarray analyses

Organism:

Anopheles gambiae

Type:

Expression profiling by array

Platform:

GPL4579

18 Samples

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(Submitter supplied) Purpose: Next-generation sequencing (NGS) has revolutionized systems-based analysis of cellular pathways. The goals of this study are to compare NGS-derived retinal transcriptome profiling (RNA-seq) to microarray and quantitative reverse transcription polymerase chain reaction (qRT–PCR) methods and to evaluate protocols for optimal high-throughput data analysis. Methods: Retinal mRNA profiles of 21-day-old wild-type (WT) and neural retina leucine zipper knockout (Nrl−/−) mice were generated by deep sequencing, in triplicate, using Illumina GAIIx. more...

Organism:

Mus musculus

Type:

Expression profiling by high throughput sequencing

Platform:

GPL11002

6 Samples

Download data: BAM, TXT, XLS

(Submitter supplied) Anopheles gambiae mosquitoes play an important role in malaria transmission. In sub-Saharan Africa, the dry season can last several months. The mechanisms for mosquito population to survive through the dry season are poorly understood. One possible mechanism is that adults increase their desiccation tolerance over the dry season. Genetic analyses have shown that inversions 2La, 2Rb, 2Rc, 2Rd and 2Ru are associated with aridity resistance, however little is known about the transcriptional response of genes in response to desiccation. more...

Organism:

Plasmodium falciparum; Anopheles gambiae

Type:

Expression profiling by array

Dataset:

GDS4552

Platform:

GPL1321

19 Samples

Download data: CEL

Analysis of adult females exposed to 30% (desiccated) relative humidity for up to 36 hrs. The An. gambiae malaria vector populations are observed year round but their densities are low during the dry season. Results provide insight into molecular basis of the malaria vector's desiccation response.

Organism:

Plasmodium falciparum; Anopheles gambiae

Type:

Expression profiling by array, count, 3 stress, 3 time sets

Platform:

GPL1321

Series:

GSE25433

19 Samples

Download data: CEL

(Submitter supplied) This SuperSeries is composed of the SubSeries listed below.

Organism:

Homo sapiens

Type:

Expression profiling by array; Expression profiling by high throughput sequencing

Platforms:

GPL13447 GPL10999

21 Samples

Download data: BEDGRAPH, CEL, TXT

(Submitter supplied) mRNA expression was profiled from pooled bronchial airway epithelial cell brushings (n=3 patients/pool) obtained during bronchoscopy from healthy never (NS) and current smokers (S) and smokers with (C) and without (NC) lung cancer

Organism:

Homo sapiens

Type:

Expression profiling by high throughput sequencing

Platform:

GPL10999

8 Samples

Download data: BEDGRAPH, GTF, TXT

(Submitter supplied) mRNA expression was assayed from bronchial epithelial cell samples from smokers with and without lung cancer. A subset of the samples (2 of the lung cancer samples and 3 of the no cancer samples) were pooled and underwent whole transcriptome sequencing. The goals were to compare whole transcriptome sequencing gene expression levels to gene expression levels derived from these samples run on the Affymetrix HGU133A 2.0 platform.

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL13447

13 Samples

Download data: CEL

(Submitter supplied) Anopheles gambiae, the primary African malarial mosquito, exhibits numerous behaviors that are under diel and circadian control, including locomotor activity, swarming, mating, host seeking, eclosion, egg laying and sugar feeding. However, little has been performed to elucidate the molecular basis for these daily rhythms. To study how gene expression is globally regulated by diel and circadian mechanisms, we have undertaken a DNA microarray analysis of A. more...

Organism:

Plasmodium falciparum; Anopheles gambiae

Type:

Expression profiling by array

Dataset:

GDS4261

Platform:

GPL1321

104 Samples

Download data: CEL

Analysis of heads and bodies of mated, non-blood-fed, adult females maintained under a 12hr:12hr light:dark (LD) cycle or constant dark (DD) conditions; samples were collected every 4hr for 48 hr. Results provide insight into molecular mechanisms under circadian and LD cycle control.

Organism:

Plasmodium falciparum; Anopheles gambiae

Type:

Expression profiling by array, transformed count, 2 growth protocol, 26 time, 2 tissue sets

Platform:

GPL1321

Series:

GSE22585

104 Samples

Download data: CEL

(Submitter supplied) Senescence is a biological phenomenon experienced by all living eukaryote organisms. Genome-wide gene expression associated with aging has been explored in model organisms such as Drosophila melanogaster and Caenorhabditis elegans, but this has not been well understood in African malaria vector, Anopheles gambiae. Gene expression profiling using DNA microarray allows for simultaneous study of changes in mRNA levels for thousands of genes. more...

Organism:

Plasmodium falciparum; Anopheles gambiae

Type:

Expression profiling by array

Platform:

GPL1321

31 Samples

Download data: CEL

(Submitter supplied) In spite of the many recent developments in the field of vector sialomics, the salivary glands of larvalmosquitoes have been largely unexplored. We used whole-transcriptome microarray analysis to create a gene-expression profile of the salivary gland tissue of fourth-instar Anopheles gambiae larvae, and compare it to the gene-expression profile of a matching group of whole larvae. We identified a total of 221 probes with expression values that were (a) significantly enriched in the salivary glands, and (b)sufficiently annotated as to allow the prediction of the presence/absence of signal peptides in their corresponding gene products. more...

Organism:

Plasmodium falciparum; Anopheles gambiae

Type:

Expression profiling by array

Platform:

GPL1321

6 Samples

Download data: CEL

(Submitter supplied) Seasonal photoperiodism is responsable in aphid for the switch from asexual to sexual reproduction. In order to identify genes regulating the photoperiodic response, a cDNA microarray from the pea aphid was used to compare RNA populations from short- and long-day reared aphids. Analyses revealed that 64 different transcripts were significantly regulated, with a strong biological signature for cuticular proteins and proteins involved in cellular signalling or signal transduction. more...

Organism:

Acyrthosiphon pisum

Type:

Expression profiling by array

Platform:

GPL4580

12 Samples

Download data

(Submitter supplied) Background: The mosquito Anopheles gambiae is a major vector of human malaria. Increasing evidence indicates that blood cells (hemocytes) comprise an essential arm of the mosquito innate immune response against both bacteria and malaria parasites. To further characterize the role of hemocytes in mosquito immunity, we undertook the first genome-wide transcriptomic analyses of adult female An. gambiae hemocytes following infection by two species of bacteria and a malaria parasite. more...

Organism:

Anopheles gambiae

Type:

Expression profiling by array

Platforms:

GPL8551 GPL8550 GPL8552

11 Samples

Download data

(Submitter supplied) The number of legacy chemicals without toxicity reference values combined with the rate of new chemical development are overwhelming the capacity of the traditional risk assessment paradigm. More efficient approaches are needed to quantitatively estimate chemical risks. In this study, rats were dosed orally with multiple doses of six chemicals for 5 days, 2, 4, and 13 weeks. Target organs were analyzed for traditional histological and organ weight changes and transcriptional changes using microarrays. more...

Organism:

Rattus norvegicus

Type:

Expression profiling by array

Platform:

GPL16985

720 Samples

Download data: CEL

(Submitter supplied) BACKGROUND: Idiopathic Pulmonary Fibrosis (IPF) is a lethal lung disease of unknown etiology. A major limitation in transcriptomic profiling of lung tissue in IPF has been a dependence on snap-frozen fresh tissues (FF). In this project we sought to determine whether RNA-Seq could be used to identify IPF expression profiles from archived Formalin-Fixed Paraffin-Embedded (FFPE) lung fibrotic tissue. RESULTS: We isolated total RNA from 7 IPF and 5 control FFPE lung tissues (median archived time 6 years) and performed 50 bp paired-end sequencing on Illumina 2000 HiSeq. more...

Organism:

Homo sapiens

Type:

Expression profiling by high throughput sequencing

Platform:

GPL11154

11 Samples

Download data: TXT