GEO DataSets

(Submitter supplied) Changes in cell adhesion molecule (CAM) expression and miRNAs regulating them are known to be involved in malignant progression in colon cancer. We investigated expression profiles of CAM genes and non-coding RNAs in CaCo2 colon cancer cells in static culture and under dynamic flow conditions perfused in microfluidic chip emulating physiological microenvironment. We incubated monolayers of CaCo2 cells in Transwell® units either under static conditions or under flow in a microfluidic chip. more...

Organism:

Homo sapiens; synthetic construct

Type:

Expression profiling by high throughput sequencing; Non-coding RNA profiling by array

Platforms:

GPL21572 GPL6244

12 Samples

Download data: CEL, CHP

(Submitter supplied) Custom array chip focusing on transporters and ion channels. Genes are represented by 70 mers.

Organism:

Homo sapiens

1 DataSet

1 Series

32 Samples

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(Submitter supplied) Gut-on-chip devices enable exposure of cells to a continuous flow of culture medium, inducing shear stresses and could thus better recapitulate the in vivo human intestinal environment in an in vitro epithelial model compared to static culture methods. We aimed to study if dynamic culture conditions affect the gene expression of Caco-2 cells cultured statically or dynamically in a gut-on-chip device and how these gene expression patterns compared to that of intestinal segments in vivo. more...

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL17692

8 Samples

Download data: CEL

(Submitter supplied) The multifunctional intestinal mucus layer plays a crucial role in human health. Our understanding of the human colonic mucus layer in terms of structure, function and has been largely dependent on expensive and advanced ex vivo or in vitro models, which often require high expertise. The mucus-producing intestinal cell line HT29-MTX-E12 has been commonly used in more simple in vitro models, but produces only low amounts of the intestine-specific MUC2. more...

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL17692

15 Samples

Download data: CEL

(Submitter supplied) Due to the widespread application of food-relevant inorganic nanomaterials, the gastrointestinal tract is potentially exposed to these materials. Gut-on-chip in vitro model systems are proposed for the investigation of compound toxicity as they better recapitulate the in vivo human intestinal environment than static models, due to the added shear stresses associated with the flow of medium in line with what cells experience in vivo. more...

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL17692

31 Samples

Download data: CEL