GEO DataSets

(Submitter supplied) There is a growing interest for the use of post-fermentation mycelial waste to obtain cell wall chitin as an added-value product. In the pursuit to identify suitable production strains that can be used for post-fermentation cell wall harvesting, we turned to an Aspergillus niger strain in which the kexB (also named pclA in literature) gene was deleted. Previous work has shown that deletion of kexB causes hyper-branching and thicker cell walls, which is beneficial as these properties reduce fermentation viscosity and lysis. more...

Organism:

Aspergillus niger

Type:

Expression profiling by high throughput sequencing

Platform:

GPL25519

4 Samples

Download data: TSV

(Submitter supplied) In this study, we screened morphological mutants in the filamentous fungus N. crassa. Of the 90 morphological mutants screened, 14 mutants exhibited considerably higher viscosity compared with that of the wild type strain, and only 2 mutants showed low-viscosity morphologies in submerged culture. We observed that disruption of gul-1 (NCU01197), which encodes an mRNA binding protein involved in cell wall remodeling, caused pellet formation as the fermentation progressed, and resulted in the most significant decrease in viscosity of culture broth. more...

Organism:

Neurospora crassa

Type:

Expression profiling by high throughput sequencing

Platform:

GPL16164

2 Samples

Download data: XLSX

(Submitter supplied) Wild type (CAI10) and phr1 null mutant (CAS10) were analyzed at 0, 1, 3 and 5 hours after the shift of blastospores to Hyphae inducing conditions

Organism:

Candida albicans

Type:

Expression profiling by array

Platform:

GPL9545

24 Samples

Download data: TXT

(Submitter supplied) In this study, we compared the gene expression pattern of A. niger grown in liquid sugar beet pulp (SBP) at different time points, a by-product of the sugar industry that consists mainly of cellulose, xyloglucan, and pectin. Finally, we compared A. niger genetic response to liquid SBP to that of the same fungus when grown on solid SBP plates and polygalacturonic acid (PGA).

Organism:

Aspergillus niger; Aspergillus niger CBS 513.88

Type:

Expression profiling by array; Third-party reanalysis

Platform:

GPL6758

8 Samples

Download data: CEL, TXT

(Submitter supplied) We did transcription profiling on the effect of glucosamine in Saccharomyces cerevisiae using Research Genetics strains BY4741 (wild type) and 5251 (fks1). Yeast cells exposed to glucosamine in YPD growth medium show a significant increase in chitin content in the lateral cell wall. Likewise, cell wall stress caused by a gene deletion e.g., fks1 also results in elevated chitin. By comparing our data for fks1 with those from the literature we confirmed a strong induction of genes responsive to cell wall integrity, environmental stress as well as genes involved in cell signaling. more...

Organism:

Saccharomyces cerevisiae

Type:

Expression profiling by array

Dataset:

GDS344

Platform:

GPL90

11 Samples

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Investigation into chitin synthesis in wildtype BY4741 and mutant fks1 (elevated chitin). Cells exposed to chitin-inducing glucosamine either for 2 hours, or continuous steady state.

Organism:

Saccharomyces cerevisiae

Type:

Expression profiling by array, count, 3 protocol, 2 strain sets

Platform:

GPL90

Series:

GSE441

11 Samples

Download data

(Submitter supplied) Chitin is an important fungal cell wall component that is cross-linked to β-glucan for structural integrity. Acquisition of chitin to glucan cross-links has previously been shown to be performed by transglycosylation enzymes in S. cerevisiae, called Congo Red hypersensitive (Crh) enzymes. Here, we characterized the impact of deleting all seven gene members of the crh gene family (crhA-G) in Aspergillus niger on cell wall integrity, cell wall composition and genome-wide gene expression. more...

Organism:

Aspergillus niger

Type:

Expression profiling by high throughput sequencing

Platform:

GPL21090

8 Samples

Download data: TXT