Similar studies for GEO DataSets (Select 200081826) - GEO DataSets

Select item 2000818261.

Inheritable Silencing of Endogenous Genes by Hit-and-Run Targeted Epigenetic Editing

(Submitter supplied) Gene silencing is a powerful approach for interrogating gene function and holds promise for therapeutic applications. Here we repurpose the ERV silencing machinery of embryonic stem cells to stably silence genes in somatic cells by epigenetics. This was achieved by transiently expressing combinations of artificial transcriptional repressors that bind to and synergize at the target locus to instruct de novo DNA methylation, thus ensuing long-term memory of the repressive epigenetic state. more...

Organism:: Homo sapiens

Type:: Expression profiling by high throughput sequencing; Methylation profiling by high throughput sequencing

Platform:: GPL16791
18 Samples

Download data: BROADPEAK, TXT

Series

Accession:: GSE81826

ID:: 200081826

PubMed Full text in PMC Similar studies Analyze with GEO2R SRA Run Selector

Select item 2001238852.

Engineering epigenetic memory requires co-targeting of histone methylatransferases and DNA methylatransferases

(Submitter supplied) This SuperSeries is composed of the SubSeries listed below.

Organism:: Homo sapiens

Type:: Methylation profiling by array; Genome binding/occupancy profiling by high throughput sequencing

Platforms:: GPL20301 GPL21145
15 Samples

Download data: IDAT

Series

Accession:: GSE123885

ID:: 200123885

PubMed Full text in PMC Similar studies Analyze with GEO2R

Select item 2001238823.

Engineering epigenetic memory requires co-targeting of histone methylatransferases and DNA methylatransferases [ChIP-seq]

(Submitter supplied) Rewriting of the epigenome has risen as a promising alternative to gene editing for precision medicine. In nature, epigenetic silencing can result in complete attenuation of target gene expression over multiple mitotic divisions. However, persistent repression has been difficult to achieve using targeted systems. Here, we report that robust and persistent epigenetic memory required both a DNA methyltransferase (DNMT3A-dCas9) and a histone methyltransferase (Ezh2-dCas9 or KRAB-dCas9). more...

Organism:: Homo sapiens

Type:: Genome binding/occupancy profiling by high throughput sequencing

Platform:: GPL20301
7 Samples

Download data: BEDGRAPH, NARROWPEAK

Series

Accession:: GSE123882

ID:: 200123882

PubMed Full text in PMC Similar studies SRA Run Selector

Select item 2001238304.

Engineering epigenetic memory requires co-targeting of histone methylatransferases and DNA methylatransferases [Methylation]

(Submitter supplied) Rewriting of the epigenome has risen as a promising alternative to gene editing for precision medicine. In nature, epigenetic silencing can result in complete attenuation of target gene expression over multiple mitotic divisions. However, persistent repression has been difficult to achieve using targeted systems. Here, we report that robust and persistent epigenetic memory required both a DNA methyltransferase (DNMT3A-dCas9) and a histone methyltransferase (Ezh2-dCas9 or KRAB-dCas9). more...

Organism:: Homo sapiens

Type:: Methylation profiling by array

Platform:: GPL21145
8 Samples

Download data: IDAT, TXT

Series

Accession:: GSE123830

ID:: 200123830

PubMed Full text in PMC Similar studies Analyze with GEO2R

Select item 2001219985.

Epigenome editing strategies for the functional annotation of topological insulators

(Submitter supplied) The human genome is folded into regulatory units termed ‘topologically-associated domains’ (TADs), within which the majority of gene-enhancer interactions occur. Genome-wide studies support a global role for the insulator protein CTCF in mediating chromosomal looping and the topological constraint of TAD boundaries3,4. However, the impact of individual insulators on enhancer-gene interactions and transcription remains poorly understood. more...

Organism:: Homo sapiens

Type:: Genome binding/occupancy profiling by high throughput sequencing; Methylation profiling by high throughput sequencing

Platform:: GPL18573
74 Samples

Download data: BED, BEDGRAPH, TDF

Series

Accession:: GSE121998

ID:: 200121998

PubMed Full text in PMC Similar studies SRA Run Selector

Select item 2000583236.

Evollutionally dynamic L1 regulation in embryonic stem cells

(Submitter supplied) Mobile elements are important evolutionary forces that challenge genomic integrity. Long interspersed element-1 (L1, also known as LINE-1) is the only autonomous transposon still active in the human genome. It displays an unusual pattern of evolution, with at any given time a single active L1 lineage amplifying to thousands of copies before getting replaced by a new lineage likely under pressure of host restriction factors, which act notably by silencing L1 expression during early embryogenesis. more...

Organism:: Mus musculus; Homo sapiens

Type:: Genome binding/occupancy profiling by high throughput sequencing; Expression profiling by high throughput sequencing

Platforms:: GPL13112 GPL11154
10 Samples

Download data: BED, TXT

Series

Accession:: GSE58323

ID:: 200058323

PubMed Full text in PMC Similar studies SRA Run Selector

Select item 2002438987.

Durable and efficient gene silencing in vivo by hit-and-run epigenome editing [Bisulfite-seq]

(Submitter supplied) Permanent epigenetic silencing with programmable editors equipped with transcriptional repressors holds great promise for the treatment of human diseases. However, to unlock its full therapeutic potential, a formal proof of durable epigenetic silencing upon transient editors’ delivery in vivo is needed. To this end, we targeted here Pcsk9, a hepatocyte-expressed gene involved in cholesterol homeostasis. more...

Organism:: Mus musculus

Type:: Methylation profiling by high throughput sequencing

Platform:: GPL16417
24 Samples

Download data: COV

Series

Accession:: GSE243898

ID:: 200243898

PubMed Full text in PMC Similar studies

Select item 2002409398.

Durable and efficient gene silencing in vivo by hit-and-run epigenome editing [RNAseq_2]

(Submitter supplied) Permanent epigenetic silencing with programmable editors equipped with transcriptional repressors holds great promise for the treatment of human diseases. However, to unlock its full therapeutic potential, a formal proof of durable epigenetic silencing upon transient editors’ delivery in vivo is needed. To this end, we targeted here Pcsk9, a hepatocyte-expressed gene involved in cholesterol homeostasis. more...

Organism:: Mus musculus

Type:: Expression profiling by high throughput sequencing

Platform:: GPL24247
10 Samples

Download data: TXT

Series

Accession:: GSE240939

ID:: 200240939

PubMed Full text in PMC Similar studies

Select item 2002409389.

Durable and efficient gene silencing in vivo by hit-and-run epigenome editing [WGMS_invitro_revision]

(Submitter supplied) Permanent epigenetic silencing with programmable editors equipped with transcriptional repressors holds great promise for the treatment of human diseases. However, to unlock its full therapeutic potential, a formal proof of durable epigenetic silencing upon transient editors’ delivery in vivo is needed. To this end, we targeted here Pcsk9, a hepatocyte-expressed gene involved in cholesterol homeostasis. more...

Organism:: Mus musculus

Type:: Methylation profiling by high throughput sequencing

Platform:: GPL21103
2 Samples

Download data: COV

Series

Accession:: GSE240938

ID:: 200240938

PubMed Full text in PMC Similar studies

Select item 20024093710.

Durable and efficient gene silencing in vivo by hit-and-run epigenome editing [deep_sequencing_ZFPoff8]

(Submitter supplied) Permanent epigenetic silencing with programmable editors equipped with transcriptional repressors holds great promise for the treatment of human diseases. However, to unlock its full therapeutic potential, a formal proof of durable epigenetic silencing upon transient editors’ delivery in vivo is needed. To this end, we targeted here Pcsk9, a hepatocyte-expressed gene involved in cholesterol homeostasis. more...

Organism:: Mus musculus

Type:: Other

Platform:: GPL16417
6 Samples

Download data: TXT

Series

Accession:: GSE240937

ID:: 200240937

PubMed Full text in PMC Similar studies

Select item 20024093611.

Durable and efficient gene silencing in vivo by hit-and-run epigenome editing [deep_sequencing_Cas9]

(Submitter supplied) Permanent epigenetic silencing with programmable editors equipped with transcriptional repressors holds great promise for the treatment of human diseases. However, to unlock its full therapeutic potential, a formal proof of durable epigenetic silencing upon transient editors’ delivery in vivo is needed. To this end, we targeted here Pcsk9, a hepatocyte-expressed gene involved in cholesterol homeostasis. more...

Organism:: Mus musculus

Type:: Other

Platform:: GPL16417
6 Samples

Download data: TXT

Series

Accession:: GSE240936

ID:: 200240936

PubMed Full text in PMC Similar studies

Select item 20024093512.

Durable and efficient gene silencing in vivo by hit-and-run epigenome editing [targetBSseq_offTargets]

(Submitter supplied) Permanent epigenetic silencing with programmable editors equipped with transcriptional repressors holds great promise for the treatment of human diseases. However, to unlock its full therapeutic potential, a formal proof of durable epigenetic silencing upon transient editors’ delivery in vivo is needed. To this end, we targeted here Pcsk9, a hepatocyte-expressed gene involved in cholesterol homeostasis. more...

Organism:: Mus musculus

Type:: Methylation profiling by high throughput sequencing

Platform:: GPL16417
6 Samples

Download data: COV

Series

Accession:: GSE240935

ID:: 200240935

PubMed Full text in PMC Similar studies

Select item 20024093413.

Durable and efficient gene silencing in vivo by hit-and-run epigenome editing [targetBSseq_DMRs_2]

(Submitter supplied) Permanent epigenetic silencing with programmable editors equipped with transcriptional repressors holds great promise for the treatment of human diseases. However, to unlock its full therapeutic potential, a formal proof of durable epigenetic silencing upon transient editors’ delivery in vivo is needed. To this end, we targeted here Pcsk9, a hepatocyte-expressed gene involved in cholesterol homeostasis. more...

Organism:: Mus musculus

Type:: Methylation profiling by high throughput sequencing

Platform:: GPL16417
6 Samples

Download data: COV

Series

Accession:: GSE240934

ID:: 200240934

PubMed Full text in PMC Similar studies

Select item 20024093314.

Durable and efficient gene silencing in vivo by hit-and-run epigenome editing [targetBSseq_DMRs]

(Submitter supplied) Permanent epigenetic silencing with programmable editors equipped with transcriptional repressors holds great promise for the treatment of human diseases. However, to unlock its full therapeutic potential, a formal proof of durable epigenetic silencing upon transient editors’ delivery in vivo is needed. To this end, we targeted here Pcsk9, a hepatocyte-expressed gene involved in cholesterol homeostasis. more...

Organism:: Mus musculus

Type:: Methylation profiling by high throughput sequencing

Platform:: GPL16417
6 Samples

Download data: COV

Series

Accession:: GSE240933

ID:: 200240933

PubMed Full text in PMC Similar studies

Select item 20024093215.

Durable and efficient gene silencing in vivo by hit-and-run epigenome editing [targetBSseq_CpG_Pcsk9_2]

(Submitter supplied) Permanent epigenetic silencing with programmable editors equipped with transcriptional repressors holds great promise for the treatment of human diseases. However, to unlock its full therapeutic potential, a formal proof of durable epigenetic silencing upon transient editors’ delivery in vivo is needed. To this end, we targeted here Pcsk9, a hepatocyte-expressed gene involved in cholesterol homeostasis. more...

Organism:: Mus musculus

Type:: Methylation profiling by high throughput sequencing

Platform:: GPL16417
9 Samples

Download data: COV

Series

Accession:: GSE240932

ID:: 200240932

PubMed Full text in PMC Similar studies

Select item 20022620916.

Durable and efficient gene silencing in vivo by hit-and-run epigenome editing

(Submitter supplied) This SuperSeries is composed of the SubSeries listed below.

Organism:: Mus musculus

Type:: Expression profiling by high throughput sequencing; Methylation profiling by high throughput sequencing; Other

Platforms:: GPL16417 GPL21103 GPL24247
121 Samples

Download data: COV, TXT

Series

Accession:: GSE226209

ID:: 200226209

PubMed Full text in PMC Similar studies

Select item 20022620017.

Durable and efficient gene silencing in vivo by hit-and-run epigenome editing [WGMS]

(Submitter supplied) Permanent epigenetic silencing with programmable editors equipped with transcriptional repressors holds great promise for the treatment of human diseases. However, to unlock its full therapeutic potential, a formal proof of durable epigenetic silencing upon transient editors’ delivery in vivo is needed. To this end, we targeted here Pcsk9, a hepatocyte-expressed gene involved in cholesterol homeostasis. more...

Organism:: Mus musculus

Type:: Methylation profiling by high throughput sequencing

Platform:: GPL21103
15 Samples

Download data: COV

Series

Accession:: GSE226200

ID:: 200226200

PubMed Full text in PMC Similar studies

Select item 20022618818.

Durable and efficient gene silencing in vivo by hit-and-run epigenome editing [targeBSseq]

(Submitter supplied) Permanent epigenetic silencing with programmable editors equipped with transcriptional repressors holds great promise for the treatment of human diseases. However, to unlock its full therapeutic potential, a formal proof of durable epigenetic silencing upon transient editors’ delivery in vivo is needed. To this end, we targeted here Pcsk9, a hepatocyte-expressed gene involved in cholesterol homeostasis. more...

Organism:: Mus musculus

Type:: Methylation profiling by high throughput sequencing

Platform:: GPL16417
16 Samples

Download data: COV

Series

Accession:: GSE226188

ID:: 200226188

PubMed Full text in PMC Similar studies

Select item 20022617819.

Durable and efficient gene silencing in vivo by hit-and-run epigenome editing [RNA-seq]

(Submitter supplied) Permanent epigenetic silencing with programmable editors equipped with transcriptional repressors holds great promise for the treatment of human diseases. However, to unlock its full therapeutic potential, a formal proof of durable epigenetic silencing upon transient editors’ delivery in vivo is needed. To this end, we targeted here Pcsk9, a hepatocyte-expressed gene involved in cholesterol homeostasis. more...

Organism:: Mus musculus

Type:: Expression profiling by high throughput sequencing

Platform:: GPL24247
15 Samples

Download data: TXT

Series

Accession:: GSE226178

ID:: 200226178

PubMed Full text in PMC Similar studies

Select item 20014218120.

Engineering of effector domains for targeted DNA methylation with reduced off-target effects

(Submitter supplied) Epigenome editing is a promising technology, potentially allowing the stable reprogramming of gene expression profiles without alteration of the DNA sequence. Targeted DNA methylation has been successfully documented by many groups for silencing of selected genes, but recent publications have raised concerns regarding its specificity. In the current work, we developed new EpiEditors for programmable DNA methylation in cells with a high efficiency and improved specificity. more...

Organism:: Homo sapiens

Type:: Methylation profiling by high throughput sequencing

Platform:: GPL21290
4 Samples

Download data: BED, BIGWIG

Series

Accession:: GSE142181

ID:: 200142181

PubMed Full text in PMC Similar studies SRA Run Selector

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