GEO DataSets

(Submitter supplied) RNA-seq was used to analysis of mazEF toxin-antitoxin system mediated post-transcriptional inhibition through its endoribonuclease activity. DNA damage agents ,such as MMC, activates the mazEF system leading to cells growth arrest. In the present study, results reveal that a larger number of genes involved in amino acid and carbohydrate metabolism, ion transport and transcription was down-regulated in the wild-type compared with the mazEF-dr mutant.

Organism:

Deinococcus radiodurans

Type:

Expression profiling by high throughput sequencing

Platform:

GPL23271

4 Samples

Download data: XLSX

(Submitter supplied) A crucial bacterial strategy to avoid killing by antibiotics is to enter a growth arrested state, yet the molecular mechanisms behind this process remain elusive. The conditional overexpression of MazF, the endoribonuclease toxin of the MazEF toxin-antitoxin system in Staphylococcus aureus, is one approach to induce bacterial growth arrest, but its targets remain largely unknown. We used overexpression of MazF and high-throughput sequence analysis following the exact mapping of non-phosphorylated transcriptome ends (nEMOTE) technique to reveal in vivo toxin cleavage sites on a global scale. more...

Organism:

Staphylococcus aureus

Type:

Expression profiling by high throughput sequencing; Other

Platforms:

GPL19006 GPL25144

10 Samples

Download data: TSV