GEO DataSets

(Submitter supplied) We studied cell lines derived from two transgenic mammary tumors driven by human HER2 that showed different dynamics of HER2 status. MamBo89 (HER2 stable) cell line displayed high and stable HER2 expression, which was maintained upon in vivo passages, whereas MamBo43 (HER2 labile) cell line gave rise to HER2-negative tumors, from which MamBo38 (HER2 loss) cell line was derived. MamBo cell lines were established from mammary tumors of FVBhuHER2 virgin female mice.

Organism:

Mus musculus

Type:

Expression profiling by high throughput sequencing

Platform:

GPL19057

11 Samples

Download data: TXT

(Submitter supplied) HER2 (ERBB2) gene amplification and PIK3CA mutations often co-occur in breast cancer, and aberrant activation of the PI3K pathway has been implicated in resistance to HER2-directed therapies. We have created a mouse model of HER2-overexpressing (HER2+), PIK3CAH1047R-mutant breast cancer. Mice expressing both human HER2 and mutant PIK3CA in their mammary glands developed tumors with a significantly shorter latency compared to mice expressing either oncogene alone. more...

Organism:

Mus musculus

Type:

Expression profiling by array

Platform:

GPL11383

19 Samples

Download data

(Submitter supplied) This SuperSeries is composed of the SubSeries listed below.

Organism:

Mus musculus

Type:

Non-coding RNA profiling by array; Expression profiling by array; Genome variation profiling by genome tiling array

Platforms:

GPL2884 GPL4134 GPL16199

96 Samples

Download data: TXT

(Submitter supplied) miRNA profiling of normal and tumorigenic mouse mammary tissue. Mouse genotypes consist of wildtype, MMTV-Met, MMTV-Met;Trp53fl/+;Cre, and Trp53fl/+;Cre.

Organism:

Mus musculus

Type:

Non-coding RNA profiling by array

Platform:

GPL16199

25 Samples

Download data: TXT

(Submitter supplied) Transcriptional profiling of normal and tumorigenic mouse mammary tissue. Mouse genotypes consist of wildtype, MMTV-Met, MMTV-Met;Trp53fl/+;Cre, and Trp53fl/+;Cre.

Organism:

Mus musculus

Type:

Genome variation profiling by genome tiling array

Platform:

GPL2884

30 Samples

Download data: TXT

(Submitter supplied) Transcriptional profiling of normal and tumorigenic mouse mammary tissue. Mouse genotypes consist of wildtype, MMTV-Met, MMTV-Met;Trp53fl/+;Cre, and Trp53fl/+;Cre.

Organism:

Mus musculus

Type:

Expression profiling by array

Platform:

GPL4134

41 Samples

Download data: TXT

(Submitter supplied) Molecular profiling was used to classify mammary tumors that develop in MTB-IGFIR transgenic mice. It was determined that the primary mammary tumors (PMT), which develop due to elevated expression of the type I insulin-like growth factor receptor (IGF-IR) in mammary epithelial cells, most closely resemble murine tumors with basal-like or mixed gene expression profiles and with human basal-like breast cancers. more...

Organism:

Mus musculus

Type:

Expression profiling by array

Platform:

GPL7202

28 Samples

Download data: TXT

(Submitter supplied) WAP-Cre:Ptenf/f:p53lox.stop.lox_R270H composite mice were generated by genetic crossing. In these mice, Pten is deleted and a R270H p53 mutation in the DNA binding domain is induced upon expression of Cre recombinase in pregnancy-identified alveolar progenitors. Tumors were characterized by histology, marker analysis, various bioinformatics methods, high-throughput (HTP) FDA-drug screen as well as orthotopic injection to quantify tumor initiating cells (TICs) and tail-vein injection to identify lung-metastasis. more...

Organism:

Mus musculus

Type:

Expression profiling by array

Platform:

GPL6246

31 Samples

Download data: CEL

(Submitter supplied) d16HER2 is a splice variant of HER2 receptor characterized by a significant tumor aggressiveness. We derived primary tumor cell lines from spontaneous tumors grown in mice transgenic respectively for human d16 (MI6 and MI7 cell lines) and WT HER2 isoforms (WTHER2_1 and WTHER2_2). To analyze the molecular mechanisms underlying the higher tumorigenicity of d16HER2 than that of WTHER2, we conducted gene expression profiling analysis of these cell lines.

Organism:

Mus musculus

Type:

Expression profiling by array

Platform:

GPL6887

16 Samples

Download data: TXT

(Submitter supplied) We used microarrays to investigate the transcription profile of FOXC2 expression in a human mammary epithelial cell line.

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL570

6 Samples

Download data: CEL

(Submitter supplied) HER2 targeting with trastuzumab has changed the prognosis of breast cancer patients carrying amplification and/or overexpression of this oncogene. Despite this progress, however, resistance to trastuzumab occurs in the vast majority of patients. Newer anti-HER2 therapies, like the dual tyrosine-kinase inhibitor (TKI) lapatinib, show antitumor activity in a limited proportion of patients, indicating that HER2 can be still exploited as a target after trastuzumab failure. more...

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL4133

2 Samples

Download data: TXT

(Submitter supplied) Most solid tumors seem to be organized in a hierarchy in which only a fraction of cells, termed tumor-initiating cells (TICs), is capable of disseminating new tumors after transplantation into recipient mice. However, whether a single TIC can induce a tumor or whether it requires additional TICs or non-TICs for tumor initiation is not known. Here we show that injections of single CD24+:Jag1- cells from Her2/Neu+ mammary tumors into recipient mammary glands induced tumors at a frequency of 1/22. more...

Organism:

Mus musculus

Type:

Expression profiling by array

Dataset:

GDS4099

Platform:

GPL6885

16 Samples

Download data: TXT

(Submitter supplied) The cancer stem cell model maintains that tumors are organized in a hierarchy driven by tumor initiating cells (TICs), and that patient survival inversely correlates with TIC gene expression. Here we generated a prognostic signature for HER2+ breast cancer from TICs purified from MMTV-Her2/Neu mammary tumors. TICs from this model, identified as Lin-:CD24+:JAG1- at a frequency of 2-5% by serial and single cell transplantation assays, showed elevated expression of proliferation genes and low expression of differentiation genes (compared to non-TIC fraction CD24- of the same tumor). more...

Organism:

Mus musculus

Type:

Expression profiling by array

Platform:

GPL6246

8 Samples

Download data: CEL

Analysis of primary MMTV-Neu mammary tumors and secondary tumors generated by lin- cell transplantation or by single tumor-initiating cell (TIC) transplantation. Results enable molecular comparisons between the primary and secondary tumors.

Organism:

Mus musculus

Type:

Expression profiling by array, count, 16 specimen, 3 tissue sets

Platform:

GPL6885

Series:

GSE29616

16 Samples

Download data

(Submitter supplied) In order to better understand signaling events following receptor dimerization involving HER2, we have generated an experimental system in which ErbB dimerization can be controlled. We used gene expression microarrays to identify genes and pathways that are differentially activated by HER2 homodimers and HER2 containing heterodimers.

Organism:

Homo sapiens

Type:

Expression profiling by array

Dataset:

GDS4361

Platform:

GPL6244

12 Samples

Download data: CEL, CHP

Analysis of mammary epithelial MCF10A/HER2/FKBP/HA cells treated with AP1510 to induce the chimeric HER2-FKBP molecule to homodimerize, or with TGFα or heregulin to induce heterodimerization with EGFR or HER3. Results provide insight into signaling events following HER2 receptor dimerization.

Organism:

Homo sapiens

Type:

Expression profiling by array, transformed count, 4 agent sets

Platform:

GPL6244

Series:

GSE22288

12 Samples

Download data: CEL, CHP

(Submitter supplied) The diversity of human breast cancer subtypes has led to the hypothesis that breast cancer is a number of different diseases arising from cells at various stages of differentiation. We have derived clonal multipotent metastatic mammary cancer stem cells from the polyomavirus middle T mouse model of breast cancer, that can differentiate into luminal, myoepithelial and alveolar cells. When injected orthotopically at low-density, the resulting tumors express estrogen and progesterone receptors. more...

Organism:

Mus musculus

Type:

Expression profiling by array

Platform:

GPL15887

43 Samples

Download data: PAIR

(Submitter supplied) A survey of the somatic allelic imbalances and copy number alterations in HER2-amplified breast cancer.

Organism:

Homo sapiens

Type:

Genome variation profiling by SNP array; SNP genotyping by SNP array

Platforms:

GPL13314 GPL8882

26 Samples

Download data: TXT

(Submitter supplied) The tyrosine kinase receptors HER2 and HER3 play an important role in breast cancer. The HER2/HER3 heterodimer is a critical oncogenic unit associated with reduced relapse-free and decreased overall survival. We provide gene expression profile of the mammary epithelial cells MCF10A expressing HER2, HER3 or HER2/HER3 and grown in three-dimensional cultures for 15 days in the presence of heregulin, a known HER3-ligand that stabilizes and activates the HER2/HER3 heterodimer.

Organism:

Homo sapiens

Type:

Expression profiling by array

Dataset:

GDS4359

Platform:

GPL6244

8 Samples

Download data: CEL

Analysis of MCF10A mammary epithelial cells expressing HER2, HER3, or HER2/HER3 heterodimer. Co-expression of HER2 and HER3 induced migration and invasion of MCF10A cells. Results provide insight into the role of HER2 and HER3 in breast cancer.

Organism:

Homo sapiens

Type:

Expression profiling by array, transformed count, 4 genotype/variation sets

Platform:

GPL6244

Series:

GSE37009

8 Samples

Download data: CEL