GEO DataSets

(Submitter supplied) In vivo microarray study of global gene expression changes in peripheral blood mononuclear cells (PBMCs) of Pietrain pigs during the stage of inatte immune and adaptive immune response to porcine reproductive and respiratory syndrome virus vaccination.

Organism:

Sus scrofa

Type:

Expression profiling by array

Platforms:

GPL21514 GPL16493

18 Samples

Download data: CEL

(Submitter supplied) In vivo microarray study of global gene expression changes in peripheral blood mononuclear cells (PBMCs) of German Landrace pigs during the innate immune response to porcine reproductive and respiratory syndrome virus vaccination.

Organism:

Sus scrofa

Type:

Expression profiling by array

Platforms:

GPL21514 GPL16493

42 Samples

Download data: CEL

(Submitter supplied) Porcine Reproductive and Respiratory Syndrome Virus (PRRSV) infection of 3rd trimester pregnant pigs can result in transmission of the virus to the fetus and ultimately death in utero or postnatally. Little is known about the immune response to infection at the maternal-fetal interface and in the fetus itself, or the molecular events behind virus transmission and disease progression in the fetus. To investigate these processes, RNA-sequencing of two tissues, uterine endothelium adjacent to the umbilical attachment site and fetal thymus, was performed 21 days post challenge on four groups of fetuses selected from a large PRRSV challenge experiment of pregnant gilts.

Organism:

Sus scrofa

Type:

Expression profiling by high throughput sequencing

Platform:

GPL11429

96 Samples

Download data: TXT

(Submitter supplied) Purpose:We performed next-generation RNA sequencing and a comprehensive bioinformatics analyses to characterize the transcriptome landscapes, including mRNAs and long noncoding RNAs (lncRNAs) in PRRSV-infected PAMs or IFN-stimulated PAMs at different time points. Conclusions: We showed that PRRSV infection displays different transcriptome profiles of PAMs, and the aberrant function of PAMs may be reflected by significant down-regulation of essential genes at the transcriptome levels. more...

Organism:

Sus scrofa

Type:

Expression profiling by high throughput sequencing; Non-coding RNA profiling by high throughput sequencing

Platform:

GPL19176

12 Samples

Download data: TXT

(Submitter supplied) Investigation of the transcriptome profile of lung dendritic cells (DCs) of two genetically different pig breeds (Pietrain and Duroc) after PRRSV infection in vitro and determination of the temporal changes in transcriptional profiles.

Organism:

Sus scrofa

Type:

Expression profiling by high throughput sequencing

Platform:

GPL11429

12 Samples

Download data: XLSX

(Submitter supplied) Porcine reproductive and respiratory syndrome virus (PRRSV) is a causative agent of an important infectious disease causing serious economic losses to swine industry called PRRS (porcine reproductive and respiratory syndrome). The clinical signs of this syndrome indcude respiratory disorders, abortions and variable mortality in piglets. To compare the virulence of highly diverse East European strains belonging to subtype 2 (Russian strain ILI and Belarusian strain BOR) and Danish strain from classical subtype 1 (DAN) the experimental study enrolling infection of piglets was performed. more...

Organism:

Sus scrofa

Type:

Expression profiling by array

Platform:

GPL23094

12 Samples

Download data: TXT

(Submitter supplied) Porcine reproductive and respiratory syndrome caused by porcine reproductive and respiratory syndrome virus (PRRSV) is an infectious disease characterized by severe reproductive deficiency in pregnant sows, respiratory symptoms in piglets, and high mortality. In this study, we employed Affymetrix microarray chip technology to compare the gene expression profiles of lung tissue samples from Dapulian (DPL) pigs (a Chinese indigenous pig breed) and Duroc×Landrace×Yorkshire (DLY) pigs after infection with PRRSV. more...

Organism:

Sus scrofa

Type:

Expression profiling by array

Platform:

GPL3533

6 Samples

Download data: CEL, CHP, TXT

(Submitter supplied) BACKGROUND: There was a large scale outbreak of the highly pathogenic porcine reproductive and respiratory syndrome (PRRS) in China and Vietnam during 2006 and 2007 that resulted in unusually high morbidity and mortality among pigs of all ages. The mechanisms underlying the molecular pathogenesis of the highly virulent PRRS virus (H-PRRSV) remains unknown. Therefore, the relationship between pulmonary gene expression profiles after H-PRRSV infection and infection pathology were analyzed in this study using high-throughput deep sequencing and histopathology. more...

Organism:

Sus scrofa

Type:

Expression profiling by high throughput sequencing

Platform:

GPL9126

3 Samples

Download data

(Submitter supplied) The Porcine Reproductive and Respiratory Syndrome (PRRS) has an extensive impact on pig production. The causative virus (PRRSV) is divided in two species, PRRSV-1 (European origin) and PRRSV-2 (North American origin). Within PRRSV-1, PRRSV-1.3 strains such as Lena are more pathogenic than PRRSV-1.1 strains such as Flanders 13 (FL13). To date, the molecular interactions of PRRSV with primary lung mononuclear phagocytes (MNP) subtypes including conventional dendritic cells type 1 (cDC1) and type 2 (cDC2), monocyte-derived DCs (moDC) and parenchymal macrophages (PIM) have not been thoroughly investigated. more...

Organism:

Sus scrofa

Type:

Expression profiling by high throughput sequencing

Platform:

GPL20983

408 Samples

Download data: TSV

(Submitter supplied) Purpose: To characterize the differential microRNA expression profiles and microRNA editing upon PRRSV infection, using NGS techonology, we sequenced small RNAs of the lungs of Tongcheng and Landrace pigs before (0 dpi) and after (3, 5, 7 dpi) infection with high-pathogenic PRRSV (HP-PRRSV). Methods: The miRNA expression profiles of the lungs of Tongcheng and Landrace pigs before (0 dpi) and after (3, 5, 7 dpi) HP-PRRSV infection were produced by using solexa platform. more...

Organism:

Sus scrofa

Type:

Non-coding RNA profiling by high throughput sequencing

Platform:

GPL10945

8 Samples

Download data: TXT

(Submitter supplied) Both virulent and live-attenuated PRRSV strains can establish persistent infection in lymphoid tissues of pigs. To investigate the mechanisms of PRRSV persistence, we performed transcriptional analysis of inguinal lymphoid tissue of pigs experimentally infected with an attenuated PRRSV strain. A total of 6,404 differentially expressed genes (DEGs) were detected of which 3,690 DEGs were upregulated and 2,444 DEGs were downregulated. more...

Organism:

Sus scrofa

Type:

Expression profiling by high throughput sequencing

Platform:

GPL11429

10 Samples

Download data: TSV

(Submitter supplied) Porcine reproductive and respiratory syndrome (PRRS) has been one of the most economically important diseases affecting swine industry worldwide and causes great economic losses each year. PRRS virus (PRRSV) replicates mainly in porcine alveolar macrophages (PAMs) and dendritic cells (DCs) and develops persistent infections, antibody-dependent enhancement (ADE), interstitial pneumonia and immunosuppression. more...

Organism:

Sus scrofa

Type:

Expression profiling by high throughput sequencing

Platform:

GPL9126

3 Samples

Download data

(Submitter supplied) To better understand how innate immunity to vaccination can lead to lasting protective immunity, we have used systemic bioinformatics approaches to define the signature of the yellow fever specific immune response in a cohort of 21 volunteers.

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL21975

109 Samples

Download data: CEL

(Submitter supplied) Using high-throughput technology, we aim to identify the expression of mRNAs for understanding the comparative pathogenesis of CSF in PBMCs and Macrophages of indigenous verses crossbred pigs.

Organism:

Sus scrofa

Type:

Expression profiling by high throughput sequencing

Platform:

GPL20983

6 Samples

Download data: TXT

(Submitter supplied) Systems biology has the potential to identify gene signatures associated with vaccine immunogenicity or protective efficacy. The main objective of our study was to identify optimal post-vaccination time points for evaluating blood RNA-expression profiles in recipients of the candidate tuberculosis vaccine M72/AS01. In this phase II open-label study (NCT01669096), healthy Bacillus Calmette-Guérin (BCG)-primed, HIV-negative adults were administered two doses (30-days apart) of M72/AS01. more...

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL570

164 Samples

Download data: CEL

(Submitter supplied) Three groups of German Landrace piglets were vaccinated with tetanus toxoid. Transcriptome profiles of PBMC were analysed from blood samples taken 0, 2, 4, 8, 24 and 75 hours after a first vaccination and 0, 2, 4, 8, 24 and 75 hours as well as 14 d after a second vaccination on day 14.

Organism:

Sus scrofa

Type:

Expression profiling by array

Platform:

GPL3533

39 Samples

Download data: CEL

(Submitter supplied) In this study, we generated the PBMC transcriptome data of 3 Landrace, and 3 indigenous Ghurrah pigs before and 7 days after Classical Swine fever vaccination. The piglets were 8-12 weeks of age. The sequencing was done on Illumina X-Ten platform, generating average of 39 million 150 bp paired end reads for each sample.

Organism:

Sus scrofa

Type:

Expression profiling by high throughput sequencing

Platform:

GPL22918

12 Samples

Download data: TXT

(Submitter supplied) In vivo microarray study of transcriptional changes in porcine peripheral blood mononuclear cells (PBMCs) during the immune response to tetanus vaccination

Organism:

Sus scrofa

Type:

Expression profiling by array

Platform:

GPL16569

112 Samples

Download data: CEL

(Submitter supplied) Here, we integrated genome-wide genotype, gene expression and phenotype (viremia level (VL) and weight gain (WG)) data to identify genetic variations and genes that are associated with variation in pigs’ response to PRRSV infection. RNA-seq analysis of peripheral blood samples collected just prior to experimental challenge (day 0) and at 4, 7, 10 and 14 days post infection from 44 pigs revealed 6430 differentially expressed genes after infection. more...

Organism:

Sus scrofa

Type:

Expression profiling by high throughput sequencing

Platform:

GPL11429

179 Samples

Download data: TXT

(Submitter supplied) This study was undertaken to obtain a global view of the temporal changes in equine immune gene expression between from the neonatal stage to adulthood. To this end, transcriptome analysis of peripheral blood mononuclear cells (PBMCs) from foals on the day of birth and again 6 weeks later was performed and contrasted with those of adult PBMCs. Differential gene expression analysis identified 2,280 genes with ≥2 fold change between PBMCs sampled on day 1 and 42 (p≤0.01). more...

Organism:

Equus caballus

Type:

Expression profiling by high throughput sequencing

Platform:

GPL21401

12 Samples

Download data: TXT