GEO DataSets

(Submitter supplied) RNAseq and LC/MS metabolomics analysis of C. difficile strain 630 grown in BHIS media with 50% (vol/vol) faecal water added, compared with control BHIS containing only the additional PBS used for prep of Faecal water. Cells grown in biological triplicates to late log phase (T=6h) prior to harvest. Goal was to determine changes in gene expression caused by exposure to Faecal water, and changes in the metabolite profile of faecal water containing medium when incubated with actively growing C. more...

Organism:

Clostridioides difficile 630

Type:

Expression profiling by high throughput sequencing

Platform:

GPL24774

6 Samples

Download data: TXT

(Submitter supplied) Transcriptionnal profiling of C. difficile R20291 strain vs a R20291 strain after4h 6h 8h 14h 38h of growth in Vivo

Organism:

Clostridioides difficile

Type:

Expression profiling by array

Platform:

GPL15218

19 Samples

Download data: GPR, XLS

(Submitter supplied) The incidence of Clostridium difficile infection has been steadily rising over the past decade. Its increased rate is associated with the specific NAP1/BI/027 strains which are “hypervirulent” and have led to several large outbreaks since their emergence. However, the relation between their outbreaks and virulence regulation mechanisms remains unclear. It has been reported that the major virulence factor TcdA and TcdB in C. more...

Organism:

Clostridioides difficile R20291

Type:

Expression profiling by high throughput sequencing

Platform:

GPL24374

12 Samples

Download data: XLSX

(Submitter supplied) Purpose: A goal of this study was to identify genes induced in the mother cell during Clostridium difficile sporulation (specifically in a σE-, σK-, and SpoIIID-dependent manner). Methods: Whole genome RNA sequencing was performed on wildtype, sigE-, sigK- and spoIIID- C. difficile strains (strain 630 background; JIR8094 = parent strain), and the transcriptional profiles of the different mutants during growth on 70:30 agar plates were determined using an Illumina MiSeq1000. more...

Organism:

Clostridioides difficile 630

Type:

Expression profiling by high throughput sequencing

Platform:

GPL19481

12 Samples

Download data: TXT

(Submitter supplied) Clostridium difficile is an important nosocomial pathogen and the leading cause of hospital-acquired diarrhea. Antibiotic use is the primary risk factor for the development of C. difficile-associated disease because it disrupts normal protective gut flora and enables C. difficile to colonize the colon. C. difficile damages host tissue by secreting toxins and disseminates by forming spores. The toxin-encoding genes, tcdA and tcdB are part of a pathogenicity locus, which also encodes the gene tcdR that codes for the toxin genes positive regulator. more...

Organism:

Clostridioides difficile

Type:

Expression profiling by high throughput sequencing

Platform:

GPL22304

4 Samples

Download data: XLS

(Submitter supplied) Genes encoding the alternative sigma factor SigmaB, involved in the general stress response in firmicutes, as well as its regulators are present in the genome of the enteropathogen Clostridium difficile. We inactivated the sigB gene using the ClosTron technology in order to identify the role of this sigma factor in the physiology of this bacterium. Transcriptomic experiments showed that SigB positively and negatively controlls several genes involved in different cellular processes such as metabolism, sporulation and stress response.

Organism:

Clostridioides difficile

Type:

Expression profiling by array

Platform:

GPL18319

4 Samples

Download data: GPR, TXT, XLS

(Submitter supplied) The c-di-GMP regulon in C. difficile

Organism:

Clostridioides difficile

Type:

Expression profiling by high throughput sequencing

Platform:

GPL22510

8 Samples

Download data: XLSX

(Submitter supplied) Investigation of whole genome gene expression level changes in a Clostridium difficile fur (ferric uptake regulator) mutant, compared to the wild type strain 630 erm. The fur mutant analyzed in this study is further described in Ho and Ellermeier (2015) J. Bacteriology

Organism:

Clostridioides difficile 630

Type:

Expression profiling by array

Platform:

GPL20243

12 Samples

Download data: PAIR