GEO DataSets

(Submitter supplied) In bacteria, phenotypic heterogeneity rescues the need for diversity in isogenic populations and allow concomitant multiple survival strategies when choosing only one is too risky. This powerful tactic is exploited for competence in streptococci and results in a bimodal activation, where only a subset of the community triggers the system. Deciphering the mechanisms underlying this puzzling behavior has remained challenging, especially since its study has been mainly achieved in S. more...

Organism:

Streptococcus salivarius

Type:

Expression profiling by high throughput sequencing

Platform:

GPL23620

3 Samples

Download data: XLSX

(Submitter supplied) Synchronizing production of antibacterial compounds and integration of DNA released by dead cells, competence is one of the most efficient bacterial evolutionary and adaptative strategies. In most streptococci, this tactic is orchestrated by the ComRS system, a pheromone communication device providing competence bimodal initiation and sharp time window of activation. Understanding how this developmental process integrates multiple inputs to fine-tune the adequate response is a long-standing question. more...

Organism:

Streptococcus salivarius

Type:

Expression profiling by high throughput sequencing

Platform:

GPL32298

3 Samples

Download data: XLSX

(Submitter supplied) S. mutans UA159 was cultivated in chemically defined medium. Changes in the transcriptome in response to the autoinducing peptides CSP and XIP were monitored for 30 minutes.

Organism:

Streptococcus mutans UA159

Type:

Expression profiling by high throughput sequencing

Platform:

GPL19789

20 Samples

Download data: TXT

(Submitter supplied) Small distortions in transcriptional networks might lead to drastic phenotypical changes, especially in cellular developmental programs such as competence for natural transformation. Here, we report a pervasive circuitry rewiring for competence and predation interplay in commensal streptococci. Canonically, in model species of streptococci such as Streptococcus pneumoniae and Streptococcus mutans, the pheromone-based two-component system BlpRH is a central node that orchestrates the production of antimicrobial compounds (bacteriocins) and incorporates signal from the competence activation cascade. more...

Organism:

Streptococcus salivarius

Type:

Expression profiling by high throughput sequencing; Non-coding RNA profiling by high throughput sequencing

Platform:

GPL23620

5 Samples

Download data: XLS

(Submitter supplied) Whole genome expression profile comparing MGAS315 treated with XIP pheromone versus vehicle-treated cells Interpretations are described further in the manuscript to be submitted: authors Mashburn-Warren, Morrison, and Federle. Title: The Cryptic Competence Pathway in Streptococcus pyogenes is Controlled by a Peptide Pheromone.

Organism:

Streptococcus pyogenes MGAS315; Streptococcus pyogenes NZ131

Type:

Expression profiling by genome tiling array

Platform:

GPL15576

2 Samples

Download data: CALLS, PAIR, TXT

(Submitter supplied) We investigated the covS-regulation of S. pyogenes in a hypervirulent M23 strain using RNA-sequencing. The differential gene expression comparison between the covS- mutant and isogenic wild type covS+ identified altered expression of 349 (18%) genes, including a broad spectrum of virulence genes and diverse metabolic genes. The data showed that the strain achieved hypervirulence by enhancing the expression of genes responsible for invasiveness and antiphagocytosis (i.e., hasABC), by abrogating the expression of toxic genes (i.e., speB), and by compromising gene products with dispensable functions (i.e., sfb1). more...

Organism:

Streptococcus pyogenes

Type:

Expression profiling by high throughput sequencing

Platform:

GPL19982

8 Samples

Download data: XLS

(Submitter supplied) The human oral cavity is one of the most competing environments, considering the extent and diversity of the bacterial community that colonizes it, as well as the multiple stresses to which it is subjected. The commensal species Streptococcus salivarius is one of the first colonizers of the oral mucosa (tongue, gums, inner cheeks and tooth enamel) in infants and remains predominant throughout the life of an adult. more...

Organism:

Streptococcus salivarius

Type:

Expression profiling by high throughput sequencing

Platform:

GPL23620

6 Samples

Download data: XLSX

(Submitter supplied) Transcriptional profiling of S. thermophilus LMD-9 wild-type compared to the isogenic mutant strain CB003 (deletion of comX, STER_0189) for the identification of the ComX regulon. Cells were grown in CDM medium supplemented with lactose 1% (CDML) and sampled at OD600 = 0.4 for mRNA extraction

Organism:

Streptococcus thermophilus LMD-9; Streptococcus thermophilus

Type:

Expression profiling by array

Platform:

GPL13365

3 Samples

Download data: TXT

(Submitter supplied) Transcriptional profiling of S. thermophilus LMD-9 wild-type compared to the isogenic mutant strain CB052 (deletion of mecA, STER_0216) for the identification of the MecA regulon. Cells were grown in THB medium supplemented with glucose 1% (THBG) and sampled at OD600 = 0.4 for mRNA extraction

Organism:

Streptococcus thermophilus; Streptococcus thermophilus LMD-9

Type:

Expression profiling by array

Platform:

GPL13365

3 Samples

Download data: TXT

(Submitter supplied) Transcriptional profiling of S. thermophilus LMD-9 wild-type in the presence of mitomycin C. Cells were grown in CDM medium supplemented with lactose 1% (CDML), without or with mitomycin C 0.08 microg/ml, and sampled at OD600 = 0.4 for mRNA extraction.

Organism:

Streptococcus thermophilus; Streptococcus thermophilus LMD-9

Type:

Expression profiling by array

Platform:

GPL13365

3 Samples

Download data: TXT

(Submitter supplied) Transcriptional profiling of S. thermophilus LMD-9 wild-type compared to the isogenic mutant strain CB0082 (deletion of hdiR, STER_0918) for the identification of the HdiR regulon. Cells were grown in CDM medium supplemented with lactose 1% (CDML) and sampled at OD600 = 0.4 for mRNA extraction

Organism:

Streptococcus thermophilus LMD-9; Streptococcus thermophilus

Type:

Expression profiling by array

Platform:

GPL13365

3 Samples

Download data: TXT

(Submitter supplied) We sought to determine how CovRS mutations varying CovR phosphorylation levels affect the gene expression profile of group A streptococcus

Organism:

Streptococcus sp. 'group A'

Type:

Expression profiling by high throughput sequencing

Platform:

GPL19691

32 Samples

Download data: TXT

(Submitter supplied) In Streptococcus mutans, an oral colonizer associated with dental caries, competence for natural transformation can be triggered by both CSP and XIP pheromones. Competence induced by CSP is a late response that requires induction of the XIP encoding gene comS, but the mechanism(s) linking the two systems remains unknown. To learn how the CSP and XIP pheromone regulatory pathways are temporally linked, we mapped the global changes in gene expression at early and late phases of the CSP response and investigated the effect of deletion of comS on the S. more...

Organism:

Streptococcus mutans UA159

Type:

Expression profiling by genome tiling array

Platform:

GPL20590

6 Samples

Download data: PAIR, TXT

(Submitter supplied) Two covR mutant derivatives of parental strain MGAS2221 were recovered from mice experimentally infected with MGAS2221 and shown to differ in terms of the number and concentration of secreted proteins. One of the covR mutant strains had a secretion phenotype identical to a covS mutant strain, while the other had a secretion phenotype identical to a constructed covR mutant strain. To further investigate the potential differences between the two covR mutant strains we performed expression microarray analysis.

Organism:

Coxiella burnetii; Chlamydia trachomatis; Streptococcus pyogenes; Borreliella burgdorferi; Yersinia pestis; Staphylococcus aureus

Type:

Expression profiling by array

Platform:

GPL2129

4 Samples

Download data: CEL

(Submitter supplied) Bacteriocins are natural antimicrobial peptides produced by a bacterium to kill closely related competitors. Streptococcus gallolyticus subsp. gallolyticus (Sgg) UCN34 produces a two-component bacteriocin named gallocin to colonize the gut by killing resident enterococci. In the present work, we investigated how gallocin was regulated by deleting individually three genes present in the locus and encoding potentially an inducing peptide (gsp), a histidine kinase (ghk) and a LytTR-containing response regulator (grr). more...

Organism:

Streptococcus gallolyticus UCN34

Type:

Expression profiling by high throughput sequencing

Platform:

GPL28370

12 Samples

Download data: TXT

(Submitter supplied) The mechanisms by which bacteria sense the host environment and alter gene expression are poorly understood. LiaFSR is a gene regulatory system unique to Gram-positive bacteria including group A Streptococcus (GAS) and responds to cell envelope stress. We previously showed that LiaF acts as an inhibitor to LiaFSR activation in GAS. To better understand gene regulation associated with LiaFSR activation, we performed RNA-sequencing on isogenic deletion mutants fixed in a LiaFSR “always on” (DliaF) or “always off” (DliaR) state. more...

Organism:

Streptococcus pyogenes

Type:

Expression profiling by high throughput sequencing

Platform:

GPL24912

9 Samples

Download data: XLSX

(Submitter supplied) This SuperSeries is composed of the SubSeries listed below.

Organism:

Pseudomonas aeruginosa

Type:

Expression profiling by array

Platform:

GPL84

24 Samples

Download data: CEL, CHP

(Submitter supplied) The Pseudomonas aeruginosa MvfR-dependent QS regulatory pathway controls the expression of key virulence genes; and is activated via the extracellular signals 4-hydroxy-2-heptylquinoline (HHQ) and 3,4-dihydroxy-2-heptylquinoline (PQS). Our findings reveal a multi-layered homeostatic regulation of PQS and HHQ and the MvfR regulon component PqsE which is a key mediator in orchestrating this homeostatic regulation.

Organism:

Pseudomonas aeruginosa

Type:

Expression profiling by array

Platform:

GPL84

15 Samples

Download data: CEL, CHP

(Submitter supplied) The Pseudomonas aeruginosa MvfR-dependent QS regulatory pathway controls the expression of key virulence genes; and is activated via the extracellular signals 4-hydroxy-2-heptylquinoline (HHQ) and 3,4-dihydroxy-2-heptylquinoline (PQS), whose syntheses depend on anthranilic acid (AA), the primary precursor of 4-hydroxy-2-alkylquinolines (HAQs). We identified halogenated AA analogs that specifically inhibited HAQ biosynthesis and disrupted MvfR-dependent gene expression. more...

Organism:

Pseudomonas aeruginosa

Type:

Expression profiling by array

Platform:

GPL84

10 Samples

Download data: CEL, CHP

(Submitter supplied) The goal of this study were to compare the transcriptoms of CcpA and CovR in group A Streptococcus. Isogenic mutant strains were created in which CcpA and CovR were inactivated alone and in combination. The transcriptomes of the four strains (wild-type, CcpA mutant, CovR mutant, and ccpA-covR double mutant) were then compared.

Organism:

Streptococcus pyogenes; Streptococcus sp. 'group A'

Type:

Expression profiling by array

Platform:

GPL9080

32 Samples

Download data: CEL