GEO DataSets

(Submitter supplied) We report the results of gene expression profiling via RNA-sequencing of primary human choroid plexus epithelial cells in vitro that were infected with Borrelia burgdorferi, the causative agent of Lyme disease. Epithelial cells were cultured with B. burgdorferi for 48 hours prior to RNA isolation. Following high-throughput sequencing via Illumina HiSeq 4000, a total of 258 differentially expressed genes were identified. more...

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL20301

6 Samples

Download data: TXT

(Submitter supplied) Purpose: Studies on gene expression changes in joint and heart tissues of laboratory mice have provided insights into the localized host responses to Borrelia burgdorferi colonization; however, gene expression changes in the CNS of mice during B. burgdorferi infection remain unclear. Therefore, we took an unbiased approach to examine potential changes in the dura mater as well as the brain cortex and hippocampus at day 7 post-infection using RNA sequencing (RNA-seq). more...

Organism:

Mus musculus

Type:

Expression profiling by high throughput sequencing

Platform:

GPL21103

24 Samples

Download data: TXT

(Submitter supplied) This SuperSeries is composed of the SubSeries listed below.

Organism:

Homo sapiens

Type:

Expression profiling by high throughput sequencing; Non-coding RNA profiling by high throughput sequencing

Platforms:

GPL11154 GPL15520

15 Samples

Download data: TXT

(Submitter supplied) mRNA profiles of astrocytes infected with Borrelia burdorferi for 24 hours, 48 hours, and 24 hour uninfected controls were generated by deep sequencing, in triplicate, using Illumina HiSeq.

Organism:

Homo sapiens

Type:

Expression profiling by high throughput sequencing

Platform:

GPL11154

9 Samples

Download data: TXT

(Submitter supplied) miRNA profiles of astrocytes infected with Borrelia burgdorferi for 24 hours, 48 hours, and 24 hour uninfected controls were generated by deep sequencing, in duplicate, using Illumina MiSeq.

Organism:

Homo sapiens

Type:

Non-coding RNA profiling by high throughput sequencing

Platform:

GPL15520

6 Samples

Download data: CSV

(Submitter supplied) We used microarrays to detail the global gene expression changes following apical infection of porcine choroid plexus epithelial cells (PCPEC) with Streptococcus suis (S. suis)

Organism:

Sus scrofa

Type:

Expression profiling by array

Dataset:

GDS4245

Platform:

GPL3533

6 Samples

Download data: CEL, CHP

Analysis of porcine choroid plexus epithelial cells (PCPEC) infected with virulent Streptococcus suis serotype 2 and isogenic capsular mutant 10cps{delta}EF. The S. suis capsule is critical to its pathogenicity. Results provide insight into choroid plexus cellular response to S. suis meningitis infe

Organism:

Sus scrofa

Type:

Expression profiling by array, count, 3 infection sets

Platform:

GPL3533

Series:

GSE23751

6 Samples

Download data: CEL, CHP

(Submitter supplied) Background: In aging and Alzheimer’s disease there are striking changes in CSF composition that may relate to altered choroid plexus function. Studying CP tissue gene expression at the BCSFB provides further insight into epithelial and stromal responses to aging and diseased states. Results: Highly significant differences in gene expression occurred in CP of advanced AD patients vs. normal controls. more...

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL10379

20 Samples

Download data: CEL

(Submitter supplied) Transcriptional profiling of NHDF Cells comparing control untreated fibroblasts with fibroblasts coincubated with three different species of the Borrelia burgdorferi sensu lato group.

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL10558

24 Samples

Download data: IDAT, TXT

(Submitter supplied) Adiponectin-mediated pathways contribute to mammalian homeostasis; however, little is known about adiponectin and adiponectin receptor signaling in arthropods. In this study, we demonstrate that Ixodes scapularis ticks have an adiponectin receptor-like protein (ISARL) but lack adiponectin, suggesting activation by alternative pathways. ISARL expression is significantly upregulated in the tick gut after Borrelia burgdorferi infection, suggesting that ISARL signaling may be co-opted by the Lyme disease agent. more...

Organism:

Ixodes scapularis

Type:

Expression profiling by high throughput sequencing

Platform:

GPL29893

18 Samples

Download data: TXT

(Submitter supplied) Splenocytes comprised a wide-range of immune cells responsible for the protection against infectious diseases. We exposed splenocytes to GFP+ Borrelia, sorted GFP+ and GFP- splenocytes and performed single-cell RNA seq to analyze the DEGs between infected and bystander population in different immune cell populations

Organism:

Mus musculus

Type:

Expression profiling by high throughput sequencing

Platform:

GPL24247

4 Samples

Download data: MTX, TSV

(Submitter supplied) Dendritic cells bridge the innate and adaptive immune response by serving as sensors of infection and as the primary antigen presenting cells responsible for the initiation of the T cell response against invading pathogens. Initial interactions between B. burgdorferi, the causative agent of Human Lyme disease, and dendritic cells remain largely unexplored. To address this, we cultured monocyte-derived dendritic cells (moDCs) with and without the Toll-Like Receptor 2 agonist LTA or live B. more...

Organism:

Homo sapiens

Type:

Expression profiling by high throughput sequencing

Platform:

GPL24676

91 Samples

Download data: TXT

(Submitter supplied) Borreliella burgdorferi is the causative agent of Lyme disease, which is the most common vector-borne disease in the United States. While cases of Lyme disease are geographically limited to the Northeast, mid-Atlantic, and Midwest states, there are still estimates of almost 500,000 cases annually in the United States alone. In this study, we sought to assess transcriptional changes that occur in response to B. more...

Organism:

Mus musculus

Type:

Expression profiling by high throughput sequencing

Platform:

GPL19057

10 Samples

Download data: H5