GEO DataSets

(Submitter supplied) We sought to determine how CovRS mutations varying CovR phosphorylation levels affect the gene expression profile of group A streptococcus

Organism:

Streptococcus sp. 'group A'

Type:

Expression profiling by high throughput sequencing

Platform:

GPL19691

32 Samples

Download data: TXT

(Submitter supplied) We sought to determine how single amino acid changes in the active site of CovS autophosphorylation and subsequent phosphorylation of the response regulator CovR affect the gene expression profile of group A streptococcus

Organism:

Streptococcus sp. 'group A'

Type:

Expression profiling by high throughput sequencing

Platform:

GPL19691

24 Samples

Download data: TXT

(Submitter supplied) The control of virulence two-component gene regulatory system (CovRS) is critical to the pathogenesis of many medically important streptococci. In emm1 group A streptococci (GAS), CovR directly binds the promoters of numerous GAS virulence factor encoding genes. Elimination of CovS phosphatase activity increases CovR phosphorylation (CovR~P) levels and abrogates GAS virulence. Given the emm type-specific diversity of CovRS function, herein we used ChIP-seq to define global CovR DNA occupancy in the wild-type emm3 strain MGAS10870 (medium CovR~P) and its CovS phosphatase-negative derivative 10870-CovS-T284A (high CovR~P). more...

Organism:

Streptococcus pyogenes

Type:

Genome binding/occupancy profiling by high throughput sequencing

Platform:

GPL28678

11 Samples

Download data: XLSX

(Submitter supplied) We investigated the covS-regulation of S. pyogenes in a hypervirulent M23 strain using RNA-sequencing. The differential gene expression comparison between the covS- mutant and isogenic wild type covS+ identified altered expression of 349 (18%) genes, including a broad spectrum of virulence genes and diverse metabolic genes. The data showed that the strain achieved hypervirulence by enhancing the expression of genes responsible for invasiveness and antiphagocytosis (i.e., hasABC), by abrogating the expression of toxic genes (i.e., speB), and by compromising gene products with dispensable functions (i.e., sfb1). more...

Organism:

Streptococcus pyogenes

Type:

Expression profiling by high throughput sequencing

Platform:

GPL19982

8 Samples

Download data: XLS

(Submitter supplied) Two covR mutant derivatives of parental strain MGAS2221 were recovered from mice experimentally infected with MGAS2221 and shown to differ in terms of the number and concentration of secreted proteins. One of the covR mutant strains had a secretion phenotype identical to a covS mutant strain, while the other had a secretion phenotype identical to a constructed covR mutant strain. To further investigate the potential differences between the two covR mutant strains we performed expression microarray analysis.

Organism:

Coxiella burnetii; Chlamydia trachomatis; Streptococcus pyogenes; Borreliella burgdorferi; Yersinia pestis; Staphylococcus aureus

Type:

Expression profiling by array

Platform:

GPL2129

4 Samples

Download data: CEL

(Submitter supplied) We performed CovR ChIP-seq analysis in the emm1 strain MGAS2221 and its CovS kinase deficient derivative strain 2221-CovS-E281A. We identified that CovR bound in the promoter regions of nearly all virulence factor encoding genes in the CovR regulon. Additionally, direct CovR binding was observed for numerous genes encoding proteins involved in amino acid metabolism, but we found limited direct CovR binding to genes encoding other transcriptional regulators. more...

Organism:

Streptococcus pyogenes

Type:

Genome binding/occupancy profiling by high throughput sequencing

Platform:

GPL31155

10 Samples

Download data: XLSX

(Submitter supplied) To determine the impact of CovS inactivation in acapsular GAS (Streptococcus pyogenes) strains

Organism:

Streptococcus pyogenes

Type:

Expression profiling by high throughput sequencing

Platform:

GPL28678

12 Samples

Download data: CSV

(Submitter supplied) We sequenced mRNA transcripts from three isogenic M3 serotype GAS strains, parental (MGAS10870), complement (10870::rocAM1), and deletion (10870ΔrocA). There were no significant changes between the parental and revertant strain. Comparison of the parental and complemented strain revealed several virulence factors were up-regulated in the parental strain where RocA function was diminished. We concluded that RocA, through direct or indirect mechanisms, is able to control numerous virulence genes and this lack of RocA regulation increases expression of virulence factors, which contributes to the hyper-virulent state of serotype M3 GAS.

Organism:

Streptococcus pyogenes

Type:

Expression profiling by high throughput sequencing

Platform:

GPL19982

3 Samples

Download data: XLSX

(Submitter supplied) We used RNA-Seq to assess how RocA regulates gene expression in stationary phase GAS cultures

Organism:

Streptococcus pyogenes

Type:

Expression profiling by high throughput sequencing

Platform:

GPL18784

4 Samples

Download data: TXT

(Submitter supplied) We used RNA-Seq to assess how RocA regulates gene expression in stationary phase cultures of serotype M1 GAS

Organism:

Streptococcus pyogenes

Type:

Expression profiling by high throughput sequencing

Platform:

GPL18784

4 Samples

Download data: TXT

(Submitter supplied) We used RNA-Seq to assess whether the rocA gene regulates gene expression in M1 GAS

Organism:

Streptococcus pyogenes

Type:

Expression profiling by high throughput sequencing

Platform:

GPL20656

6 Samples

Download data: XLSX

(Submitter supplied) We used RNA-Seq to assess whether the rocA gene regulates gene expression in M1 GAS

Organism:

Streptococcus pyogenes

Type:

Expression profiling by high throughput sequencing

Platform:

GPL19982

3 Samples

Download data: XLS

(Submitter supplied) Microarray was performed on RNA extracted from mid-logarithmic phase in vitro grown non-M1 Streptococcus pyogenes isolates, with either intact or mutant covRS operons. These isolates were compared with corresponding M1 covRS intact and mutant forms to link the expression prolfiles of these non-M1 isolates with invasive pathogenesis.

Organism:

Streptococcus pyogenes

Type:

Expression profiling by array

Platform:

GPL10857

32 Samples

Download data: GPR

(Submitter supplied) Manuscript title: The endopeptidase PepO regulates the SpeB cysteine protease and is essential for the virulence of invasive M1T1 Streptococcus pyogenes. The study investigates the effect of pepO mutation on global gene expression in GAS M1T1 strain 5448.

Organism:

Streptococcus pyogenes

Type:

Expression profiling by array

Platform:

GPL19152

3 Samples

Download data: TXT, XLSX

(Submitter supplied) The goal of this study were to compare the transcriptoms of CcpA and CovR in group A Streptococcus. Isogenic mutant strains were created in which CcpA and CovR were inactivated alone and in combination. The transcriptomes of the four strains (wild-type, CcpA mutant, CovR mutant, and ccpA-covR double mutant) were then compared.

Organism:

Streptococcus pyogenes; Streptococcus sp. 'group A'

Type:

Expression profiling by array

Platform:

GPL9080

32 Samples

Download data: CEL

(Submitter supplied) Group A streptococci (GAS) may engage different sets of virulence strategies, depending on the site of infection and host context. We previously isolated 2 phenotypic variants of a globally disseminated M1T1 GAS clone: a virulent wild-type (WT) strain, characterized by a SpeB(+)/SpeA(-)/Sda1(low) phenotype, and a hypervirulent animal-passaged (AP) strain, better adapted to survive in vivo, with a SpeB(-)/SpeA(+)/Sda1(high) phenotype. more...

Organism:

Streptococcus pyogenes

Type:

Expression profiling by array

Platform:

GPL10950

40 Samples

Download data

(Submitter supplied) We report the mRNA expression profile of Streptococcus pyogeens Type M1 strain M1T15448-derived mutant lacking phospotyrosine phosphatase.

Organism:

Streptococcus pyogenes

Type:

Non-coding RNA profiling by high throughput sequencing

Platform:

GPL18784

4 Samples

Download data: TXT

(Submitter supplied) We sought understand the molecular mehcanism of gene regulation by Mga and its contribution to GAS pathogenesis in serotype M59 GAS through whole transcriptome analysis of strains with phosphorylation mimicking substitutions in key histidine residues of Mga.

Organism:

Streptococcus pyogenes MGAS15252

Type:

Expression profiling by high throughput sequencing

Platform:

GPL20059

18 Samples

Download data: XLSX

(Submitter supplied) The aim of this study was to determine the extent of the trxR regulon in the M1 serotype MGAS5005. Gold, Leday, McIver 2008 Keywords: Wild-type vs trxR-

Organism:

Streptococcus pyogenes

Type:

Expression profiling by array

Platform:

GPL1482

6 Samples

Download data: GPR

(Submitter supplied) This SuperSeries is composed of the SubSeries listed below.

Organism:

Streptococcus pyogenes

Type:

Expression profiling by high throughput sequencing; Genome binding/occupancy profiling by high throughput sequencing

Platforms:

GPL24880 GPL20656

12 Samples

Download data: BED, WIG