Similar studies for GEO DataSets (Select 200057939) - GEO DataSets

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Items: 20

Select item 2000579391.

Gene expression profiling in THP1 cells after PAPD5 or GLD2 knockdown

(Submitter supplied) Next-generation sequencing experiments have shown that microRNAs are expressed in many different isoforms (isomiRs), whose biological relevance is often unclear. We found that mature miR-21, the most widely researched microRNA because of its importance in human disease, is produced in two prevalent isomiR forms that differ by one nucleotide at their 3’ end, and moreover that the 3’ end of miR-21 is post transcriptionally adenylated by the noncanonical poly(A) polymerase PAPD5. more...

Organism:: Homo sapiens

Type:: Expression profiling by array

Platform:: GPL6884
6 Samples

Download data: TXT

Series

Accession:: GSE57939

ID:: 200057939

PubMed Full text in PMC Similar studies Analyze with GEO2R

Select item 2001214662.

Poly(A)-specific ribonuclease sculpts the 3′ ends of microRNAs

(Submitter supplied) The role of 3ʹ-5ʹ exonuclease, PARN, in the 3ʹ end miRNA transcriptome in human cells

Organism:: Homo sapiens

Type:: Non-coding RNA profiling by high throughput sequencing

Platform:: GPL16791
3 Samples

Download data: TXT

Series

Accession:: GSE121466

ID:: 200121466

PubMed Full text in PMC Similar studies SRA Run Selector

Select item 2001395673.

AGO-bound Mature miRNAs with an Exposed 3’ end are Oligo-uridylated by TUTs and Subsequently Degraded by DIS3L2

(Submitter supplied) microRNAs (miRNAs) associating with Argonaute proteins (AGOs) regulate gene expression in mammals. miRNA 3' ends are subject to frequent sequence modifications, which have been proposed to affect miRNA stability. However, the underlying mechanism is not well understood. Here, by genetic and biochemical studies as well as deep sequencing analyses, we find that AGO mutations disrupting miRNA 3' binding are sufficient to trigger extensive miRNA 3’ modifications in HEK293T cells and in cancer patients. more...

Organism:: Homo sapiens

Type:: Non-coding RNA profiling by high throughput sequencing

Platform:: GPL15520
25 Samples

Download data: TSV

Series

Accession:: GSE139567

ID:: 200139567

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Select item 2001213274.

3’ Uridylation Expands miRNA Target Repertoire

(Submitter supplied) Many microRNAs (miRNAs) exist alongside abundant miRNA isoforms (isomiRs), most of which arise from post-maturation sequence modifications, such as 3’ uridylation. However, the ways in which these sequence modifications affect miRNA function remain poorly understood. Here, using human miR-27a in cell lines as a model, we have discovered that a nonfunctional target site unable to base pair extensively with the miRNA seed sequence can regain function when an upstream adenosine is able to base-pair with a post-transcriptionally added uridine in the miR-27a tail. more...

Organism:: Homo sapiens

Type:: Non-coding RNA profiling by high throughput sequencing; Expression profiling by high throughput sequencing

Platforms:: GPL15520 GPL11154
29 Samples

Download data: TAB, TSV

Series

Accession:: GSE121327

ID:: 200121327

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Select item 2000510365.

PARN mediates 3'-end trimming of Argonaute-cleaved precursor microRNAs

(Submitter supplied) microRNAs (miRNAs) are typically generated as ~22-nucleotide double-stranded RNAs via processing of precursor hairpins by the RNase III enzyme Dicer, after which they are loaded into Argonaute (Ago) proteins to form RNA-induced silencing complex (RISC). However, the biogenesis of miR-451, an erythropoietic miRNA conserved in vertebrates, does not require Dicer processing. Instead, the short pre-miR-451 precursor hairpin is directly loaded into Ago, followed by cleavage of the 3' arm and trimming of the 3' end to the mature length by PARN. more...

Organism:: Danio rerio

Type:: Expression profiling by high throughput sequencing

Platform:: GPL14875
5 Samples

Download data: CSV

Series

Accession:: GSE51036

ID:: 200051036

PubMed Full text in PMC Similar studies SRA Run Selector

Select item 2000310376.

Small RNA sequencing in normal and psoriatic skin

(Submitter supplied) We report the application of Illumina small RNA sequencing to normal human skin, as well as uninvolved and involved psoriatic skin. By obtaining over 600 million qualified reads from 20 healthy controls and 47 psoriasis biopsies (uninvolved/involved), we have generated a complete small RNA profile in normal and diseased human skin, with particular emphasis on miRNAs. We report the discovery of 284 putative novel miRNAs as well as 98 differentially expressed miRNAs in psoriatic skin.

Organism:: Homo sapiens

Type:: Non-coding RNA profiling by high throughput sequencing

Platform:: GPL10999
67 Samples

Download data: TXT

Series

Accession:: GSE31037

ID:: 200031037

PubMed Full text in PMC Similar studies SRA Run Selector

Select item 2000310917.

Regulation of miRNA biogenesis by MCPIP1

(Submitter supplied) Effect of MCPIP1 knockdown on miRNA expression profile.

Organism:: Mus musculus; Rattus norvegicus; Murid betaherpesvirus 1; JC polyomavirus; Human immunodeficiency virus 1; Human gammaherpesvirus 8; human gammaherpesvirus 4; Betapolyomavirus macacae; Homo sapiens; Human alphaherpesvirus 1; Human betaherpesvirus 5; Betapolyomavirus hominis; Murid gammaherpesvirus 4

Type:: Non-coding RNA profiling by array

Platform:: GPL7723
2 Samples

Download data: TXT

Series

Accession:: GSE31091

ID:: 200031091

Select item 2000299048.

Post-transcriptional generation of miRNA variants by multiple nucleotidyl transferases contributes to miRNA transcriptome complexity (normal prostate and prostate cells)

(Submitter supplied) Modification of microRNA sequences by the 3' addition of nucleotides to generate so-called isomiR adds to the complexity of miRNA function, with recent reports showing that 3' modifications can influence miRNA stability and efficiency of target repression. Here we show that the 3' modification of miRNAs is a physiological and common post-transcriptional event that shows selectivity for specific miRNAs and is observed across species ranging from C. more...

Organism:: Homo sapiens

Type:: Non-coding RNA profiling by high throughput sequencing

Platforms:: GPL9052 GPL10400
3 Samples

Download data: FA

Series

Accession:: GSE29904

ID:: 200029904

PubMed Full text in PMC Similar studies

Select item 2000269709.

Post-transcriptional generation of miRNA variants by multiple nucleotidyl transferases contributes to miRNA transcriptome complexity

(Submitter supplied) This SuperSeries is composed of the SubSeries listed below.

Organism:: synthetic construct; Homo sapiens

Type:: Other; Non-coding RNA profiling by high throughput sequencing

5 related Platforms
107 Samples

Download data: FA, RCC

Series

Accession:: GSE26970

ID:: 200026970

PubMed Full text in PMC Similar studies Analyze with GEO2R

Select item 20002691910.

Post-transcriptional generation of miRNA variants by multiple nucleotidyl transferases contributes to miRNA transcriptome complexity (Validation 2)

(Submitter supplied) Modification of microRNA sequences by the 3' addition of nucleotides to generate so-called “isomiRs” adds to the complexity of miRNA function, with recent reports showing that 3' modifications can influence miRNA stability and efficiency of target repression. Here we show that the 3' modification of miRNAs is a physiological and common post-transcriptional event that shows selectivity for specific miRNAs and is observed across species ranging from C. more...

Organism:: Homo sapiens; synthetic construct

Type:: Other

Platform:: GPL11669
2 Samples

Download data: TXT

Series

Accession:: GSE26919

ID:: 200026919

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Select item 20002691611.

Post-transcriptional generation of miRNA variants by multiple nucleotidyl transferases contributes to miRNA transcriptome complexity (Validation 1)

Organism:: Homo sapiens; synthetic construct

Type:: Other

Platform:: GPL11668
12 Samples

Download data: TXT

Series

Accession:: GSE26916

ID:: 200026916

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Select item 20002680312.

Post-transcriptional generation of miRNA variants by multiple nucleotidyl transferases contributes to miRNA transcriptome complexity (KD)

Organism:: Homo sapiens

Type:: Other

Platform:: GPL11642
60 Samples

Download data: RCC, TXT

Series

Accession:: GSE26803

ID:: 200026803

PubMed Full text in PMC Similar studies Analyze with GEO2R

Select item 20002680213.

Post-transcriptional generation of miRNA variants by multiple nucleotidyl transferases contributes to miRNA transcriptome complexity (hESC)

Organism:: Homo sapiens

Type:: Other

Platform:: GPL11642
30 Samples

Download data: RCC, TXT

Series

Accession:: GSE26802

ID:: 200026802

PubMed Full text in PMC Similar studies Analyze with GEO2R

Select item 20009365314.

Genome-wide mapping of DROSHA cleavage sites on primary microRNAs and novel substrates

(Submitter supplied) This SuperSeries is composed of the SubSeries listed below.

Organism:: Homo sapiens

Type:: Expression profiling by high throughput sequencing; Other

Platform:: GPL16791
13 Samples

Download data

Series

Accession:: GSE93653

ID:: 200093653

PubMed Similar studies

Select item 20009365115.

Genome-wide mapping of DROSHA cleavage sites on primary microRNAs and novel substrates [fCLIP-seq]

(Submitter supplied) MicroRNA (miRNA) maturation is initiated by DROSHA, a double-stranded RNA (dsRNA)-specific RNase III enzyme. By cleaving primary miRNAs (pri-miRNAs) at specific positions, DROSHA serves as a main determinant of miRNA sequences and a highly selective gate-keeper for the canonical miRNA pathway. However, the sites of DROSHA-mediated processing have not been annotated on a genomic scale, and it remains unclear to what extent DROSHA functions outside the miRNA pathway. more...

Organism:: Homo sapiens

Type:: Other

Platform:: GPL16791
4 Samples

Download data: TSV

Series

Accession:: GSE93651

ID:: 200093651

PubMed Similar studies SRA Run Selector

Select item 20009365016.

Genome-wide mapping of DROSHA cleavage sites on primary microRNAs and novel substrates [RNA-seq]

Organism:: Homo sapiens

Type:: Expression profiling by high throughput sequencing

Platform:: GPL16791
9 Samples

Download data: TSV

Series

Accession:: GSE93650

ID:: 200093650

Select item 20008150717.

Post-transcriptional manipulation of TERC reverses molecular hallmarks of telomere disease

(Submitter supplied) The telomerase RNA component (TERC) is a critical determinant of cellular self renewal. Poly(A)-specific ribonuclease (PARN) is required for post-transcriptional maturation of TERC. PARN mutations lead to incomplete 3′ end processing and increased destruction of nascent TERC RNA transcripts, resulting in telomerase deficiency and telomere diseases. Here, we determined that overexpression of TERC increased telomere length in PARN-deficient cells and hypothesized that decreasing post-transcriptional 3′ oligo-adenylation of TERC would counteract the deleterious effects of PARN mutations. more...

Organism:: Homo sapiens

Type:: Expression profiling by high throughput sequencing

Platform:: GPL16791
8 Samples

Download data: CSV

Series

Accession:: GSE81507

ID:: 200081507

PubMed Full text in PMC Similar studies Analyze with GEO2R SRA Run Selector

Select item 20004143618.

High-throughput sequencing of small RNA transcriptomes and DNA microarrays reveal critical biological features targeted by microRNAs in cell models used for squamous cell cancer research

(Submitter supplied) Background The implication of post-transcriptional regulation by microRNAs in molecular mechanisms underlying cancer disease is well documented. However, their interference at the cellular level is not fully explored. Functional in vitro studies are fundamental for the comprehension of their role; nevertheless results are highly dependable on the adopted cellular model. Next generation small RNA transcriptomic sequencing data of a tumor cell line and keratinocytes derived from primary culture was generated in order to characterize the microRNA content of these systems, thus helping in their understanding. more...

Organism:: Homo sapiens

Type:: Expression profiling by array

Platform:: GPL4133
4 Samples

Download data: TXT

Series

Accession:: GSE41436

ID:: 200041436

PubMed Full text in PMC Similar studies Analyze with GEO2R

Select item 20006304619.

Next generation sequencing reveals microRNA isoforms in liver cirrhosis and hepatocellular carcinoma

(Submitter supplied) Hepatocellular carcinoma (HCC) represents the major histological subtype of liver cancer. Tumorigenic changes in hepatic cells potentially result from aberrant expression of microRNAs (miRNAs). Individual microRNA gene may give rise to miRNAs of different length, named isomiRNAs that proved to be functionally relevant. Results: We detected 374 microRNAs expressed in liver, including miR-122-5p that constituted over 39% ofthe hepatic miRnome. more...