GEO DataSets

(Submitter supplied) Due to limitations of available human models for development of gene expression based radiation biodosimetry, many such studies have made use of mouse models. To provide a broad view of the gene expression response to irradiation in the mouse, we have exposed male C57BL/6 mice to 0, 1.5, 3, 6 or 10 Gy of gamma rays, sacrificing groups of the mice at 1, 2, 3, 5, or 7 days after exposure. Global gene expression was then profiled in blood from individual mice using Agilent microarrays. more...

Organism:

Mus musculus

Type:

Expression profiling by array

Platform:

GPL11202

248 Samples

Download data: TXT

(Submitter supplied) Background: In the event of an improvised nuclear device detonation, the prompt radiation exposure would consist of γ rays plus a neutron component that would contribute to the total dose. As neutrons cause more complex and difficult to repair damage to cells that would result in more severe health burden to affected individuals, it is paramount to be able to estimate the contribution of neutrons to an estimated dose, to provide information for those making treatment decisions. more...

Organism:

Mus musculus

Type:

Expression profiling by array

Platform:

GPL10333

72 Samples

Download data: TXT

(Submitter supplied) Background: Radiation exposure due to the detonation of an improvised nuclear device remains a major security concern. Radiation from such a device involves a combination of photons and neutrons. Although photons will make the greater contribution to the total dose, neutrons will certainly have an impact on the severity of the exposure as they have high relative biological effectiveness. Results: We investigated the gene expression signatures in the blood of mice exposed to 3 Gy x-rays, 0.75 Gy of neutrons, or to mixed field photon/neutron with the neutron fraction contributing 5%, 15%, or 25% of a total 3 Gy radiation dose. more...

Organism:

Mus musculus

Type:

Expression profiling by array

Platform:

GPL11202

36 Samples

Download data: TXT

(Submitter supplied) The detonation of an improvised nuclear device would produce prompt radiation consisting of both photons (gamma-rays) and neutrons. While much effort in recent years has gone into the development of radiation biodosimetry methods suitable for mass triage, the possible effect of neutrons on the endpoints studied has remained largely uninvestigated. We have used a novel neutron irradiator with an energy spectrum based on that 1-1.5 km from the epicenter of the Hiroshima blast to begin examining the effect of neutrons on global gene expression, and the impact this may have on the development of gene expression signatures for radiation biodosimetry. more...

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL13497

92 Samples

Download data: TXT

(Submitter supplied) In the current global scenario there is always a possibility that a terrorist group might acquire material to produce a nuclear device. In the likelihood of a detonation of an improvised nuclear device (IND) the prompt radiation would consist of both photons (gamma rays) and neutrons. As neutrons generally have a high Relative Biological Effectiveness (RBE) for most physiological endpoints, it is important to understand the impact that neutrons would have on the biodosimetry methods that are being developed for medical triage purposes. more...

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL13497

30 Samples

Download data: TXT

(Submitter supplied) Cesium-137 is a radionuclide of concern in fallout from reactor accidents or nuclear detonations. When ingested or inhaled, it can expose the entire body for an extended period of time, potentially contributing to serious health consequences ranging from acute radiation syndrome to increased cancer risks. In order to identify changes in gene expression that may be informative for detecting such exposure, and to begin examining the molecular responses involved, we have profiled global gene expression in mice injected with 137CsCl. more...

Organism:

Mus musculus

Type:

Expression profiling by array

Platform:

GPL10333

48 Samples

Download data: TXT

(Submitter supplied) Background: Ionizing Radiation (IR) is a known pro-inflammatory agent and in the process of development of biomarkers for radiation biodosimetry a chronic inflammatory disease condition could act as a confounding factor. Hence, it is important to develop radiation biodosimetry that can distinguish between IR-induced inflammatory responses and pre-existing disease. In this study, we compared the gene expression response of a genetically modified mouse model of inflammatory bowel disease (Il10-/-) with that of a normal wild-type mouse to potentially develop transcriptomic based biodosimetry markers for individual susceptibility to radiation. more...

Organism:

Mus musculus

Type:

Expression profiling by array

Platform:

GPL11202

20 Samples

Download data: TXT

(Submitter supplied) There is a perceived need to develop tools for high-throughput radiation biodosimetry. In the case of an improvised nuclear device or a dirty bomb in a major city, potentially hundreds of thousands of people would have to be screened to assign exposed individuals to appropriate treatment. Gene expression represents an emerging approach to biodosimetry and could potentially provide an estimate of both absorbed dose and individual radiation-induced injury. more...

Organism:

Mus musculus

Type:

Expression profiling by array

Platform:

GPL11202

32 Samples

Download data: TXT

(Submitter supplied) Background: The effects of dose-rate and its implications on radiation biodosimetry methods are not well studied in the context of large-scale radiological scenarios. There are significant health risks to individuals exposed to an acute dose in such an event, but the most realistic scenario would be a combination of exposure to both high and low dose-rates, from both external and internal radioactivity. more...

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL13497

35 Samples

Download data: TXT

(Submitter supplied) In the current study, we investigated the gene expression response of blood cells of mice that were injected with variable amounts of 137Cs We isolated total RNA from peripheral blood from mice with 0 (control), 158, 191, 215 and 259 uCi cesium source amount on days 2, 3, 5, 7 and 14 days after injection. Using Agilent Mouse Whole Genome microarrays, we identified x genes that were significantly differentially expressed across the 14-day time course of this study. more...

Organism:

Mus musculus

Type:

Expression profiling by array

Platform:

GPL11202

152 Samples

Download data: TXT

(Submitter supplied) Background: In the event of an improvised nuclear device or radiological dirty bomb detonation large numbers of people will be exposed to radiation that would require a timely and accurate biodosimetry to identify the highly exposed individuals who would require medical treatment from those who have received low or no radiation (the “worried well”). Gene expression signatures in response to radiation have been derived from mice and human peripheral blood and the impact of a number of variables, such as dose, time, genotype, and gender, on gene expression has been analyzed. more...

Organism:

Mus musculus

Type:

Expression profiling by array

Platform:

GPL11202

24 Samples

Download data: TXT

(Submitter supplied) Measuring global gene expression using cDNA or oligonucleotide microarrays is an effective approach to understanding the complex mechanisms of the effects of radiation. However, few studies have been carried out that investigate gene expression in vivo after prolonged exposure to low-dose-rate radiation. In this study, C57BL/6J mice were continuously irradiated with γ-rays for 485 days at dose-rates of 0.032 – 13 μGy/min. more...

Organism:

Mus musculus

Type:

Expression profiling by array

Platform:

GPL4234

24 Samples

Download data: CSV

(Submitter supplied) Time series for gene expression changes following 3 Gy and 10 Gy of ionizing radiation exposure. Keywords: time-course

Organism:

Homo sapiens

Type:

Expression profiling by array

Dataset:

GDS479

Platform:

GPL91

12 Samples

Download data

Temporal analysis of effect of 3 Gy and 10 Gy ionizing radiation (IR) exposure on lymphoblastoid cells. Various time points up to 24 hours examined.

Organism:

Homo sapiens

Type:

Expression profiling by array, count, 3 dose, 2 stress, 6 time sets

Platform:

GPL91

Series:

GSE701

12 Samples

Download data

(Submitter supplied) Analysis of human peripheral blood 48 hours after irradiation ex vivo with graded doses of gamma rays. Results have been used in building and testing classifiers to predict exposure dose for use in radiological triage, and also provide insight into immune cell responses. Results were compared with those from earlier times and from patients exposed in vivo.

Organism:

Homo sapiens

Type:

Expression profiling by array

Platforms:

GPL6480 GPL6848

75 Samples

Download data: TXT

(Submitter supplied) Circadian rhythm study on transcriptional responses to i.v. administered 90 kBq iodine-131 after 24h in mouse kidney cortex and medulla, liver, lungs, spleen, and thyroid. Female BALB/c nude mice (n=4/group) were i.v. injected with 90 kBq 131I at three different times of day, i.e. at 9.00 am, at 12.00 pm, or at 3.00 pm, and killed under anesthesia after 24h following treatment for excision of organs. more...

Organism:

Mus musculus

Type:

Expression profiling by array

Platform:

GPL6885

136 Samples

Download data: TXT

(Submitter supplied) To study the short term (48 h) hepatic transcriptional changes and identify potential modes of action, Atlantic salmon (Salmo salar) were exposed to 15 mGy, 70 mGy and 280 mGy external gamma radiation. A combination of high density (60 k) custom oligonucleotide salmonid microarray and quantitative real-time reverse transcription polymerase chain reaction (qPCR) was employed to perform gene expression analyses. more...

Organism:

Salmo salar; salmonid fish

Type:

Expression profiling by array

Platform:

GPL18864

16 Samples

Download data: TXT

(Submitter supplied) The objective of this investigation was to characterize, at individual level, the transcriptional response and the onset of regenerative processes in mouse skin irradiated with different doses of fast neutrons. We performed a high-throughput gene expression analysis, by DNA oligonucleotide microarray on 24 three months old C57Bl/6 mice irradiated with 0, 0,2 and 1 Gy of mono-energetic 14 MeV neutron. more...

Organism:

Mus musculus

Type:

Expression profiling by array

Platform:

GPL11211

8 Samples

Download data: GPR

(Submitter supplied) This study was designed to identify molecular changes induced by radiation in mouse lung.

Organism:

Mus musculus

Type:

Expression profiling by array

Platform:

GPL10333

9 Samples

Download data: TXT

(Submitter supplied) This SuperSeries is composed of the SubSeries listed below.

Organism:

Mus musculus

Type:

Expression profiling by array

Platform:

GPL6246

30 Samples

Download data: CEL