GEO DataSets

(Submitter supplied) In this study, the A-NO2- sensitivity of mucA22 mutant bacteria vs. a genome sequence-confirmed mucA deletion (∆mucA) mutant was determined. The ∆mucA is surprisingly more resistant to A-NO2- than mucA22 bacteria. The genes responsible for the disposal of nitric oxide (NO), a by-product of A-NO2-, were found to be near wild-type levels in the ∆mucA mutant yet were dramatically reduced in the mucA22 mutant.

Organism:

Pseudomonas aeruginosa; Pseudomonas aeruginosa PAO1

Type:

Expression profiling by array

Platform:

GPL84

9 Samples

Download data: CEL, CHP

(Submitter supplied) Pseudomonas aeruginosa (P. aeruginosa) lung infection is a significant cause of mortality in patients with cystic fibrosis (CF). Most CF patients acquire unique P. aeruginosa strains from the environment; however clonal strains have been identified in CF communities in several countries. Two clonal strains infect 10% to 40% of patients in three CF clinics in mainland eastern Australia. The expression profiles of four planktonically-grown isolates of one Australian clonal strain (AES-2), and four non–clonal CF P. more...

Organism:

Pseudomonas aeruginosa

Type:

Expression profiling by array

Platform:

GPL84

8 Samples

Download data: CEL, CHP

(Submitter supplied) Pseudomonas aeruginosa strains PAHM4 and PAO1 were grown at 37C on LB and RNA was hybridized on the Affymetrix P. aeruginosa chip to compare transcript differences from a BQ isolate to a well characterized wound isolate.

Organism:

Pseudomonas aeruginosa PAO1; Pseudomonas aeruginosa PAHM4; Pseudomonas aeruginosa

Type:

Expression profiling by array

Platform:

GPL84

6 Samples

Download data: CEL

(Submitter supplied) Purpose : The goal of this study was to use RNA Seq to define the regulon of the transciption factor Anr by comparing global transcriptional profiles of Pseudomonas aeruginosa strain PAO1 and a clinical isolate with their isogenic ∆anr mutants, grown in colony biofilms at 1% oxygen. Methods : mRNA profiles were generated for laboratory strain PAO1 and for a clinical isolate J215, as well as for ∆anr derivatives of each strain, in duplicate, by deep sequencing. more...

Organism:

Pseudomonas aeruginosa PAO1; Pseudomonas aeruginosa

Type:

Expression profiling by high throughput sequencing

Platforms:

GPL18782 GPL18287

8 Samples

Download data: XLSX

(Submitter supplied) In order to understand how Pseudomonas aeruginosa responds to low oxygen we grew strain PAO1 with 3 different oxygen concentrations: 2%, 0.4% and 0% supplemented with nitrate as an electron acceptor. Gene expression under these conditions was compared to that of cells grown with 20% oxygen. Keywords: Comparison of transcriptome profiles

Organism:

Pseudomonas aeruginosa

Type:

Expression profiling by array

Dataset:

GDS2893

Platform:

GPL84

8 Samples

Download data: CEL

Analysis of Pseudomonas aeruginosa strain PAO1 grown with 2% or 0.4% oxygen (O2), or without O2 but with nitrate instead. In cystic fibrosis (CF) lungs, PAO1 grows to high densities in mucopurulent material depleted in O2. Results provide insight into mode of growth in CF lungs.

Organism:

Pseudomonas aeruginosa

Type:

Expression profiling by array, count, 4 dose, 3 stress sets

Platform:

GPL84

Series:

GSE6741

8 Samples

Download data: CEL

(Submitter supplied) A hallmark of the biofilm architecture is the presence of microcolonies. However, little is known about the underlying mechanisms governing microcolony formation. In the human pathogen Pseudomonas aeruginosa, microcolony formation is dependent on the two-component regulator MifR, with mifR mutant biofilms exhibiting an overall thin structure lacking microcolonies, and overexpression of mifR resulting in hyper-microcolony formation. more...

Organism:

Pseudomonas aeruginosa

Type:

Expression profiling by array

Platform:

GPL84

6 Samples

Download data: CEL, CHP

(Submitter supplied) Antibiotic-resistant “super-bug” bacteria represent a global health problem with no imminent solutions. Here, we demonstrate that AB569 (acidified nitrite (A-NO2-) and Na2-EDTA) killed all Gram-negative and Gram-positive bacteria tested. AB569 was also efficacious at killing the model organism P. aeruginosa in biofilms and in a murine chronic lung infection model. AB569 was not toxic to human cell lines at bactericidal concentrations. more...

Organism:

Pseudomonas aeruginosa PAO1

Type:

Expression profiling by high throughput sequencing

Platform:

GPL18782

11 Samples

Download data: TXT

(Submitter supplied) The response of bacteria to the conditions at the site of infection is a key part of the transcriptional program that will determine the sucess of the infectious agent. To model the environment of the distal airway, we used bovine pulmonary surfactant (Survanta). P. aeruginosa transcript levels were measured in the presence or absence of Survanta in MOPS minimal medium to identify transcripts altered in response to surfactant. more...

Organism:

Pseudomonas aeruginosa

Type:

Expression profiling by array

Datasets:

GDS4958 GDS4959

Platform:

GPL84

8 Samples

Download data: CEL

Analysis of P. aeruginosa sphR null mutants grown in media containing lung surfactant. P. aeruginosa is an opportunistic pathogen of the human lung. SphR is an AraC-family transcription factor. Results provide insight into the role of sphR in P. aeruginosa infections in the lung.

Organism:

Pseudomonas aeruginosa

Type:

Expression profiling by array, transformed count, 2 agent, 2 genotype/variation sets

Platform:

GPL84

Series:

GSE48982

8 Samples

Download data: CEL

Analysis of P. aeruginosa grown in media containing lung surfactant. P. aeruginosa is a common environmental bacterium that is also an opportunistic pathogen, particularly of the human lung. Results provide insight into the mechanisms used by P. aeruginosa to establish and maintain lung infections.

Organism:

Pseudomonas aeruginosa

Type:

Expression profiling by array, transformed count, 2 agent sets

Platform:

GPL84

Series:

GSE48982

4 Samples

Download data: CEL

(Submitter supplied) Pseudomonas aeruginosa (PA) is an opportunistic pathogen frequently isolated from cutaneous chronic wounds. How PA, in the presence of oxidative stress (OS), colonizes chronic wounds and forms a biofilm is still unknown. The purpose of this study is to investigate the changes in gene expression seen when PA is challenged with the high levels of OS present in chronic wounds. We used a biofilm-forming PA strain isolated from the chronic wounds of our murine model (RPA) and performed a qPCR to obtain gene expression patterns as RPA developed a biofilm in vitro in the presence of high levels of OS, and then compared the findings in vivo, in our mouse model of chronic wounds. more...

Organism:

Pseudomonas aeruginosa

Type:

Expression profiling by high throughput sequencing

Platform:

GPL33271

18 Samples

Download data: TXT