GEO DataSets

(Submitter supplied) We previously found a short sleeper mutant, fmn, and identified its mutation in the dopamine transporter gene. In an attempt to discover additional sleep related genes in Drosophila, we carried out a microarray analysis comparing mRNA expression in heads of fmn and control flies and found differentially expressed genes. Next, using the pan-neuronal Gal4 driver elav-Gal4 and UAS-RNA interference (RNAi) to knockdown individual genes, we performed a functional screen. more...

Organism:

Drosophila melanogaster

Type:

Expression profiling by array

Platform:

GPL1322

14 Samples

Download data: CEL, CHP

(Submitter supplied) To gain insight into the dynamic molecular processes that are altered during prolonged wakefulness and during sleep. We performed an RNA expression profiling study examining temporal changes in the brain of Drosophila in relationship to the duration of prior sleep or wakefulness. Our experimental design allowed us to determine whether genes identified as differentially regulated between sleep and wakefulness were up- or down-regulated in these states. more...

Organism:

Drosophila melanogaster

Type:

Expression profiling by array

Dataset:

GDS1686

Platform:

GPL72

30 Samples

Download data: CEL, EXP

Analysis of brain of Canton-S females deprived of sleep by perturbations during their normal sleep period. Perturbation effect also assessed during their active period to control for its effect during sleep deprivation. Results suggest processes altered during prolonged wakefulness and during sleep.

Organism:

Drosophila melanogaster

Type:

Expression profiling by array, transformed count, 6 protocol, 4 time sets

Platform:

GPL72

Series:

GSE4174

30 Samples

Download data: CEL, EXP

(Submitter supplied) Through an RNAi-based screen, we identified a gene, pdm3, required for sleep maturation in Drosophila. Pdm3, a transcription factor, coordinates an early developmental program that prepares the brain to later execute high levels of juvenile adult sleep. Through temporal mapping, we found that pdm3 acts during the mid-pupal stage of Drosophila development to control sleep ontogeny. As such, we profiled RNA expression in whole brains at this stage of development to identify possible downstream transcriptional changes in the setting of panneuronal pdm3 knockdown (Elav-GAL4> UAS pdm3 RNAi) and controls (Elav-GAL4 / +).

Organism:

Drosophila melanogaster

Type:

Expression profiling by high throughput sequencing

Platform:

GPL21306

8 Samples

Download data: XLSX