GEO DataSets

(Submitter supplied) Translational control is a key regulatory step in the expression of genes as proteins. In plant cells, translational efficiency of mRNAs differs on different mRNA species, and the efficiency dynamically changes in various conditions. To gain a global view of translational control throughout growth and development, we performed genome-wide analysis of polysome association of mRNA over growth and leaf development in Arabidopsis thaliana by applying the mRNAs in polysome to DNA microarray. more...

Organism:

Arabidopsis thaliana

Type:

Expression profiling by array

Platform:

GPL9020

8 Samples

Download data: TXT

(Submitter supplied) This SuperSeries is composed of the SubSeries listed below.

Organism:

Arabidopsis thaliana

Type:

Expression profiling by array

Platform:

GPL9020

16 Samples

Download data: TXT

(Submitter supplied) Translational control is a key regulatory step in the expression of genes as proteins. In plant cells, translational efficiency of mRNAs differs on different mRNA species, and the efficiency dynamically changes in various conditions. To gain a global view of translational control throughout growth and development, we performed genome-wide analysis of polysome association of mRNA over growth and leaf development in Arabidopsis thaliana by applying the mRNAs in polysome to DNA microarray. more...

Organism:

Arabidopsis thaliana

Type:

Expression profiling by array

Platform:

GPL9020

8 Samples

Download data: TXT

(Submitter supplied) Microarray experiment with polysomal and non-polysomal RNAs extracted under non-stress and mild-dehydration stress. Keywords = translation Keywords = ribosome loading Keywords = polysome Keywords = dehyrdation Keywords: other

Organism:

Arabidopsis thaliana

Type:

Expression profiling by array

Dataset:

GDS1382

Platform:

GPL198

4 Samples

Download data: CEL, EXP

Evaluation of proportion of individual mRNAs in polysomal complexes in leaves under control and mild dehydration stress (DS) conditions. DS reduces level of mRNA in polysomes (ribosome loading) for most genes. Results suggest transcript sequence features that regulate differential mRNA translation.

Organism:

Arabidopsis thaliana

Type:

Expression profiling by array, count, 2 protocol, 2 stress sets

Platform:

GPL198

Series:

GSE2268

4 Samples

Download data: CEL, EXP

(Submitter supplied) 7d-old WT ler seedlings were submitted to 12h of non-stress (air) or hypoxia-stress treatment under low light conditions (45 uM m-2 s-2), and Total and Large Polysome RNA from both treatments were extracted and hybridized against Affymetrix genome chips. Values were used to evaluate changes in transcript abundance and transcript association with large polysomal complexes. TABLE 1 - Comparison between transcript abundance in non-stress and hypoxia-stress conditions. more...

Organism:

Arabidopsis thaliana

Type:

Expression profiling by array

Dataset:

GDS1450

Platform:

GPL198

4 Samples

Download data: CEL, EXP

Expression profiling of 7 day old wild type Ler seedlings subjected to 12 hours of hypoxia under low light conditions. Total RNA and mRNA extracted from large polyribosomes compared. Results provide insight into the role of translational regulation in the response to hypoxia.

Organism:

Arabidopsis thaliana

Type:

Expression profiling by array, count, 2 protocol, 2 stress sets

Platform:

GPL198

Series:

GSE2218

4 Samples

Download data: CEL, EXP

(Submitter supplied) Gene expression analysis of 7d-old Arabidopsis seedlings exposed to short term (2 h) hypoxia, long term (9 h) hypoxia, and 1 h reoxygenation after long term (9 h) hypoxia to evaluate the regulation of gene expression at the level of translation. Keywords: Time Course, hypoxia recovery, polysomal mRNA, IP RNA, polysomes, hypoxia stress, reoxygenation, translational control.

Organism:

Arabidopsis thaliana

Type:

Expression profiling by array

Platform:

GPL198

30 Samples

Download data: CEL

(Submitter supplied) Transcriptome, translatome, and CSP1 RNA regulon analysis of 25-d-o Arabidopsis rosettes exposed to 12h low temperature (4C) treatment.

Organism:

Arabidopsis thaliana

Type:

Expression profiling by array

Platform:

GPL198

38 Samples

Download data: CEL

(Submitter supplied) cea03-02_translatome-cd - translatome-cd - Is there a change in the translation in Cadmium stress condition ? - Comparison between translated RNA (polysomes) and total RNA or non translated RNA (monosomes) in cadmium stress conditions. Keywords: transcribed vs translated,treated vs untreated comparison

Organism:

Arabidopsis thaliana

Type:

Expression profiling by array

Platforms:

GPL4346 GPL5337

20 Samples

Download data: GPR

(Submitter supplied) We analysed the translatome and transcriptome of Arabidopsis thaliana Col-0 WT at five distinct physiological states during seed germination. The aim was to obtain a global overview of genes under translational control during seed-seedling transition.

Organism:

Arabidopsis thaliana

Type:

Expression profiling by array

Platform:

GPL17134

30 Samples

Download data: CEL

(Submitter supplied) Background Protein synthesis is a highly energy demanding process and is regulated according to energy availability in plant cells. Light and sugar availability affect mRNA translation but the specific roles of these factors remain unclear. In this study, sucrose was applied to Arabidopsis seedlings kept in the light or in the dark, in order to distinguish sucrose and light effects on transcription and translation. more...

Organism:

Arabidopsis thaliana

Type:

Expression profiling by array

Platform:

GPL18934

24 Samples

Download data: CEL

(Submitter supplied) Post-transcriptional gene regulation plays a significant role in the response to oxygen deprivation. Here, we utilized advances in next-generation sequencing technology to examine changes in transcriptional control, mRNA loading on to polysome, and regulation of ribosome activity during mRNA translation in 7-day-old Arabidopsis seedlings subjected to 2 hour hypoxia treatment.

Organism:

Arabidopsis thaliana

Type:

Expression profiling by high throughput sequencing

Platform:

GPL13222

14 Samples

Download data: TXT

(Submitter supplied) Microarray comparisons of transcript level in wild-type Arabidopsis and eif3h mutant plants. Goal: To detect any change in transcript level between WT and eif3h mutant. BACKGROUND: The eukaryotic translation initiation factor eIF3 has multiple roles during the initiation of translation of cytoplasmic mRNAs. However, the contributions of individual subunits of eIF3 to the translation of specific mRNAs remain poorly understood. more...

Organism:

Arabidopsis thaliana

Type:

Expression profiling by array

Platform:

GPL198

4 Samples

Download data: CEL, EXP

(Submitter supplied) Microarray comparisons of polysome loading in wild-type Arabidopsis and eif3h mutant Goal: To find the target mRNAs that are translationally regulated by eIF3h. BACKGROUND: The eukaryotic translation initiation factor eIF3 has multiple roles during the initiation of translation of cytoplasmic mRNAs. However, the contributions of individual subunits of eIF3 to the translation of specific mRNAs remain poorly understood. more...

Organism:

Arabidopsis thaliana

Type:

Expression profiling by array

Platform:

GPL198

8 Samples

Download data: CEL, EXP

(Submitter supplied) Heat stress is one of the most prominent and deleterious environmental threads affecting plant growth and development. Upon high temperatures, plants launch specialized gene expression programs that promote stress protection and survival. These programs involve global and specific changes at the transcriptional and translational levels. However the coordination of these processes and their specific role in the establishment of the heat stress response is not fully elucidated. more...

Organism:

Arabidopsis thaliana

Type:

Expression profiling by array; Other

Platform:

GPL198

8 Samples

Download data: CEL

(Submitter supplied) au13-12_polysome - transcriptome and translatome of arabidopsis wt seeds according to dormancy - Identification of transcripts that are differentially abundant (transcriptome) and transcripts that are addressed to translation (translatome) in imbibed Arabidopsis seeds in relation with dormancy. During imbibition of seeds (16h and 24h in darkness at 25°C, dormant and non-dormant seeds), transcriptome analysis is done on total RNA and translatome analysis on polysomal RNA. more...

Organism:

Arabidopsis thaliana

Type:

Expression profiling by array

Platform:

GPL15719

24 Samples

Download data: PAIR

(Submitter supplied) The composition of the transcriptome is regulated by both mRNA synthesis and degradation. One route for mRNA decay is through 5’ decapping, which can be initiated by decapping enzymes and small RNAs. Although decapped RNAs are an important intermediate for mRNA decay, their identity and abundance have never been studied on a large scale. Here we present an experimental method for transcriptome-wide profiling of decapped mRNAs. more...

Organism:

Arabidopsis thaliana

Type:

Expression profiling by array

Platform:

GPL5762

24 Samples

Download data: GPR

(Submitter supplied) Total and polyribosomal RNA was extracted from rosette leaves of 5-6-week-old wild-type and transgenic Arabidopsis plants (ecotype Col-0). After linear amplification using a modified Eberwine procedure, Cy3- or Cy5-labeled cDNA targets were produced from sense-strand RNA through reverse transcription Keywords: repeat sample

Organism:

Arabidopsis thaliana

Type:

Expression profiling by array

Platform:

GPL1787

4 Samples

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(Submitter supplied) This oligonucleotide array is produced from the Operon Version 3.0 Array-Ready Oligo set (AROS), which comprises 29,110 70-mer oligonucleotides representing 26,173 protein-coding genes, and 28,964 protein-encoding gene transcripts, and 87 microRNA gene precursors. AROS design is based on ATH1 release 5.0 of the TIGR Arabidopsis genome annotation database, and Release 4.0 of the miRNA Registry at the Sanger Institute. more...

Organism:

Arabidopsis thaliana

1 Series

4 Samples

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